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2020

A distinct inhibitory mechanism of the V-ATPase by Vibrio VopQ revealed by cryo-EM

Peng, W., Casey, A. K., Fernandez, J., Carpinone, E. M., Servage, K. A., Chen, Z., Li, Y., Tomchick, D. R., Starai, V. J. & Orth, K., Jun 1 2020, In : Nature Structural and Molecular Biology. 27, 6, p. 589-597 9 p.

Research output: Contribution to journalArticle

A Legionella effector kinase is activated by host inositol hexakisphosphate

Sreelatha, A., Nolan, C., Park, B. C., Pawłowski, K., Tomchick, D. R. & Tagliabracci, V. S., May 1 2020, In : Journal of Biological Chemistry. 295, 18, p. 6214-6224 11 p.

Research output: Contribution to journalArticle

Analysis of asymmetry in lipid and content mixing assays with reconstituted proteoliposomes containing the neuronal SNAREs

Pan, Y. Z., Liu, X. & Rizo, J., Dec 1 2020, In : Scientific reports. 10, 1, 2907.

Research output: Contribution to journalArticle

Open Access

A Phosphorylated Intermediate in the Activation of WNK Kinases

Akella, R., Drozdz, M. A., Humphreys, J. M., Jiou, J., Durbacz, M. Z., Mohammed, Z. J., He, H., Liwocha, J., Sekulski, K. & Goldsmith, E. J., May 12 2020, In : Biochemistry. 59, 18, p. 1747-1755 9 p.

Research output: Contribution to journalArticle

CLASPs at a glance

Lawrence, E. J., Zanic, M. & Rice, L. M., Apr 2020, In : Journal of cell science. 133, 8, jcs243097.

Research output: Contribution to journalArticle

Open Access

Cryo-EM structure of the PlexinC1/A39R complex reveals inter-domain interactions critical for ligand-induced activation

Kuo, Y. C., Chen, H., Shang, G., Uchikawa, E., Tian, H., Bai, X. C. & Zhang, X., Dec 1 2020, In : Nature communications. 11, 1, 1953.

Research output: Contribution to journalArticle

Open Access

Dynamin regulates the dynamics and mechanical strength of the actin cytoskeleton as a multifilament actin-bundling protein

Zhang, R., Lee, D. M., Jimah, J. R., Gerassimov, N., Yang, C., Kim, S., Luvsanjav, D., Winkelman, J., Mettlen, M., Abrams, M. E., Kalia, R., Keene, P., Pandey, P., Ravaux, B., Kim, J. H., Ditlev, J. A., Zhang, G., Rosen, M. K., Frost, A., Alto, N. M. & 5 others, Gardel, M., Schmid, S. L., Svitkina, T. M., Hinshaw, J. E. & Chen, E. H., Jun 1 2020, In : Nature cell biology. 22, 6, p. 674-688 15 p.

Research output: Contribution to journalArticle

Fast and Robust Quantification of Detergent Micellization Thermodynamics from Isothermal Titration Calorimetry

Tso, S. C., Mahler, F., Höring, J., Keller, S. & Brautigam, C. A., Jan 7 2020, In : Analytical chemistry. 92, 1, p. 1154-1161 8 p.

Research output: Contribution to journalArticle

1 Scopus citations

High-resolution cryo-EM reconstructions in the presence of substantial aberrations

Bromberg, R., Guo, Y., Borek, D. & Otwinowski, Z., May 1 2020, In : IUCrJ. 7, p. 445-452 8 p.

Research output: Contribution to journalArticle

Open Access

Insights into allosteric control of microtubule dynamics from a buried β-tubulin mutation that causes faster growth and slower shrinkage

Ye, X., Kim, T., Geyer, E. A. & Rice, L. M., Jun 1 2020, In : Protein Science. 29, 6, p. 1429-1439 11 p.

