Research Output 1979 2019

2019

A Bacterial Effector Mimics a Host HSP90 Client to Undermine Immunity

Lopez, V. A., Park, B. C., Nowak, D., Sreelatha, A., Zembek, P., Fernandez, J., Servage, K. A., Gradowski, M., Hennig, J., Tomchick, D. R., Pawłowski, K., Krzymowska, M. & Tagliabracci, V. S., Sep 19 2019, In : Cell. 179, 1, p. 205-218.e21

Research output: Contribution to journalArticle

Protein Kinases
Immunity
Phosphorylation
Pathogens
Plant Immunity
1 Citation (Scopus)

A composition-dependent molecular clutch between T cell signaling condensates and actin

Ditlev, J. A., Vega, A. R., Köster, D. V., Su, X., Tani, T., Lakoduk, A. M., Vale, R. D., Mayor, S., Jaqaman, K. & Rosen, M. K., Jul 3 2019, In : eLife. 8

Research output: Contribution to journalArticle

Open Access
Cell signaling
T-cells
Clutches
Actins
T-Lymphocytes
1 Citation (Scopus)

An improved yeast surface display platform for the screening of nanobody immune libraries

Uchański, T., Zögg, T., Yin, J., Yuan, D., Wohlkönig, A., Fischer, B., Rosenbaum, D. M., Kobilka, B. K., Pardon, E. & Steyaert, J., Dec 1 2019, In : Scientific Reports. 9, 1, 382.

Research output: Contribution to journalArticle

Single-Domain Antibodies
Libraries
Yeasts
Acyl Carrier Protein
Antigens
4 Citations (Scopus)

Bacterial pseudokinase catalyzes protein polyglutamylation to inhibit the SidE-family ubiquitin ligases

Black, M. H., Osinski, A., Gradowski, M., Servage, K. A., Pawłowski, K., Tomchick, D. R. & Tagliabracci, V. S., May 24 2019, In : Science (New York, N.Y.). 364, 6442, p. 787-792 6 p.

Research output: Contribution to journalArticle

Ligases
Ubiquitin
Protein Kinases
Legionella
Calmodulin

Cryo-EM analyses reveal the common mechanism and diversification in the activation of RET by different ligands

Li, J., Shang, G., Chen, Y. J., Brautigam, C. A., Liou, J., Zhang, X. & Bai, X. C., Sep 2019, In : eLife. 8, e47650.

Research output: Contribution to journalArticle

Open Access
Receptor Protein-Tyrosine Kinases
Chemical activation
Ligands
Glial Cell Line-Derived Neurotrophic Factor
Phosphorylation
13 Citations (Scopus)

Cryo-EM structures of STING reveal its mechanism of activation by cyclic GMP–AMP

Shang, G., Zhang, C., Chen, Z., Bai, X. & Zhang, X., Mar 21 2019, In : Nature. 567, 7748, p. 389-393 5 p.

Research output: Contribution to journalLetter

Ligands
Chickens
Cryoelectron Microscopy
Interferon Type I
Endoplasmic Reticulum
7 Citations (Scopus)

Direct observation of individual tubulin dimers binding to growing microtubules

Mickolajczyk, K. J., Geyer, E. A., Kim, T., Rice, L. M. & Hancock, W. O., Apr 9 2019, In : Proceedings of the National Academy of Sciences of the United States of America. 116, 15, p. 7314-7322 9 p.

Research output: Contribution to journalArticle

Tubulin
Microtubules
Observation
Cell Division
Microscopy

ECOD: Identification of distant homology among multidomain and transmembrane domain proteins

Schaeffer, R. D., Kinch, L., Medvedev, K. E., Pei, J., Cheng, H. & Grishin, N. V., Jun 21 2019, In : BMC Molecular and Cell Biology. 20, 1, 18.

Research output: Contribution to journalArticle

Open Access
Leucine-tRNA Ligase
ATP-Binding Cassette Transporters
Dimerization
Anniversaries and Special Events
Leucine
1 Citation (Scopus)

Enhanced Sample Handling for Analytical Ultracentrifugation with 3D-Printed Centerpieces

To, S. C., Brautigam, C. A., Chaturvedi, S. K., Bollard, M. T., Krynitsky, J., Kakareka, J. W., Pohida, T. J., Zhao, H. & Schuck, P., May 7 2019, In : Analytical chemistry. 91, 9, p. 5866-5873 8 p.

Research output: Contribution to journalArticle

Sedimentation
Printing
Epoxy Resins
Aberrations
Durability

FISIK: Framework for the Inference of In Situ Interaction Kinetics from Single-Molecule Imaging Data

de Oliveira, L. R. & Jaqaman, K., Jan 1 2019, (Accepted/In press) In : Biophysical Journal.

