Cell Biology - Nicastro Lab

Research Output 1997 2019

2019

FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia

Lin, J., Le, T. V., Augspurger, K., Tritschler, D., Bower, R., Fu, G., Perrone, C., O'Toole, E. T., VanderWaal Mills, K., Dymek, E., Smith, E., Nicastro, D. & Porter, M. E., Oct 1 2019, In : Molecular biology of the cell. 30, 21, p. 2659-2680 22 p.

Research output: Contribution to journalArticle

Open Access
Dyneins
Cilia
Electron Microscope Tomography
Insertional Mutagenesis
Protein Subunits

Heterotrophic carbon metabolism and energy acquisition in Candidatus Thioglobus singularis strain PS1, a member of the SUP05 clade of marine Gammaproteobacteria

Spietz, R. L., Lundeen, R. A., Zhao, X., Nicastro, D., Ingalls, A. E. & Morris, R. M., Jan 1 2019, In : Environmental Microbiology.

Research output: Contribution to journalArticle

Gammaproteobacteria
Heterotrophic Processes
Calvin cycle
gamma-Proteobacteria
Carbon Cycle
5 Citations (Scopus)

Ionotropic Receptors Specify the Morphogenesis of Phasic Sensors Controlling Rapid Thermal Preference in Drosophila

Budelli, G., Ni, L., Berciu, C., van Giesen, L., Knecht, Z. A., Chang, E. C., Kaminski, B., Silbering, A. F., Samuel, A., Klein, M., Benton, R., Nicastro, D. & Garrity, P. A., Feb 20 2019, In : Neuron. 101, 4, p. 738-747.e3

Research output: Contribution to journalArticle

Morphogenesis
Drosophila
Hot Temperature
Thermosensing
Temperature
10 Citations (Scopus)

Mdm1 maintains endoplasmic reticulum homeostasis by spatially regulating lipid droplet biogenesis

Hariri, H., Speer, N., Bowerman, J., Rogers, S., Fu, G., Reetz, E., Datta, S., Feathers, J. R., Ugrankar, R., Nicastro, D. & Henne, W. M., Apr 1 2019, In : The Journal of cell biology. 218, 4, p. 1319-1334 16 p.

Research output: Contribution to journalArticle

Open Access
Endoplasmic Reticulum
Homeostasis
Fatty Acids
Vacuoles
Yeasts
4 Citations (Scopus)

Membrane bridging by Munc13-1 is crucial for neurotransmitter release

Quade, B., Camacho, M., Zhao, X., Orlando, M., Trimbuch, T., Xu, J., Li, W., Nicastro, D., Rosenmund, C. & Rizo-Rey, J., Feb 28 2019, In : eLife. 8

Research output: Contribution to journalArticle

Open Access
Synaptic Vesicles
Neurotransmitter Agents
Synaptic Membranes
Cell membranes
Membranes

Morphological Plasticity in a Sulfur-Oxidizing Marine Bacterium from the SUP05 Clade Enhances Dark Carbon Fixation

Shah, V., Zhao, X., Lundeen, R. A., Ingalls, A. E., Nicastro, D. & Morris, R. M., May 7 2019, In : mBio. 10, 3

Research output: Contribution to journalArticle

Open Access
Carbon Cycle
Sulfur
Bacteria
Oxygen
Cell Size
2 Citations (Scopus)

PACRG and FAP20 form the inner junction of axonemal doublet microtubules and regulate ciliary motility

Dymek, E. E., Lin, J., Fu, G., Porter, M. E., Nicastro, D. & Smith, E. F., Jul 15 2019, In : Molecular biology of the cell. 30, 15, p. 1805-1816 12 p.

Research output: Contribution to journalArticle

Open Access
Microtubules
Dyneins
Electron Microscope Tomography
Beak
Axoneme
2018
23 Citations (Scopus)

Asymmetric distribution and spatial switching of dynein activity generates ciliary motility

Lin, J. & Nicastro, D., Apr 27 2018, In : Science. 360, 6387, eaar1968.

