Cell Biology - Division U

Research Output

2019

CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation

Tuladhar, R., Yeu, Y., Tyler Piazza, J., Tan, Z., Rene Clemenceau, J., Wu, X., Barrett, Q., Herbert, J., Mathews, D. H., Kim, J., Hyun Hwang, T. & Lum, L., Dec 1 2019, In : Nature communications. 10, 1, 4056.

Research output: Contribution to journalArticle

Open Access
6 Scopus citations

Stereoselective fatty acylation is essential for the release of lipidated WNT proteins from the acyltransferase Porcupine (PORCN)

Tuladhar, R., Yarravarapu, N., Ma, Y., Zhang, C., Herbert, J., Kim, J., Chen, C. & Lum, L., Apr 19 2019, In : Journal of Biological Chemistry. 294, 16, p. 6273-6282 10 p.

Research output: Contribution to journalArticle

2018

A synthetic combinatorial approach to disabling deviant Hedgehog signaling

Fan, C. W., Yarravarapu, N., Shi, H., Kulak, O., Kim, J., Chen, C. & Lum, L., Dec 1 2018, In : Scientific Reports. 8, 1, 1133.

Research output: Contribution to journalArticle

Chemical Modulation of WNT Signaling in Cancer

Zhang, L. S. & Lum, L., Jan 1 2018, WNT Signaling in Health and Disease. Elsevier B.V., p. 245-269 25 p. (Progress in Molecular Biology and Translational Science; vol. 153).

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

EGF receptor and mTORC1 are novel therapeutic targets in nonseminomatous germ cell tumors

Chen, K., Fustino, N. J., Shukla, A. A., Stroup, E. K., Budhipramono, A., Ateek, C., Stuart, S. H., Yamaguchi, K., Kapur, P., Frazier, A. L., Lum, L., Looijenga, L. H. J., Laetsch, T. W., Rakheja, D. & Amatruda, J. F., May 1 2018, In : Molecular Cancer Therapeutics. 17, 5, p. 1079-1089 11 p.

Research output: Contribution to journalArticle

2 Scopus citations

Installation of a cancer promoting WNT/SIX1 signaling axis by the oncofusion protein MLL-AF9

Zhang, L. S., Kang, X., Lu, J., Zhang, Y., Wu, X., Wu, G., Zheng, J., Tuladhar, R., Shi, H., Wang, Q., Morlock, L., Yao, H., Huang, J-S., Maire, P., Kim, J., Williams, N. S., Xu, J., Chen, C., Zhang, C. & Lum, L., Jan 1 2018, (Accepted/In press) In : EBioMedicine.

Research output: Contribution to journalArticle

2 Scopus citations

Regulation of tankyrase activity by a catalytic domain dimer interface

Fan, C., Yarravarapu, N., Chen, H., Kulak, O., Dasari, P., Herbert, J., Yamaguchi, K., Lum, L. & Zhang, X., Jan 1 2018, (Accepted/In press) In : Biochemical and Biophysical Research Communications.

Research output: Contribution to journalArticle

5 Scopus citations
2017

Blockade to pathological remodeling of infarcted heart tissue using a porcupine antagonist

Moon, J., Zhou, H., Zhang, L. S., Tan, W., Liu, Y., Zhang, S., Morlock, L. K., Bao, X., Palecek, S. P., Feng, J. Q., Williams, N. S., Amatruda, J. F., Olson, E. N., Bassel-Duby, R. & Lum, L., Feb 14 2017, In : Proceedings of the National Academy of Sciences of the United States of America. 114, 7, p. 1649-1654 6 p.

Research output: Contribution to journalArticle

22 Scopus citations

Extra-mitochondrial prosurvival BCL-2 proteins regulate gene transcription by inhibiting the SUFU tumour suppressor

Wu, X., Zhang, L. S., Toombs, J., Kuo, Y. C., Piazza, J. T., Tuladhar, R., Barrett, Q., Fan, C. W., Zhang, X., Walensky, L. D., Kool, M., Cheng, S. Y., Brekken, R., Opferman, J. T., Green, D. R., Moldoveanu, T. & Lum, L., Sep 29 2017, In : Nature Cell Biology. 19, 10, p. 1226-1236 11 p.

