Cell Biology - Division U

Research Output 1996 2019

2019

CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation

Tuladhar, R., Yeu, Y., Tyler Piazza, J., Tan, Z., Rene Clemenceau, J., Wu, X., Barrett, Q., Herbert, J., Mathews, D. H., Kim, J., Hyun Hwang, T. & Lum, L., Dec 1 2019, In : Nature communications. 10, 1, 4056.

Research output: Contribution to journalArticle

Open Access
Clustered Regularly Interspaced Short Palindromic Repeats
mutagenesis
deletion
Mutagenesis
insertion

Stereoselective fatty acylation is essential for the release of lipidated WNT proteins from the acyltransferase Porcupine (PORCN)

Tuladhar, R., Yarravarapu, N., Ma, Y., Zhang, C., Herbert, J., Kim, J., Chen, C. & Lum, L., Jan 1 2019, In : Journal of Biological Chemistry. 294, 16, p. 6273-6282 10 p.

Research output: Contribution to journalArticle

Porcupines
Acylation
Animals
Acyltransferases
Proteins
2018

A synthetic combinatorial approach to disabling deviant Hedgehog signaling

Fan, C. W., Yarravarapu, N., Shi, H., Kulak, O., Kim, J., Chen, C. & Lum, L., Dec 1 2018, In : Scientific Reports. 8, 1, 1133.

Research output: Contribution to journalArticle

Histone Deacetylases
Medulloblastoma
Critical Pathways
Basal Cell Carcinoma
DNA-Binding Proteins
4 Citations (Scopus)

Chemical Modulation of WNT Signaling in Cancer

Zhang, L. S. & Lum, L., Jan 1 2018, WNT Signaling in Health and Disease. Elsevier B.V., p. 245-269 25 p. (Progress in Molecular Biology and Translational Science; vol. 153).

Research output: Chapter in Book/Report/Conference proceedingChapter

Porcupines
Acyltransferases
Antineoplastic Agents
Regeneration
Melanoma
1 Citation (Scopus)

EGF receptor and mTORC1 are novel therapeutic targets in nonseminomatous germ cell tumors

Chen, K., Fustino, N. J., Shukla, A. A., Stroup, E. K., Budhipramono, A., Ateek, C., Stuart, S. H., Yamaguchi, K., Kapur, P., Frazier, A. L., Lum, L., Looijenga, L. H. J., Laetsch, T. W., Rakheja, D. & Amatruda, J. F., May 1 2018, In : Molecular Cancer Therapeutics. 17, 5, p. 1079-1089 11 p.

Research output: Contribution to journalArticle

Epidermal Growth Factor Receptor
Germ Cell and Embryonal Neoplasms
Seminoma
Therapeutics
Embryonal Carcinoma
1 Citation (Scopus)

Installation of a cancer promoting WNT/SIX1 signaling axis by the oncofusion protein MLL-AF9

Zhang, L. S., Kang, X., Lu, J., Zhang, Y., Wu, X., Wu, G., Zheng, J., Tuladhar, R., Shi, H., Wang, Q., Morlock, L., Yao, H., Huang, J-S., Maire, P., Kim, J., Williams, N. S., Xu, J., Chen, C., Zhang, C. & Lum, L., Jan 1 2018, (Accepted/In press) In : EBioMedicine.

Research output: Contribution to journalArticle

Catenins
Acute Myeloid Leukemia
Neoplasms
Proteins
Genetic Translocation
3 Citations (Scopus)

Regulation of tankyrase activity by a catalytic domain dimer interface

Fan, C., Yarravarapu, N., Chen, H., Kulak, O., Dasari, P., Herbert, J., Yamaguchi, K., Lum, L. & Zhang, X., Jan 1 2018, (Accepted/In press) In : Biochemical and Biophysical Research Communications.

Research output: Contribution to journalArticle

Tankyrases
Dimers
Catalytic Domain
Adenosine Diphosphate
Oligomerization
2017
17 Citations (Scopus)

Blockade to pathological remodeling of infarcted heart tissue using a porcupine antagonist

Moon, J., Zhou, H., Zhang, L. S., Tan, W., Liu, Y., Zhang, S., Morlock, L. K., Bao, X., Palecek, S. P., Feng, J. Q., Williams, N. S., Amatruda, J. F., Olson, E. N., Bassel-Duby, R. & Lum, L., Feb 14 2017, In : Proceedings of the National Academy of Sciences of the United States of America. 114, 7, p. 1649-1654 6 p.