Research output: Contribution to journalArticle

Lead optimization of a pyrrole-based dihydroorotate dehydrogenase inhibitor series for the treatment of malaria

Kokkonda, S., Deng, X., White, K. L., El Mazouni, F., White, J., Shackleford, D. M., Katneni, K., Chiu, F. C. K., Barker, H., Mclaren, J., Crighton, E., Chen, G., Angulo-Barturen, I., Jimenez-Diaz, M. B., Ferrer, S., Huertas-Valentin, L., Martinez-Martinez, M. S., Lafuente-Monasterio, M. J., Chittimalla, R., Shahi, S. P. & 9 others, Wittlin, S., Waterson, D., Burrows, J. N., Matthews, D., Tomchick, D., Rathod, P. K., Palmer, M. J., Charman, S. A. & Phillips, M. A., May 14 2020, In : Journal of Medicinal Chemistry. 63, 9, p. 4929-4956 28 p.

Research output: Contribution to journalArticle

Mutation severity spectrum of rare alleles in the human genome is predictive of disease type

Pei, J., Kinch, L. N., Otwinowski, Z. & Grishin, N. V., May 2020, In : PLoS Computational Biology. 16, 5, e1007775.

Research output: Contribution to journalArticle

Open Access

Nucleating a Microtubule: Put a γ-Tubulin Ring on It

Gangadharan, B. & Rice, L. M., Jun 8 2020, In : Developmental cell. 53, 5, p. 495-497 3 p.

Research output: Contribution to journalArticle

Re-examining how Munc13-1 facilitates opening of syntaxin-1

Magdziarek, M., Bolembach, A. A., Stepien, K. P., Quade, B., Liu, X. & Rizo, J., Jun 1 2020, In : Protein Science. 29, 6, p. 1440-1458 19 p.

Research output: Contribution to journalArticle

Structural and mechanistic insights into secretagogin-mediated exocytosis

Qin, J., Liu, Q., Liu, Z., Pan, Y. Z., Sifuentes-Dominguez, L., Stepien, K. P., Wang, Y., Tu, Y., Tan, S., Wang, Y., Sun, Q., Mo, X., Rizo, J., Burstein, E. & Jia, D., Mar 24 2020, In : Proceedings of the National Academy of Sciences of the United States of America. 117, 12, p. 6559-6570 12 p.

Research output: Contribution to journalArticle

Structure of a D2 dopamine receptor–G-protein complex in a lipid membrane

Yin, J., Chen, K. Y. M., Clark, M. J., Hijazi, M., Kumari, P., Bai, X., Sunahara, R. K., Barth, P. & Rosenbaum, D. M., Jan 1 2020, (Accepted/In press) In : Nature.

Research output: Contribution to journalArticle

Synaptotagmin-1 and Doc2b Exhibit Distinct Membrane-Remodeling Mechanisms

Sorkin, R., Marchetti, M., Logtenberg, E., Piontek, M. C., Kerklingh, E., Brand, G., Voleti, R., Rizo, J., Roos, W. H., Groffen, A. J. & Wuite, G. J. L., Feb 4 2020, In : Biophysical journal. 118, 3, p. 643-656 14 p.

Research output: Contribution to journalArticle

2019

A Bacterial Effector Mimics a Host HSP90 Client to Undermine Immunity

Lopez, V. A., Park, B. C., Nowak, D., Sreelatha, A., Zembek, P., Fernandez, J., Servage, K. A., Gradowski, M., Hennig, J., Tomchick, D. R., Pawłowski, K., Krzymowska, M. & Tagliabracci, V. S., Sep 19 2019, In : Cell. 179, 1, p. 205-218.e21

Research output: Contribution to journalArticle

6 Scopus citations

A composition-dependent molecular clutch between T cell signaling condensates and actin

Ditlev, J. A., Vega, A. R., Köster, D. V., Su, X., Tani, T., Lakoduk, A. M., Vale, R. D., Mayor, S., Jaqaman, K. & Rosen, M. K., Jul 2019, In : eLife. 8, e42695.