Research output: Contribution to journalArticle

15 Scopus citations
2016

Development of a triazole class of highly potent Porcn inhibitors

You, L., Zhang, C., Yarravarapu, N., Morlock, L., Wang, X., Zhang, L., Williams, N. S., Lum, L. & Chen, C., Dec 15 2016, In : Bioorganic and Medicinal Chemistry Letters. 26, 24, p. 5891-5895 5 p.

Research output: Contribution to journalArticle

7 Scopus citations

Monitoring wnt protein acylation using an in vitro cyclo- addition reaction

Tuladhar, R., Yarravarapu, N. & Lum, L., Jan 1 2016, Methods in Molecular Biology. Humana Press Inc., p. 11-16 6 p. (Methods in Molecular Biology; vol. 1481).

Research output: Chapter in Book/Report/Conference proceedingChapter

2015
13 Scopus citations

Disruption of Wnt/β-catenin signaling and telomeric shortening are inextricable consequences of tankyrase inhibition in human cells

Kulak, O., Chen, H., Holohan, B., Wu, X., He, H., Borek, D., Otwinowski, Z., Yamaguchi, K., Garofalo, L. A., Ma, Z., Wright, W., Chen, C., Shay, J. W., Zhang, X. & Lum, L., 2015, In : Molecular and Cellular Biology. 35, 14, p. 2425-2435 11 p.

Research output: Contribution to journalArticle

35 Scopus citations

Erratum: The hedgehog pathway effector smoothened exhibits signaling competency in the absence of ciliary accumulation (Chemistry and Biology (2014) 21 (1680-689))

Fan, C. W., Chen, B., Franco, I., Lu, J., Shi, H., Wei, S., Wang, C., Wu, X., Tang, W., Roth, M. G., Williams, N. S., Hirsch, E., Chen, C. & Lum, L., Apr 23 2015, In : Chemistry and Biology. 22, 4, p. 559 1 p.

Research output: Contribution to journalArticle

Fatty acyl donor selectivity in membrane bound o-acyltransferases and communal cell fate decision-making

Tuladhar, R. & Lum, L., Apr 1 2015, In : Biochemical Society Transactions. 43, p. 235-239 5 p.

Research output: Contribution to journalArticle

10 Scopus citations

Identification of therapeutic small-molecule leads in cultured cells using multiplexed pathway reporter readouts

Kulak, O., Yamaguchi, K. & Lum, L., Jan 1 2015, In : Methods in Molecular Biology. 1263, p. 3-16 14 p.

Research output: Contribution to journalArticle

1 Scopus citations

Role of the exocyst complex component Sec6/8 in genomic stability

Torres, M. J., Pandita, R. K., Kulak, O., Kumar, R., Formstecher, E., Horikoshi, N., Mujoo, K., Hunt, C. R., Zhao, Y., Lum, L., Zaman, A., Yeaman, C., White, M. A. & Pandita, T. K., Jan 1 2015, In : Molecular and cellular biology. 35, 21, p. 3633-3645 13 p.

Research output: Contribution to journalArticle

6 Scopus citations
2014

Computational detection and suppression of sequence-specific off-target phenotypes from whole genome RNAi screens

Zhong, R., Kim, J., Kim, H. S., Kim, M., Lum, L., Levine, B., Xiao, G., White, M. A. & Xie, Y., 2014, In : Nucleic acids research. 42, 13, p. 8214-8222 9 p.

Research output: Contribution to journalArticle

17 Scopus citations

KIF14 promotes AKT phosphorylation and contributes to chemoresistance in triple-negative breast cancer

Singel, S. M., Cornelius, C., Zaganjor, E., Batten, K., Sarode, V. R., Buckley, D. L., Peng, Y., John, G. B., Li, H. C., Sadeghi, N., Wright, W. E., Lum, L., Corson, T. W. & Shay, J. W., Mar 2014, In : Neoplasia (United States). 16, 3, p. 247-256.e2 e2.