Research output: Contribution to journalArticle

Porcupines
Non-Fibrillar Collagens
Regeneration
Wnt Proteins
Acyltransferases
11 Citations (Scopus)

Extra-mitochondrial prosurvival BCL-2 proteins regulate gene transcription by inhibiting the SUFU tumour suppressor

Wu, X., Zhang, L. S., Toombs, J., Kuo, Y. C., Piazza, J. T., Tuladhar, R., Barrett, Q., Fan, C. W., Zhang, X., Walensky, L. D., Kool, M., Cheng, S. Y., Brekken, R., Opferman, J. T., Green, D. R., Moldoveanu, T. & Lum, L., Sep 29 2017, In : Nature Cell Biology. 19, 10, p. 1226-1236 11 p.

Research output: Contribution to journalArticle

Neoplasms
Proteins
Mitochondrial Membranes
DNA-Binding Proteins
Growth
2016
7 Citations (Scopus)

Development of a triazole class of highly potent Porcn inhibitors

You, L., Zhang, C., Yarravarapu, N., Morlock, L., Wang, X., Zhang, L., Williams, N. S., Lum, L. & Chen, C., Dec 15 2016, In : Bioorganic and Medicinal Chemistry Letters. 26, 24, p. 5891-5895 5 p.

Research output: Contribution to journalArticle

Porcupines
Triazoles
Scaffolds
Wnt Proteins
Acyltransferases

Monitoring wnt protein acylation using an in vitro cyclo- addition reaction

Tuladhar, R., Yarravarapu, N. & Lum, L., Jan 1 2016, Methods in Molecular Biology. Humana Press Inc., p. 11-16 6 p. (Methods in Molecular Biology; vol. 1481).

Research output: Chapter in Book/Report/Conference proceedingChapter

Wnt Proteins
Acylation
Cycloaddition Reaction
Click Chemistry
Immobilized Proteins
2015
13 Citations (Scopus)
Regenerative Medicine
Decision Making
Decision making
Molecules
Wnt Proteins
31 Citations (Scopus)

Disruption of Wnt/β-catenin signaling and telomeric shortening are inextricable consequences of tankyrase inhibition in human cells

Kulak, O., Chen, H., Holohan, B., Wu, X., He, H., Borek, D., Otwinowski, Z., Yamaguchi, K., Garofalo, L. A., Ma, Z., Wright, W., Chen, C., Shay, J. W., Zhang, X. & Lum, L., 2015, In : Molecular and Cellular Biology. 35, 14, p. 2425-2435 11 p.

Research output: Contribution to journalArticle

Tankyrases
Catenins
Telomere Homeostasis
Telomere
Porcupines

Erratum: The hedgehog pathway effector smoothened exhibits signaling competency in the absence of ciliary accumulation (Chemistry and Biology (2014) 21 (1680-689))

Fan, C. W., Chen, B., Franco, I., Lu, J., Shi, H., Wei, S., Wang, C., Wu, X., Tang, W., Roth, M. G., Williams, N. S., Hirsch, E., Chen, C. & Lum, L., Apr 23 2015, In : Chemistry and Biology. 22, 4, p. 559 1 p.

Research output: Contribution to journalArticle

10 Citations (Scopus)

Fatty acyl donor selectivity in membrane bound o-acyltransferases and communal cell fate decision-making

Tuladhar, R. & Lum, L., Apr 1 2015, In : Biochemical Society Transactions. 43, p. 235-239 5 p.

Research output: Contribution to journalArticle

Acyltransferases
Decision Making
Decision making
Membranes
Transferases
6 Citations (Scopus)

Role of the exocyst complex component Sec6/8 in genomic stability

Torres, M. J., Pandita, R. K., Kulak, O., Kumar, R., Formstecher, E., Horikoshi, N., Mujoo, K., Hunt, C. R., Zhao, Y., Lum, L., Zaman, A., Yeaman, C., White, M. A. & Pandita, T. K., 2015, In : Molecular and Cellular Biology. 35, 21, p. 3633-3645 13 p.