Research output: Contribution to journalArticle

Dyneins
Flagella
Sea Urchins
Cilia
Organelles
1 Citation (Scopus)

Cellular Uptake of A Taurine-Modified, Ester Bond-Decorated D-Peptide Derivative via Dynamin-Based Endocytosis and Macropinocytosis

Zhou, J., Du, X., Berciu, C., Del Signore, S. J., Chen, X., Yamagata, N., Rodal, A. A., Nicastro, D. & Xu, B., Feb 7 2018, In : Molecular Therapy. 26, 2, p. 648-658 11 p.

Research output: Contribution to journalArticle

Dynamins
Taurine
Endocytosis
Esters
Peptides
6 Citations (Scopus)

Ciliary proteins Fap43 and Fap44 interact with each other and are essential for proper cilia and flagella beating

Urbanska, P., Joachimiak, E., Bazan, R., Fu, G., Poprzeczko, M., Fabczak, H., Nicastro, D. & Wloga, D., Apr 23 2018, (Accepted/In press) In : Cellular and Molecular Life Sciences. p. 1-15 15 p.

Research output: Contribution to journalArticle

Dyneins
Flagella
Cilia
Tetrahymena thermophila
Axoneme
4 Citations (Scopus)

DRC2/CCDC65 is a central hub for assembly of the nexin–dynein regulatory complex and other regulators of ciliary and flagellar motility

Bower, R., Tritschler, D., VanderWaal Mills, K., Heuser, T., Nicastro, D. & Portera, M. E., Jan 15 2018, In : Molecular Biology of the Cell. 29, 2, p. 137-153 17 p.

Research output: Contribution to journalArticle

Kartagener Syndrome
Chlamydomonas
Dyneins
Mutation
Calmodulin
9 Citations (Scopus)

Tetrahymena RIB72A and RIB72B are microtubule inner proteins in the ciliary doublet microtubules

Stoddard, D., Zhao, Y., Bayless, B. A., Gui, L., Louka, P., Dave, D., Suryawanshi, S., Tomasi, R. F. X., Dupuis-Williams, P., Baroud, C. N., Gaertig, J., Winey, M. & Nicastro, D., Oct 15 2018, In : Molecular Biology of the Cell. 29, 21, p. 2566-2577 12 p.

Research output: Contribution to journalArticle

Microtubule Proteins
Tetrahymena
Microtubules
Cilia
Basal Bodies
7 Citations (Scopus)

The I1 dynein-associated tether and tether head complex is a conserved regulator of ciliary motility

Fu, G., Wang, Q., Phan, N., Urbanska, P., Joachimiak, E., Lin, J., Wloga, D. & Nicastro, D., May 1 2018, In : Molecular Biology of the Cell. 29, 9, p. 1048-1059 12 p.

Research output: Contribution to journalArticle

Dyneins
Head
Axonemal Dyneins
Critical Pathways
Cilia
5 Citations (Scopus)

The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length

Hunter, E. L., Lechtreck, K., Fu, G., Hwang, J., Lin, H., Gokhale, A., Alford, L. M., Lewis, B., Yamamoto, R., Kamiya, R., Yang, F., Nicastro, D., Dutcher, S. K., Wirschell, M., Sale, W. S. & Holzbaur, E., Apr 15 2018, In : Molecular Biology of the Cell. 29, 8, p. 886-896 11 p.

Research output: Contribution to journalArticle

Dyneins
Cilia
Axonemal Dyneins
Cytoplasm
Axoneme
2017
14 Citations (Scopus)

Centriolar remodeling underlies basal body maturation during ciliogenesis in Caenorhabditis elegans

Nechipurenko, I. V., Berciu, C., Sengupta, P. & Nicastro, D., Apr 15 2017, In : eLife. 6, e25686.