Research output: Contribution to journalArticle

40 Scopus citations

The hedgehog pathway effector smoothened exhibits signaling competency in the absence of ciliary accumulation

Fan, C. W., Chen, B., Franco, I., Lu, J., Shi, H., Wei, S., Wang, C., Wu, X., Tang, W., Roth, M. G., Williams, N. S., Hirsch, E., Chen, C. & Lum, L., Dec 18 2014, In : Chemistry and Biology. 21, 12, p. 1680-1689 10 p.

Research output: Contribution to journalArticle

12 Scopus citations
2013
5 Scopus citations

A targeted RNAi screen of the breast cancer genome identifies KIF14 and TLN1 as genes that modulate docetaxel chemosensitivity in triple-negative breast cancer

Singel, S. M., Cornelius, C., Batten, K., Fasciani, G., Wright, W. E., Lum, L. & Shay, J. W., Apr 15 2013, In : Clinical Cancer Research. 19, 8, p. 2061-2070 10 p.

Research output: Contribution to journalArticle

37 Scopus citations

Erratum: Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation (Nature Cell Biology (2013) 15 (1035-1044))

Das, A., Tanigawa, S., Karner, C. M., Xin, M., Lum, L., Chen, C., Olson, E. N., Perantoni, A. O. & Carroll, T. J., Oct 1 2013, In : Nature cell biology. 15, 10, 1 p.

Research output: Contribution to journalComment/debate

1 Scopus citations

Signaling: Making a leaner Hedgehog

Fan, C. W., Tuladhar, R. & Lum, L., Apr 1 2013, In : Nature chemical biology. 9, 4, p. 217-218 2 p.

Research output: Contribution to journalComment/debate

Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation

Das, A., Tanigawa, S., Karner, C. M., Xin, M., Lum, L., Chen, C., Olson, E. N., Perantoni, A. O. & Carroll, T. J., Sep 1 2013, In : Nature cell biology. 15, 9, p. 1035-1044 10 p.

Research output: Contribution to journalArticle

129 Scopus citations

The development of highly potent inhibitors for porcupine

Wang, X., Moon, J., Dodge, M. E., Pan, X., Zhang, L., Hanson, J. M., Tuladhar, R., Ma, Z., Shi, H., Williams, N. S., Amatruda, J. F., Carroll, T. J., Lum, L. & Chen, C., Mar 28 2013, In : Journal of Medicinal Chemistry. 56, 6, p. 2700-2704 5 p.

Research output: Contribution to journalArticle

46 Scopus citations
2012

Diverse chemical scaffolds support direct inhibition of the membrane-bound O-acyltransferase porcupine

Dodge, M. E., Moon, J., Tuladhar, R., Lu, J., Jacob, L. S., Zhang, L. S., Shi, H., Wang, X., Moro, E., Mongera, A., Argenton, F., Karner, C. M., Carroll, T. J., Chen, C., Amatruda, J. F. & Lum, L., Jun 29 2012, In : Journal of Biological Chemistry. 287, 27, p. 23246-23254 9 p.

Research output: Contribution to journalArticle

54 Scopus citations

In vivo Wnt signaling tracing through a transgenic biosensor fish reveals novel activity domains

Moro, E., Ozhan-Kizil, G., Mongera, A., Beis, D., Wierzbicki, C., Young, R. M., Bournele, D., Domenichini, A., Valdivia, L. E., Lum, L., Chen, C., Amatruda, J. F., Tiso, N., Weidinger, G. & Argenton, F., Jun 15 2012, In : Developmental Biology. 366, 2, p. 327-340 14 p.

