Transgenic Core

Equipment/facility: Facility

    Description

    The mission of the core is to create and preserve lines of genetically engineered rodents. The core can generate conventional transgenic mice and rats and can create chimeric mice harboring select genetic mutations. The core performs gene targeting in mouse embryonic stem cells, expansions of in-house targeted ES cell clones and KOMP derived clones, and preparation of targeted ES cell clones for microinjection.

    The core can rederive lines of mice from conventional housing to an SPF barrier status and can cryopreserve sperm, embryos, and ovaries from select mouse lines. Also, the core can reconstitute lines of mice from cryopreserved gametes by either simple embryo transfers or by performing in vitro fertilization and embryo transfer

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    Clone Cells
    Embryo Transfer
    Transgenic Rats
    Gene Targeting
    Microinjections
    Fertilization in Vitro
    Germ Cells
    Transgenic Mice
    Spermatozoa
    Ovary
    Rodentia
    Embryonic Structures
    Mutation
    Mouse Embryonic Stem Cells