Research output: Contribution to journalArticle

Theoretical Models
Biological Models
Single Molecule Imaging
Information Systems
Calibration

High-resolution cryo-EM structures of the E. Coli hemolysin ClyA oligomers

Peng, W., de Souza Santos, M., Li, Y., Tomchick, D. R. & Orth, K. A., May 1 2019, In : PloS one. 14, 5, e0213423.

Research output: Contribution to journalArticle

Open Access
hemolysins
Protein Subunits
protein subunits
Oligomers
Porins

Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone

Padavannil, A., Sarkar, P., Kim, S. J., Cagatay, T., Jiou, J., Brautigam, C. A., Tomchick, D. R., Sali, A., D'Arcy, S. & Chook, Y. M., Mar 11 2019, In : eLife. 8

Research output: Contribution to journalArticle

Open Access
Histone Chaperones
Karyopherins
Nucleosomes
Histones
DNA

Improved strategy for isoleucine 1H/13C methyl labeling in Pichia pastoris

Ali, R., Clark, L. D., Zahm, J. A., Lemoff, A., Ramesh, K., Rosenbaum, D. M. & Rosen, M. K., Jan 1 2019, (Accepted/In press) In : Journal of Biomolecular NMR.

Research output: Contribution to journalArticle

Open Access
Pichia
Isoleucine
Labeling
Proteins
Costs and Cost Analysis
1 Citation (Scopus)

Isotopic Labeling of Eukaryotic Membrane Proteins for NMR Studies of Interactions and Dynamics

Dikiy, I., Clark, L. D., Gardner, K. H. & Rosenbaum, D. M., Jan 1 2019, Methods in Enzymology. Wand, A. J. (ed.). Academic Press Inc., p. 37-65 29 p. (Methods in Enzymology; vol. 614).

Research output: Chapter in Book/Report/Conference proceedingChapter

Labeling
Membrane Proteins
Nuclear magnetic resonance
G-Protein-Coupled Receptors
Adenosine A2A Receptors
2 Citations (Scopus)

Kinesin-dependent transport of keratin filaments: A unified mechanism for intermediate filament transport

Robert, A., Tian, P., Adam, S. A., Kittisopikul, M., Jaqaman, K., Goldman, R. D. & Gelfand, V. I., Jan 1 2019, In : FASEB Journal. 33, 1, p. 388-399 12 p.

Research output: Contribution to journalArticle

Kinesin
Intermediate Filaments
Keratins
Clustered Regularly Interspaced Short Palindromic Repeats
Vimentin

Kinetochore-associated Stu2 promotes chromosome biorientation in vivo

Miller, M. P., Evans, R. K., Zelter, A., Geyer, E. A., MacCoss, M. J., Rice, L. M., Davis, T. N., Asbury, C. L. & Biggins, S., Oct 1 2019, In : PLoS genetics. 15, 10, p. e1008423

Research output: Contribution to journalArticle

Open Access
Kinetochores
kinetochores
chromosome
Chromosomes
chromosomes
1 Citation (Scopus)

Long-range, through-lattice coupling improves predictions of microtubule catastrophe

Kim, T. & Rice, L. M., Jan 1 2019, In : Molecular biology of the cell. 30, 12, p. 1451-1462 12 p.

Research output: Contribution to journalArticle

Open Access
Microtubules
Tubulin
Guanosine Triphosphate
Biochemistry
Chromosome Segregation
4 Citations (Scopus)

Membrane bridging by Munc13-1 is crucial for neurotransmitter release

Quade, B., Camacho, M., Zhao, X., Orlando, M., Trimbuch, T., Xu, J., Li, W., Nicastro, D., Rosenmund, C. & Rizo-Rey, J., Feb 28 2019, In : eLife. 8

Research output: Contribution to journalArticle

Open Access
Synaptic Vesicles
Neurotransmitter Agents
Synaptic Membranes
Cell membranes
Membranes
11 Citations (Scopus)

Molecular Discrimination between Two Conformations of Sphingomyelin in Plasma Membranes

Endapally, S., Frias, D., Grzemska, M., Gay, A., Tomchick, D. R. & Radhakrishnan, A., Feb 21 2019, In : Cell. 176, 5, p. 1040-1053.e17

Research output: Contribution to journalArticle

Sphingomyelins
Cell membranes
Conformations
Cholesterol
Cell Membrane
2 Citations (Scopus)

Multiple factors maintain assembled trans-SNARE complexes in the presence of NSF and αSNAP

Prinslow, E. A., Stepien, K. P., Pan, Y. Z., Xu, J. & Rizo-Rey, J., Jan 18 2019, In : eLife. 8

Research output: Contribution to journalArticle

Open Access
SNARE Proteins
Synaptotagmin I
Synaptic Vesicles
Synaptic Membranes
Cell membranes

Munc18-1 is crucial to overcome the inhibition of synaptic vesicle fusion by αSNAP

Stepien, K. P., Prinslow, E. A. & Rizo, J., Dec 1 2019, In : Nature communications. 10, 1, 4326.