Research output: Contribution to journalArticle

114 Scopus citations

Mechanistic insight into Myc stabilization in breast cancer involving aberrant Axin1 expression

Zhang, X., Farrell, A. S., Daniel, C. J., Arnold, H., Scanlan, C., Laraway, B. J., Janghorban, M., Lum, L., Chen, D., Troxell, M. & Sears, R., Feb 21 2012, In : Proceedings of the National Academy of Sciences of the United States of America. 109, 8, p. 2790-2795 6 p.

Research output: Contribution to journalArticle

47 Scopus citations

The chemistry and biology of nakiterpiosin - C-nor-D-homosteroids

Gao, S., Wang, Q., Wang, G., Lomenick, B., Liu, J., Fan, C. W., Deng, L. W., Huang, J., Lum, L. & Chen, C., Dec 1 2012, In : Synlett. 23, 16, p. 2298-2310 13 p., ST-2012-A0436-A.

Research output: Contribution to journalArticle

5 Scopus citations

The unusual case of porcupine

Lum, L. & Clevers, H., Aug 24 2012, In : Science. 337, 6097, p. 922-923 2 p.

Research output: Contribution to journalReview article

51 Scopus citations
2011

Canonical Wnt9b signaling balances progenitor cell expansion and differentiation during kidney development

Karner, C. M., Das, A., Ma, Z., Self, M., Chen, C., Lum, L., Oliver, G. & Carroll, T. J., Apr 1 2011, In : Development. 138, 7, p. 1247-1257 11 p.

Research output: Contribution to journalArticle

163 Scopus citations
40 Scopus citations

Genome-wide RNAi screen reveals disease-associated genes that are common to Hedgehog and Wnt signaling

Jacob, L. S., Wu, X., Dodge, M. E., Fan, C. W., Kulak, O., Chen, B., Tang, W., Wang, B., Amatruda, J. F. & Lum, L., Jan 25 2011, In : Science Signaling. 4, 157, ra4.

Research output: Contribution to journalArticle

68 Scopus citations

Small molecule Wnt inhibitors enhance the efficiency of BMP-4-directed cardiac differentiation of human pluripotent stem cells

Ren, Y., Lee, M. Y., Schliffke, S., Paavola, J., Amos, P. J., Ge, X., Ye, M., Zhu, S., Senyei, G., Lum, L., Ehrlich, B. E. & Qyang, Y., Sep 1 2011, In : Journal of Molecular and Cellular Cardiology. 51, 3, p. 280-287 8 p.

Research output: Contribution to journalArticle

97 Scopus citations
2010

Chemical and biological studies of nakiterpiosin and nakiterpiosinone

Gao, S., Wang, Q., Huang, L. J. S., Lum, L. & Chen, C., Jan 13 2010, In : Journal of the American Chemical Society. 132, 1, p. 371-383 13 p.

Research output: Contribution to journalArticle

49 Scopus citations

Tankyrase is necessary for canonical Wnt signaling during kidney development

Karner, C. M., Merkel, C. E., Dodge, M., Ma, Z., Lu, J., Chen, C., Lum, L. & Carroll, T. J., Jul 2010, In : Developmental Dynamics. 239, 7, p. 2014-2023 10 p.

Research output: Contribution to journalArticle

33 Scopus citations
2009

Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer

Chen, B., Dodge, M. E., Tang, W., Lu, J., Ma, Z., Fan, C. W., Wei, S., Hao, W., Kilgore, J., Williams, N. S., Roth, M. G., Amatruda, J. F., Chen, C. & Lum, L., Feb 6 2009, In : Nature chemical biology. 5, 2, p. 100-107 8 p.

Research output: Contribution to journalArticle

918 Scopus citations

Structure-activity relationship studies of small-molecule inhibitors of Wnt response

Lu, J., Ma, Z., Hsieh, J. C., Fan, C. W., Chen, B., Longgood, J. C., Williams, N. S., Amatruda, J. F., Lum, L. & Chen, C., Jul 15 2009, In : Bioorganic and Medicinal Chemistry Letters. 19, 14, p. 3825-3827 3 p.