Research output: Contribution to journalArticle

Genomic Instability
DNA Damage
Chromatin
Chromatids
Ionizing Radiation
2014
15 Citations (Scopus)

Computational detection and suppression of sequence-specific off-target phenotypes from whole genome RNAi screens

Zhong, R., Kim, J., Kim, H. S., Kim, M., Lum, L., Levine, B., Xiao, G., White, M. A. & Xie, Y., 2014, In : Nucleic Acids Research. 42, 13, p. 8214-8222 9 p.

Research output: Contribution to journalArticle

RNA Interference
Small Interfering RNA
Genome
Phenotype
MicroRNAs
33 Citations (Scopus)

KIF14 promotes AKT phosphorylation and contributes to chemoresistance in triple-negative breast cancer

Singel, S. M., Cornelius, C., Zaganjor, E., Batten, K., Sarode, V. R., Buckley, D. L., Peng, Y., John, G. B., Li, H. C., Sadeghi, N., Wright, W. E., Lum, L., Corson, T. W. & Shay, J. W., 2014, In : Neoplasia (United States). 16, 3, p. 247-256 10 p., e2.

Research output: Contribution to journalArticle

Triple Negative Breast Neoplasms
Kinesin
Phosphorylation
docetaxel
Breast
12 Citations (Scopus)

The hedgehog pathway effector smoothened exhibits signaling competency in the absence of ciliary accumulation

Fan, C. W., Chen, B., Franco, I., Lu, J., Shi, H., Wei, S., Wang, C., Wu, X., Tang, W., Roth, M. G., Williams, N. S., Hirsch, E., Chen, C. & Lum, L., Dec 18 2014, In : Chemistry and Biology. 21, 12, p. 1680-1689 10 p.

Research output: Contribution to journalArticle

Cilia
Phosphatidylinositol-4-Phosphate 3-Kinase
Hedgehog Proteins
Chemical activation
Cell membranes
2013
4 Citations (Scopus)
Signal transduction
RNA Interference
Luciferases
Cultured Cells
Signal Transduction
33 Citations (Scopus)

A targeted RNAi screen of the breast cancer genome identifies KIF14 and TLN1 as genes that modulate docetaxel chemosensitivity in triple-negative breast cancer

Singel, S. M., Cornelius, C., Batten, K., Fasciani, G., Wright, W. E., Lum, L. & Shay, J. W., Apr 15 2013, In : Clinical Cancer Research. 19, 8, p. 2061-2070 10 p.

Research output: Contribution to journalArticle

docetaxel
Talin
Triple Negative Breast Neoplasms
Kinesin
RNA Interference
1 Citation (Scopus)

Erratum: Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation (Nature Cell Biology (2013) 15 (1035-1044))

Das, A., Tanigawa, S., Karner, C. M., Xin, M., Lum, L., Chen, C., Olson, E. N., Perantoni, A. O. & Carroll, T. J., Oct 2013, In : Nature Cell Biology. 15, 10, p. 1260 1 p.

Research output: Contribution to journalArticle

Signaling: Making a leaner Hedgehog

Fan, C. W., Tuladhar, R. & Lum, L., Apr 2013, In : Nature Chemical Biology. 9, 4, p. 217-218 2 p.

Research output: Contribution to journalArticle

125 Citations (Scopus)

Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation

Das, A., Tanigawa, S., Karner, C. M., Xin, M., Lum, L., Chen, C., Olson, E. N., Perantoni, A. O. & Carroll, T. J., Sep 2013, In : Nature Cell Biology. 15, 9, p. 1035-1044 10 p.

Research output: Contribution to journalArticle

Cell Differentiation
Stem Cells
Kidney
Nephrons
Cadherins
40 Citations (Scopus)

The development of highly potent inhibitors for porcupine

Wang, X., Moon, J., Dodge, M. E., Pan, X., Zhang, L., Hanson, J. M., Tuladhar, R., Ma, Z., Shi, H., Williams, N. S., Amatruda, J. F., Carroll, T. J., Lum, L. & Chen, C., Mar 28 2013, In : Journal of Medicinal Chemistry. 56, 6, p. 2700-2704 5 p.