Research output: Contribution to journalArticle

Open Access
Syntaxin 1
SNARE Proteins
Synaptic Vesicles
Liposomes
neurotransmitters

NMR and crystallographic structural studies of the extremely stable monomeric variant of human cystatin C with single amino acid substitution

Maszota-Zieleniak, M., Jurczak, P., Orlikowska, M., Zhukov, I., Borek, D., Otwinowski, Z., Skowron, P., Pietralik, Z., Kozak, M., Szymańska, A. & Rodziewicz-Motowidło, S., Jan 1 2019, In : FEBS Journal.

Research output: Contribution to journalArticle

Cystatin C
Amino Acid Substitution
Substitution reactions
Nuclear magnetic resonance
Amino Acids

Organization of Chromatin by Intrinsic and Regulated Phase Separation

Gibson, B. A., Doolittle, L. K., Schneider, M. W. G., Jensen, L. E., Gamarra, N., Henry, L., Gerlich, D. W., Redding, S. & Rosen, M. K., Oct 3 2019, In : Cell. 179, 2, p. 470-484.e21

Research output: Contribution to journalArticle

Acetylation
Nucleosomes
Epigenomics
Phase separation
Histones
3 Citations (Scopus)

Regulation of Transmembrane Signaling by Phase Separation

Case, L. B., Ditlev, J. A. & Rosen, M. K., May 6 2019, In : Annual Review of Biophysics. 48, p. 465-494 30 p.

Research output: Contribution to journalReview article

Wnt Receptors
Receptor Protein-Tyrosine Kinases
Cell Surface Receptors
Cell Adhesion
Phase separation
10 Citations (Scopus)

Stoichiometry controls activity of phase-separated clusters of actin signaling proteins

Case, L. B., Zhang, X., Ditlev, J. A. & Rosen, M. K., Mar 8 2019, In : Science (New York, N.Y.). 363, 6431, p. 1093-1097 5 p.

Research output: Contribution to journalArticle

Actin-Related Protein 2-3 Complex
Actins
Proteins
Membranes
Lipid Bilayers
2 Citations (Scopus)

Structural and functional characterization of an otopetrin family proton channel

Chen, Q., Zeng, W., She, J., Bai, X. & Jiang, Y., Apr 11 2019, In : eLife. 8

Research output: Contribution to journalArticle

Open Access
Constriction
Protons
Mutagenesis
Xenopus
Proteins
10 Citations (Scopus)

Structural basis of STING binding with and phosphorylation by TBK1

Zhang, C., Shang, G., Gui, X., Zhang, X., Bai, X. & Chen, Z., Jan 1 2019, In : Nature.

Research output: Contribution to journalLetter

Interferons
Phosphotransferases
Phosphorylation
Genes
Interferon Type I

Structural basis of the activation of type 1 insulin-like growth factor receptor

Li, J., Choi, E., Yu, H. & Bai, X. C., Dec 1 2019, In : Nature communications. 10, 1, 4567.

Research output: Contribution to journalArticle

Open Access
IGF Type 1 Receptor
insulin
Protein Subunits
Receptor Protein-Tyrosine Kinases
Dimerization

Structural Basis of TLR2/TLR1 Activation by the Synthetic Agonist Diprovocim

Su, L., Wang, Y., Wang, J., Mifune, Y., Morin, M. D., Jones, B. T., Moresco, E. M. Y., Boger, D. L., Beutler, B. A. & Zhang, H., Mar 28 2019, In : Journal of Medicinal Chemistry. 62, 6, p. 2938-2949 12 p.