Research output: Contribution to journalArticle

90 Scopus citations
2008

A genome-wide RNAi screen for Wnt/β-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer

Tang, W., Dodge, M., Gundapaneni, D., Michnoff, C., Roth, M. & Lum, L., Jul 15 2008, In : Proceedings of the National Academy of Sciences of the United States of America. 105, 28, p. 9697-9702 6 p.

Research output: Contribution to journalArticle

125 Scopus citations
2007

Deconstructing the hedgehog pathway in development and disease

Jacob, L. & Lum, L., Oct 5 2007, In : Science. 318, 5847, p. 66-68 3 p.

Research output: Contribution to journalShort survey

51 Scopus citations

Hedgehog signaling pathway.

Jacob, L. & Lum, L., Oct 9 2007, In : Science's STKE : signal transduction knowledge environment. 2007, 407, p. cm6

Research output: Contribution to journalReview article

10 Scopus citations

Hedgehog signaling pathway in Drosophila.

Jacob, L. & Lum, L., Oct 9 2007, In : Science's STKE : signal transduction knowledge environment. 2007, 407, p. cm7

Research output: Contribution to journalReview article

6 Scopus citations
2006

A genomewide screen for components of the RNAi pathway in Drosophila cultured cells

Dorner, S., Lum, L., Kim, M., Paro, R., Beachy, P. A. & Green, R., Aug 8 2006, In : Proceedings of the National Academy of Sciences of the United States of America. 103, 32, p. 11880-11885 6 p.

Research output: Contribution to journalArticle

44 Scopus citations

Minimizing the risk of reporting false positives in large-scale RNAi screens

Echeverri, C. J., Beachy, P. A., Baum, B., Boutros, M., Buchholz, F., Chanda, S. K., Downward, J., Ellenberg, J., Fraser, A. G., Hacohen, N., Hahn, W. C., Jackson, A. L., Kiger, A., Linsley, P. S., Lum, L., Ma, Y., Mathey-Prévôt, B., Root, D. E., Sabatini, D. M., Taipale, J. & 2 others, Perrimon, N. & Bernards, R., Oct 1 2006, In : Nature methods. 3, 10, p. 777-779 3 p.

Research output: Contribution to journalArticle

339 Scopus citations

Prevalence of off-target effects in Drosophila RNA interference screens

Ma, Y., Creanga, A., Lum, L. & Beachy, P. A., Sep 21 2006, In : Nature. 443, 7109, p. 359-363 5 p.

Research output: Contribution to journalArticle

218 Scopus citations

The Ihog Cell-Surface Proteins Bind Hedgehog and Mediate Pathway Activation

Yao, S., Lum, L. & Beachy, P., Apr 21 2006, In : Cell. 125, 2, p. 343-357 15 p.

Research output: Contribution to journalArticle

149 Scopus citations
2004

Evidence for a Critical Role of the Tumor Necrosis Factor α Convertase (TACE) in Ectodomain Shedding of the p75 Neurotrophin Receptor (p75NTR)

Weskamp, G., Schlöndorff, J., Lum, L., Becherer, J. D., Kim, T. W., Saftig, P., Hartmann, D., Murphy, G. & Blobel, C. P., Feb 6 2004, In : Journal of Biological Chemistry. 279, 6, p. 4241-4249 9 p.

Research output: Contribution to journalArticle

115 Scopus citations

Extensive phosphorylation of Smoothened in Hedgehog pathway activation

Zhang, C., Williams, E. H., Guo, Y., Lum, L. & Beachy, P. A., Dec 28 2004, In : Proceedings of the National Academy of Sciences of the United States of America. 101, 52, p. 17900-17907 8 p.

Research output: Contribution to journalArticle

126 Scopus citations

The hedgehog response network: Sensors, switches, and routers

Lum, L. & Beachy, P. A., Jun 18 2004, In : Science. 304, 5678, p. 1755-1759 5 p.

Research output: Contribution to journalReview article

629 Scopus citations