Research output: Contribution to journalArticle

Open Access
8 Scopus citations

Activation mechanism of the insulin receptor revealed by cryo-EM structure of the fully liganded receptor-ligand complex

Uchikawa, E., Choi, E., Shang, G., Yu, H. & Xiao-Chen, B., Aug 2019, In : eLife. 8, e48630.

Research output: Contribution to journalArticle

Open Access
11 Scopus citations

An improved yeast surface display platform for the screening of nanobody immune libraries

Uchański, T., Zögg, T., Yin, J., Yuan, D., Wohlkönig, A., Fischer, B., Rosenbaum, D. M., Kobilka, B. K., Pardon, E. & Steyaert, J., Dec 1 2019, In : Scientific reports. 9, 1, 382.

Research output: Contribution to journalArticle

5 Scopus citations

Bacterial pseudokinase catalyzes protein polyglutamylation to inhibit the SidE-family ubiquitin ligases

Black, M. H., Osinski, A., Gradowski, M., Servage, K. A., Pawłowski, K., Tomchick, D. R. & Tagliabracci, V. S., May 24 2019, In : Science (New York, N.Y.). 364, 6442, p. 787-792 6 p.

Research output: Contribution to journalArticle

16 Scopus citations

Cryo-EM analyses reveal the common mechanism and diversification in the activation of RET by different ligands

Li, J., Shang, G., Chen, Y. J., Brautigam, C. A., Liou, J., Zhang, X. & Bai, X. C., Sep 2019, In : eLife. 8, e47650.

Research output: Contribution to journalArticle

Open Access
2 Scopus citations

Cryo-EM structures of the human cation-chloride cotransporter KCC1

Liu, S., Chang, S., Han, B., Xu, L., Zhang, M., Zhao, C., Yang, W., Wang, F., Li, J., Delpire, E., Ye, S., Bai, X. C. & Guo, J., Oct 25 2019, In : Science. 366, 6464, p. 505-508 4 p.

Research output: Contribution to journalArticle

5 Scopus citations

Direct observation of individual tubulin dimers binding to growing microtubules

Mickolajczyk, K. J., Geyer, E. A., Kim, T., Rice, L. M. & Hancock, W. O., Apr 9 2019, In : Proceedings of the National Academy of Sciences of the United States of America. 116, 15, p. 7314-7322 9 p.

Research output: Contribution to journalArticle

12 Scopus citations

ECOD: Identification of distant homology among multidomain and transmembrane domain proteins

Schaeffer, R. D., Kinch, L., Medvedev, K. E., Pei, J., Cheng, H. & Grishin, N., Jun 21 2019, In : BMC Molecular and Cell Biology. 20, 1, 18.

Research output: Contribution to journalArticle

Open Access
1 Scopus citations

Enhanced Sample Handling for Analytical Ultracentrifugation with 3D-Printed Centerpieces

To, S. C., Brautigam, C. A., Chaturvedi, S. K., Bollard, M. T., Krynitsky, J., Kakareka, J. W., Pohida, T. J., Zhao, H. & Schuck, P., May 7 2019, In : Analytical chemistry. 91, 9, p. 5866-5873 8 p.

Research output: Contribution to journalArticle

2 Scopus citations

FISIK: Framework for the Inference of In Situ Interaction Kinetics from Single-Molecule Imaging Data

de Oliveira, L. R. & Jaqaman, K., Jan 1 2019, (Accepted/In press) In : Biophysical Journal.

Research output: Contribution to journalArticle

Functional analysis of rossmann-like domains reveals convergent evolution of topology and reaction pathways

Medvedevid, K. E., Kinch, L. N., Schaeffer, R. D. & Grishin, N. V., Dec 23 2019, In : PLoS Computational Biology. 15, 12, e1007569.