Research output: Contribution to journalArticle

Ligands
Lipopeptides
Hydrogen Bonding
Hydrophobic and Hydrophilic Interactions
Melanoma
2 Citations (Scopus)

Structural Insight into DNA-Dependent Activation of Human Metalloprotease Spartan

Li, F., Raczynska, J. E., Chen, Z. & Yu, H., Mar 19 2019, In : Cell Reports. 26, 12, p. 3336-3346.e4

Research output: Contribution to journalArticle

Open Access
Metalloproteases
Single-Stranded DNA
Chemical activation
DNA
Progeria
2 Citations (Scopus)

Structural Mechanism of EMRE-Dependent Gating of the Human Mitochondrial Calcium Uniporter

Wang, Y., Nguyen, N. X., She, J., Zeng, W., Yang, Y., Bai, X. & Jiang, Y., May 16 2019, In : Cell. 177, 5, p. 1252-1261.e13

Research output: Contribution to journalArticle

Calcium
mitochondrial calcium uniporter
Mitochondria
Dimerization
Post Translational Protein Processing

Structural mechanisms of phospholipid activation of the human TPC2 channel

She, J., Zeng, W., Guo, J., Chen, Q., Bai, X. & Jiang, Y., Mar 12 2019, In : eLife. 8

Research output: Contribution to journalArticle

Open Access
Phospholipids
Chemical activation
Conformations
Ligands
Functional analysis
1 Citation (Scopus)
Open Access
Nuclear Export Signals
Cell Nucleus Active Transport
Binding Sites
Peptides
Proteins

Synaptic vesicle fusion: today and beyond

Brose, N., Brunger, A., Cafiso, D., Chapman, E. R., Diao, J., Hughson, F. M., Jackson, M. B., Jahn, R., Lindau, M., Ma, C., Rizo-Rey, J., Shin, Y. K., Söllner, T. H., Tamm, L., Yoon, T. Y. & Zhang, Y., Aug 1 2019, In : Nature structural & molecular biology. 26, 8, p. 663-668 6 p.

Research output: Contribution to journalComment/debate

Synaptic Vesicles
2018
12 Citations (Scopus)

Active nuclear import and passive nuclear export are the primary determinants of TDP-43 localization

Pinarbasi, E. S., Caǧatay, T., Fung, H. Y. J., Li, Y. C., Chook, Y. M. & Thomas, P. J., Dec 1 2018, In : Scientific Reports. 8, 1, 7083.

Research output: Contribution to journalArticle

Nuclear Export Signals
Cell Nucleus Active Transport
Nuclear Fusion
Karyopherins
RNA-Binding Proteins

A new look for the growing microtubule end?

Rice, L. M., Aug 1 2018, In : Journal of Cell Biology. 217, 8, p. 2609-2611 3 p.

Research output: Contribution to journalReview article

Microtubules
Electron Microscope Tomography
Three-Dimensional Imaging
In Vitro Techniques
4 Citations (Scopus)

An isolated CLASP TOG domain suppresses microtubule catastrophe and promotes rescue

Majumdar, S., Kim, T., Chen, Z., Munyoki, S., Tso, S. C., Brautigam, C. A. & Rice, L. M., Jun 1 2018, In : Molecular Biology of the Cell. 29, 11, p. 1359-1375 17 p.

Research output: Contribution to journalArticle

Microtubules
Tubulin
Proteins
Chromosome Segregation
Point Mutation
1 Citation (Scopus)

A sequence family database built on ECOD structural domains

Liao, Y., Schaeffer, R. D., Pei, J. & Grishin, N. V., Sep 1 2018, In : Bioinformatics. 34, 17, p. 2997-3003 7 p.

Research output: Contribution to journalArticle

Databases
Alignment
Selection Bias
Sequence Alignment
Seeds
4 Citations (Scopus)

Atypical activation of the G protein Gαq by the oncogenic mutation Q209P

Maziarz, M., Leyme, A., Marivin, A., Luebbers, A., Patel, P. P., Chen, Z., Sprang, S. R. & Garcia-Marcos, M., Jan 1 2018, In : Journal of Biological Chemistry. 293, 51, p. 19586-19599 14 p.

Research output: Contribution to journalArticle

Gq-G11 GTP-Binding Protein alpha Subunits
GTP Phosphohydrolases
Bearings (structural)
Chemical activation
RGS Proteins

Biophysical insights into a highly selective l-arginine-binding lipoprotein of a pathogenic treponeme

Deka, R. K., Liu, W. Z., Tso, S. C., Norgard, M. V. & Brautigam, C. A., Jan 1 2018, (Accepted/In press) In : Protein Science.

Research output: Contribution to journalArticle

Lipoproteins
Arginine
Treponema pallidum
Proteins
Carrier Proteins
1 Citation (Scopus)

Correlation of CRM1-NES affinity with nuclear export activity

Fu, S. C., Fung, H. Y. J., Caǧatay, T., Baumhardt, J. & Chook, Y. M., Aug 15 2018, In : Molecular Biology of the Cell. 29, 17, p. 2037-2044 8 p.