Research output: Contribution to journalArticle

Porcupines
Wnt Proteins
Lipoylation
Kidney Tubules
Zebrafish
2012
53 Citations (Scopus)

Diverse chemical scaffolds support direct inhibition of the membrane-bound O-acyltransferase porcupine

Dodge, M. E., Moon, J., Tuladhar, R., Lu, J., Jacob, L. S., Zhang, L. S., Shi, H., Wang, X., Moro, E., Mongera, A., Argenton, F., Karner, C. M., Carroll, T. J., Chen, C., Amatruda, J. F. & Lum, L., Jun 29 2012, In : Journal of Biological Chemistry. 287, 27, p. 23246-23254 9 p.

Research output: Contribution to journalArticle

Porcupines
Wnt Proteins
Acyltransferases
Catenins
Scaffolds
105 Citations (Scopus)

In vivo Wnt signaling tracing through a transgenic biosensor fish reveals novel activity domains

Moro, E., Ozhan-Kizil, G., Mongera, A., Beis, D., Wierzbicki, C., Young, R. M., Bournele, D., Domenichini, A., Valdivia, L. E., Lum, L., Chen, C., Amatruda, J. F., Tiso, N., Weidinger, G. & Argenton, F., Jun 15 2012, In : Developmental Biology. 366, 2, p. 327-340 14 p.

Research output: Contribution to journalArticle

Catenins
Biosensing Techniques
Fishes
Developmental Biology
Wnt Signaling Pathway
44 Citations (Scopus)

Mechanistic insight into Myc stabilization in breast cancer involving aberrant Axin1 expression

Zhang, X., Farrell, A. S., Daniel, C. J., Arnold, H., Scanlan, C., Laraway, B. J., Janghorban, M., Lum, L., Chen, D., Troxell, M. & Sears, R., Feb 21 2012, In : Proceedings of the National Academy of Sciences of the United States of America. 109, 8, p. 2790-2795 6 p.

Research output: Contribution to journalArticle

Phosphorylation
Threonine
Breast Neoplasms
Serine
Protein Stability
5 Citations (Scopus)

The Chemistry and Biology of Nakiterpiosin - C-nor-D-Homosteroids

Gao, S., Wang, Q., Wang, G., Lomenick, B., Liu, J., Fan, C. W., Deng, L. W., Huang, J., Lum, L. & Chen, C., 2012, (Accepted/In press) In : Synlett. 23

Research output: Contribution to journalArticle

Homosteroids
Biomimetics
Biological Products
nakiterpiosin
51 Citations (Scopus)

The unusual case of porcupine

Lum, L. & Clevers, H., Aug 24 2012, In : Science. 337, 6097, p. 922-923 2 p.

Research output: Contribution to journalArticle

Porcupines
Wnt Signaling Pathway
Neoplasms
Therapeutics
2011
156 Citations (Scopus)

Canonical Wnt9b signaling balances progenitor cell expansion and differentiation during kidney development

Karner, C. M., Das, A., Ma, Z., Self, M., Chen, C., Lum, L., Oliver, G. & Carroll, T. J., Apr 2011, In : Development. 138, 7, p. 1247-1257 11 p.

Research output: Contribution to journalArticle

Nephrons
Catenins
Cell Differentiation
Stem Cells
Kidney
40 Citations (Scopus)
Signal transduction
Neoplastic Stem Cells
Stem cells
Signal Transduction
Tissue
68 Citations (Scopus)

Genome-wide RNAi screen reveals disease-associated genes that are common to Hedgehog and Wnt signaling

Jacob, L. S., Wu, X., Dodge, M. E., Fan, C. W., Kulak, O., Chen, B., Tang, W., Wang, B., Amatruda, J. F. & Lum, L., Jan 25 2011, In : Science Signaling. 4, 157, ra4.