Research output: Contribution to journalArticle

Open Access

High-resolution cryo-EM structures of the E. Coli hemolysin ClyA oligomers

Peng, W., de Souza Santos, M., Li, Y., Tomchick, D. R. & Orth, K., May 2019, In : PloS one. 14, 5, e0213423.

Research output: Contribution to journalArticle

Open Access
2 Scopus citations

Histone lysine demethylase KDM4B regulates the alternative splicing of the androgen receptor in response to androgen deprivation

Duan, L., Chen, Z., Lu, J., Liang, Y., Wang, M., Roggero, C. M., Zhang, Q. J., Gao, J., Fang, Y., Cao, J., Lu, J., Zhao, H., Dang, A., Pong, R. C., Hernandez, E., Chang, C. M., Hoang, D. T., Ahn, J. M., Xiao, G., Wang, R. T. & 6 others, Yu, K. J., Kapur, P., Rizo, J., Hsieh, J. T., Luo, J. & Liu, Z. P., Dec 16 2019, In : Nucleic acids research. 47, 22, p. 11623-11636 14 p.

Research output: Contribution to journalArticle

Open Access

Identification and Mechanistic Understanding of Dihydroorotate Dehydrogenase Point Mutations in Plasmodium falciparum that Confer in Vitro Resistance to the Clinical Candidate DSM265

White, J., Dhingra, S. K., Deng, X., El Mazouni, F., Lee, M. C. S., Afanador, G. A., Lawong, A., Tomchick, D. R., Ng, C. L., Bath, J., Rathod, P. K., Fidock, D. A. & Phillips, M. A., Nov 1 2019, In : ACS infectious diseases. 5, 1, p. 90-101 12 p.

Research output: Contribution to journalArticle

6 Scopus citations

Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone

Padavannil, A., Sarkar, P., Kim, S. J., Cagatay, T., Jiou, J., Brautigam, C. A., Tomchick, D. R., Sali, A., D’Arcy, S. & Chook, Y. M., Mar 2019, In : eLife. 8, e43630.

Research output: Contribution to journalArticle

Open Access
4 Scopus citations

Improved strategy for isoleucine 1H/13C methyl labeling in Pichia pastoris

Ali, R., Clark, L. D., Zahm, J. A., Lemoff, A., Ramesh, K., Rosenbaum, D. M. & Rosen, M. K., Dec 1 2019, In : Journal of biomolecular NMR. 73, 12, p. 687-697 11 p.

Research output: Contribution to journalArticle

Open Access
1 Scopus citations

Kinesin-dependent transport of keratin filaments: A unified mechanism for intermediate filament transport

Robert, A., Tian, P., Adam, S. A., Kittisopikul, M., Jaqaman, K., Goldman, R. D. & Gelfand, V. I., Jan 1 2019, In : FASEB Journal. 33, 1, p. 388-399 12 p.

Research output: Contribution to journalArticle

3 Scopus citations

Kinetochore-associated Stu2 promotes chromosome biorientation in vivo

Miller, M. P., Evans, R. K., Zelter, A., Geyer, E. A., MacCoss, M. J., Rice, L. M., Davis, T. N., Asbury, C. L. & Biggins, S., Oct 1 2019, In : PLoS genetics. 15, 10, p. e1008423

Research output: Contribution to journalArticle

Open Access

Long-range, through-lattice coupling improves predictions of microtubule catastrophe

Kim, T. & Rice, L. M., Jan 1 2019, In : Molecular biology of the cell. 30, 12, p. 1451-1462 12 p.