Research output: Contribution to journalArticle

Nuclear Export Signals
Cell Nucleus Active Transport
Peptides
Mutant Proteins
Protein Kinase Inhibitors
28 Citations (Scopus)

Cryo-EM structure of a fungal mitochondrial calcium uniporter

Nguyen, N. X., Armache, J. P., Lee, C., Yang, Y., Zeng, W., Mootha, V. K., Cheng, Y., Bai, X. C. & Jiang, Y., Jul 26 2018, In : Nature. 559, 7715, p. 570-574 5 p.

Research output: Contribution to journalArticle

Fungal Structures
Neosartorya
Cryoelectron Microscopy
Mitochondrial Membranes
Calcium Channels
28 Citations (Scopus)

Cryo-EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein

García-Nafría, J., Lee, Y., Bai, X., Carpenter, B. & Tate, C. G., May 4 2018, In : eLife. 7, e35946.

Research output: Contribution to journalArticle

Adenosine A2A Receptors
Heterotrimeric GTP-Binding Proteins
Single-Domain Antibodies
Crystal structure
Adenosine-5'-(N-ethylcarboxamide)
3 Citations (Scopus)

Crystal structure of native α-l-rhamnosidase from Aspergillus terreus

Pachl, P., Škerlová, J., Šimčíková, D., Kotik, M., Křenková, A., Mader, P., Brynda, J., Kapešová, J., Křen, V., Otwinowski, Z. & Řezáčová, P., Nov 1 2018, In : Acta Crystallographica Section D: Structural Biology. 74, 11, p. 1078-1084 7 p.

Research output: Contribution to journalArticle

Aspergillus
Catalytic Domain
Sulfur
X-Rays
Glucose
2 Citations (Scopus)

Crystal structure of native β-N-acetylhexosaminidase isolated from Aspergillus oryzae sheds light onto its substrate specificity, high stability, and regulation by propeptide

Škerlová, J., Bláha, J., Pachl, P., Hofbauerová, K., Kukačka, Z., Man, P., Pompach, P., Novák, P., Otwinowski, Z., Brynda, J., Vaněk, O. & Řezáčová, P., Jan 1 2018, (Accepted/In press) In : FEBS Journal.

Research output: Contribution to journalArticle

Aspergillus oryzae
Aspergillus
Substrate Specificity
Crystal structure
Substrates
2 Citations (Scopus)

Crystal structure of the human NK1 tachykinin receptor

Yin, J., Chapman, K., Clark, L. D., Shao, Z., Borek, D., Xu, Q., Wang, J. & Rosenbaum, D. M., Dec 26 2018, In : Proceedings of the National Academy of Sciences of the United States of America. 115, 52, p. 13264-13269 6 p.

Research output: Contribution to journalArticle

Tachykinin Receptors
aprepitant
L 760735
Vomiting
Depression
14 Citations (Scopus)

Crystal Structures of Human Orexin 2 Receptor Bound to the Subtype-Selective Antagonist EMPA

Suno, R., Kimura, K. T., Nakane, T., Yamashita, K., Wang, J., Fujiwara, T., Yamanaka, Y., Im, D., Horita, S., Tsujimoto, H., Tawaramoto, M. S., Hirokawa, T., Nango, E., Tono, K., Kameshima, T., Hatsui, T., Joti, Y., Yabashi, M., Shimamoto, K., Yamamoto, M. & 4 others, Rosenbaum, D. M., Iwata, S., Shimamura, T. & Kobayashi, T., Jan 2 2018, In : Structure. 26, 1, p. 7-19.e5

Research output: Contribution to journalArticle

Orexin Receptors
Synchrotrons
Molecular Dynamics Simulation
Dissociative Disorders
Crystallography
1 Citation (Scopus)

Crystal structures of the PH domains from Lbc family of RhoGEFs bound to activated RhoA GTPase

Chen, Z., Gutowski, S. & Sternweis, P. C., Apr 1 2018, In : Data in Brief. 17, p. 356-362 7 p.

Research output: Contribution to journalArticle

Rho Guanine Nucleotide Exchange Factors
GTP Phosphohydrolases
rho GTP-Binding Proteins
Manuscripts
Pleckstrin Homology Domains
1 Citation (Scopus)
Treponema pallidum
Carrier Proteins
Ligands
Glucose
Crystals
5 Citations (Scopus)

Design principles of a microtubule polymerase

Geyer, E. A., Miller, M. P., Brautigam, C. A., Biggins, S. & Rice, L. M., Jun 13 2018, In : eLife. 7, e34574.

Research output: Contribution to journalArticle

Tubulin
Microtubules
Mental Competency
Elongation
Organizations