Research output: Contribution to journalArticle

Hedgehogs
RNA Interference
Genes
Genome
Wnt Signaling Pathway
94 Citations (Scopus)

Small molecule Wnt inhibitors enhance the efficiency of BMP-4-directed cardiac differentiation of human pluripotent stem cells

Ren, Y., Lee, M. Y., Schliffke, S., Paavola, J., Amos, P. J., Ge, X., Ye, M., Zhu, S., Senyei, G., Lum, L., Ehrlich, B. E. & Qyang, Y., Sep 2011, In : Journal of Molecular and Cellular Cardiology. 51, 3, p. 280-287 8 p.

Research output: Contribution to journalArticle

Bone Morphogenetic Protein 4
Induced Pluripotent Stem Cells
Pluripotent Stem Cells
Cardiac Myocytes
Action Potentials
2010
47 Citations (Scopus)

Chemical and biological studies of nakiterpiosin and nakiterpiosinone

Gao, S., Wang, Q., Huang, L. J. S., Lum, L. & Chen, C., Jan 13 2010, In : Journal of the American Chemical Society. 132, 1, p. 371-383 13 p.

Research output: Contribution to journalArticle

Antimitotic Agents
Signal transduction
Hedgehogs
Antineoplastic Agents
Homosteroids
32 Citations (Scopus)

Tankyrase is necessary for canonical Wnt signaling during kidney development

Karner, C. M., Merkel, C. E., Dodge, M., Ma, Z., Lu, J., Chen, C., Lum, L. & Carroll, T. J., 2010, In : Developmental Dynamics. 239, 7, p. 2014-2023 10 p.

Research output: Contribution to journalArticle

Tankyrases
Kidney
Wnt Signaling Pathway
Catenins
Zebrafish
2009
887 Citations (Scopus)

Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer

Chen, B., Dodge, M. E., Tang, W., Lu, J., Ma, Z., Fan, C. W., Wei, S., Hao, W., Kilgore, J., Williams, N. S., Roth, M. G., Amatruda, J. F., Chen, C. & Lum, L., Feb 6 2009, In : Nature Chemical Biology. 5, 2, p. 100-107 8 p.

Research output: Contribution to journalArticle

Wnt Signaling Pathway
Regeneration
Wnt Proteins
Catenins
Axin Protein
86 Citations (Scopus)

Structure-activity relationship studies of small-molecule inhibitors of Wnt response

Lu, J., Ma, Z., Hsieh, J. C., Fan, C. W., Chen, B., Longgood, J. C., Williams, N. S., Amatruda, J. F., Lum, L. & Chen, C., Jul 15 2009, In : Bioorganic and Medicinal Chemistry Letters. 19, 14, p. 3825-3827 3 p.

Research output: Contribution to journalArticle

Structure-Activity Relationship
Molecules
Neoplasms
Therapeutics
2008
121 Citations (Scopus)

A genome-wide RNAi screen for Wnt/β-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer

Tang, W., Dodge, M., Gundapaneni, D., Michnoff, C., Roth, M. & Lum, L., Jul 15 2008, In : Proceedings of the National Academy of Sciences of the United States of America. 105, 28, p. 9697-9702 6 p.

Research output: Contribution to journalArticle

TCF Transcription Factors
Catenins
Wnt Signaling Pathway
RNA Interference
Colorectal Neoplasms
2007
50 Citations (Scopus)

Deconstructing the hedgehog pathway in development and disease

Jacob, L. & Lum, L., Oct 5 2007, In : Science. 318, 5847, p. 66-68 3 p.

Research output: Contribution to journalArticle

Hedgehogs
Hedgehog Proteins
Drosophila
Embryonic Development
Homeostasis
10 Citations (Scopus)

Hedgehog signaling pathway.

Jacob, L. & Lum, L., Oct 9 2007, In : Science's STKE : signal transduction knowledge environment. 2007, 407

Research output: Contribution to journalArticle

Tissue homeostasis
Signal transduction
Signal Transduction
Homeostasis
Neoplasms
6 Citations (Scopus)

Hedgehog signaling pathway in Drosophila.