Research output: Contribution to journalArticle

Open Access
2 Scopus citations

Membrane bridging by Munc13-1 is crucial for neurotransmitter release

Quade, B., Camacho, M., Zhao, X., Orlando, M., Trimbuch, T., Xu, J., Li, W., Nicastro, D., Rosenmund, C. & Rizo-Rey, J., Feb 28 2019, In : eLife. 8

Research output: Contribution to journalArticle

Open Access
7 Scopus citations

Molecular Discrimination between Two Conformations of Sphingomyelin in Plasma Membranes

Endapally, S., Frias, D., Grzemska, M., Gay, A., Tomchick, D. R. & Radhakrishnan, A., Feb 21 2019, In : Cell. 176, 5, p. 1040-1053.e17

Research output: Contribution to journalArticle

21 Scopus citations

Multiple factors maintain assembled trans-SNARE complexes in the presence of NSF and αSNAP

Prinslow, E. A., Stepien, K. P., Pan, Y. Z., Xu, J. & Rizo, J., Jan 18 2019, In : eLife. 8

Research output: Contribution to journalArticle

Open Access
9 Scopus citations

Munc18-1 is crucial to overcome the inhibition of synaptic vesicle fusion by αSNAP

Stepien, K. P., Prinslow, E. A. & Rizo, J., Dec 1 2019, In : Nature communications. 10, 1, 4326.

Research output: Contribution to journalArticle

Open Access
3 Scopus citations

NMR and crystallographic structural studies of the extremely stable monomeric variant of human cystatin C with single amino acid substitution

Maszota-Zieleniak, M., Jurczak, P., Orlikowska, M., Zhukov, I., Borek, D., Otwinowski, Z., Skowron, P., Pietralik, Z., Kozak, M., Szymańska, A. & Rodziewicz-Motowidło, S., Jan 1 2019, In : FEBS Journal.

Research output: Contribution to journalArticle

Organization of Chromatin by Intrinsic and Regulated Phase Separation

Gibson, B. A., Doolittle, L. K., Schneider, M. W. G., Jensen, L. E., Gamarra, N., Henry, L., Gerlich, D. W., Redding, S. & Rosen, M. K., Oct 3 2019, In : Cell. 179, 2, p. 470-484.e21

Research output: Contribution to journalArticle

40 Scopus citations

Phosphorylation of nephrin induces phase separated domains that move through actomyosin contraction

Kim, S., Kalappurakkal, J. M., Mayor, S. & Rosen, M. K., Nov 15 2019, In : Molecular biology of the cell. 30, 24, p. 2996-3012 17 p.

Research output: Contribution to journalArticle

Open Access

Stoichiometry controls activity of phase-separated clusters of actin signaling proteins

Case, L. B., Zhang, X., Ditlev, J. A. & Rosen, M. K., Mar 8 2019, In : Science (New York, N.Y.). 363, 6431, p. 1093-1097 5 p.

Research output: Contribution to journalArticle

30 Scopus citations

Structural and functional characterization of an otopetrin family proton channel

Chen, Q., Zeng, W., She, J., Bai, X. & Jiang, Y., Apr 11 2019, In : eLife. 8

Research output: Contribution to journalArticle

Open Access
4 Scopus citations

Structural basis of STING binding with and phosphorylation by TBK1

Zhang, C., Shang, G., Gui, X., Zhang, X., Bai, X. C. & Chen, Z. J., Mar 21 2019, In : Nature. 567, 7748, p. 394-398 5 p.

Research output: Contribution to journalArticle

41 Scopus citations

Structural basis of the activation of type 1 insulin-like growth factor receptor

Li, J., Choi, E., Yu, H. & Bai, X. C., Dec 1 2019, In : Nature communications. 10, 1, 4567.

Research output: Contribution to journalArticle

Open Access
6 Scopus citations

Structural Basis of TLR2/TLR1 Activation by the Synthetic Agonist Diprovocim

Su, L., Wang, Y., Wang, J., Mifune, Y., Morin, M. D., Jones, B. T., Moresco, E. M. Y., Boger, D. L., Beutler, B. & Zhang, H., Mar 28 2019, In : Journal of Medicinal Chemistry. 62, 6, p. 2938-2949 12 p.

Research output: Contribution to journalArticle

2 Scopus citations