Jacob, L. & Lum, L., Oct 9 2007, In : Science's STKE : signal transduction knowledge environment. 2007, 407

Research output: Contribution to journalArticle

Signal transduction
Hedgehogs
Drosophila
Molecules
Human Development
2006
43 Citations (Scopus)

A genomewide screen for components of the RNAi pathway in Drosophila cultured cells

Dorner, S., Lum, L., Kim, M., Paro, R., Beachy, P. A. & Green, R., Aug 8 2006, In : Proceedings of the National Academy of Sciences of the United States of America. 103, 32, p. 11880-11885 6 p.

Research output: Contribution to journalArticle

RNA Interference
Drosophila
Cultured Cells
RNA Stability
Drosophila melanogaster
331 Citations (Scopus)

Minimizing the risk of reporting false positives in large-scale RNAi screens

Echeverri, C. J., Beachy, P. A., Baum, B., Boutros, M., Buchholz, F., Chanda, S. K., Downward, J., Ellenberg, J., Fraser, A. G., Hacohen, N., Hahn, W. C., Jackson, A. L., Kiger, A., Linsley, P. S., Lum, L., Ma, Y., Mathey-Prévôt, B., Root, D. E., Sabatini, D. M., Taipale, J. & 2 others, Perrimon, N. & Bernards, R., Oct 2006, In : Nature Methods. 3, 10, p. 777-779 3 p.

Research output: Contribution to journalArticle

216 Citations (Scopus)

Prevalence of off-target effects in Drosophila RNA interference screens

Ma, Y., Creanga, A., Lum, L. & Beachy, P. A., Sep 21 2006, In : Nature. 443, 7109, p. 359-363 5 p.

Research output: Contribution to journalArticle

RNA Interference
Drosophila
Double-Stranded RNA
Genome
RNA
146 Citations (Scopus)

The Ihog Cell-Surface Proteins Bind Hedgehog and Mediate Pathway Activation

Yao, S., Lum, L. & Beachy, P., Apr 21 2006, In : Cell. 125, 2, p. 343-357 15 p.

Research output: Contribution to journalArticle

Hedgehog Proteins
Hedgehogs
Fibronectins
Membrane Proteins
Chemical activation
2004
113 Citations (Scopus)

Evidence for a Critical Role of the Tumor Necrosis Factor α Convertase (TACE) in Ectodomain Shedding of the p75 Neurotrophin Receptor (p75NTR)

Weskamp, G., Schlöndorff, J., Lum, L., Becherer, J. D., Kim, T. W., Saftig, P., Hartmann, D., Murphy, G. & Blobel, C. P., Feb 6 2004, In : Journal of Biological Chemistry. 279, 6, p. 4241-4249 9 p.

Research output: Contribution to journalArticle

Nerve Growth Factor Receptor
Tumor Necrosis Factor-alpha
Cricetulus
Ovary
Proteins
123 Citations (Scopus)

Extensive phosphorylation of Smoothened in Hedgehog pathway activation

Zhang, C., Williams, E. H., Guo, Y., Lum, L. & Beachy, P. A., Dec 28 2004, In : Proceedings of the National Academy of Sciences of the United States of America. 101, 52, p. 17900-17907 8 p.

Research output: Contribution to journalArticle

Hedgehogs
Phosphorylation
Casein Kinase I
Cyclic AMP-Dependent Protein Kinases
Tail
619 Citations (Scopus)

The hedgehog response network: Sensors, switches, and routers

Lum, L. & Beachy, P. A., Jun 18 2004, In : Science. 304, 5678, p. 1755-1759 5 p.

Research output: Contribution to journalArticle

Body Patterning
Kinesin
Zinc Fingers
Growth
Cell Differentiation
2003
180 Citations (Scopus)

Hedgehog signal transduction via Smoothened association with a cytoplasmic complex scaffolded by the atypical kinesin, Costal-2

Lum, L., Zhang, C., Oh, S., Mann, R. K., Von Kessler, D. P., Taipale, J., Weis-Garcia, F., Gong, R., Wang, B. & Beachy, P. A., Nov 2003, In : Molecular Cell. 12, 5, p. 1261-1274 14 p.

Research output: Contribution to journalArticle

Kinesin
Signal Transduction
Phosphorylation
Transcription Factors
Membranes