Research output: Contribution to journalArticle

Basal Bodies
Caenorhabditis elegans
Neurons
Centrioles
Cilia
13 Citations (Scopus)

Three-dimensional structure of the ultraoligotrophic marine bacterium "Candidatus pelagibacter ubique"

Zhao, X., Schwartz, C. L., Pierson, J., Giovannoni, S. J., McIntosh, J. R. & Nicastro, D., Jan 1 2017, In : Applied and Environmental Microbiology. 83, 3, e02807-16.

Research output: Contribution to journalArticle

membrane
Bacteria
bacterium
bacteria
open ocean
2016
51 Citations (Scopus)

Enzyme-Instructed Self-Assembly for Spatiotemporal Profiling of the Activities of Alkaline Phosphatases on Live Cells

Zhou, J., Du, X., Berciu, C., He, H., Shi, J., Nicastro, D. & Xu, B., Aug 11 2016, In : Chem. 1, 2, p. 246-263 18 p.

Research output: Contribution to journalArticle

Phosphatases
phosphatase
Self assembly
Alkaline Phosphatase
cancer
8 Citations (Scopus)

The nexin link and B-tubule glutamylation maintain the alignment of outer doublets in the ciliary axoneme

Alford, L. M., Stoddard, D., Li, J. H., Hunter, E. L., Tritschler, D., Bower, R., Nicastro, D., Porter, M. E. & Sale, W. S., Jun 1 2016, In : Cytoskeleton. 73, 7, p. 331-340 10 p.

Research output: Contribution to journalArticle

Axoneme
Adenosine Triphosphate
Tubulin
Microtubules
2015
3 Citations (Scopus)

Assembly of actin filaments and microtubules in Nwk F-BAR-induced membrane deformations

Kelley, C. F., Becalska, A. N., Berciu, C., Nicastro, D. & Rodal, A. A., Jan 1 2015, In : Communicative and Integrative Biology. 8, 2, p. 1-5 5 p.

Research output: Contribution to journalArticle

microfilaments
Actin Cytoskeleton
Microtubules
microtubules
pseudopodia
15 Citations (Scopus)

ATP Consumption of Eukaryotic Flagella Measured at a Single-Cell Level

Chen, D. T. N., Heymann, M., Fraden, S., Nicastro, D. & Dogic, Z., Dec 15 2015, In : Biophysical Journal. 109, 12, p. 2562-2573 12 p.

Research output: Contribution to journalArticle

Flagella
Adenosine Triphosphate
Axoneme
Dyneins
Sea Urchins
51 Citations (Scopus)

Cryo-electron tomography reveals ciliary defects underlying human RSPH1 primary ciliary dyskinesia

Lin, J., Yin, W., Smith, M. C., Song, K., Leigh, M. W., Zariwala, M. A., Knowles, M. R., Ostrowski, L. E. & Nicastro, D., Jan 1 2015, In : Nature Communications. 5, 6727.

Research output: Contribution to journalArticle

Electron Microscope Tomography
Kartagener Syndrome
Cilia
Tomography
tomography
15 Citations (Scopus)

DRC3 connects the N-DRC to dynein g to regulate flagellar waveform

Awata, J., Song, K., Lin, J., King, S. M., Sanderson, M. J., Nicastro, D. & Witman, G. B., Aug 1 2015, In : Molecular Biology of the Cell. 26, 15, p. 2788-2800 13 p.

Research output: Contribution to journalArticle

Dyneins
Electron Microscope Tomography
Axoneme
Chlamydomonas
Paralysis
8 Citations (Scopus)

FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c

Vasudevan, K. K., Song, K., Alford, L. M., Sale, W. S., Dymek, E. E., Smith, E. F., Hennessey, T., Joachimiak, E., Urbanska, P., Wloga, D., Dentler, W., Nicastro, D. & Gaertig, J., Jan 1 2015, In : Molecular Biology of the Cell. 26, 4, p. 696-710 15 p.

Research output: Contribution to journalArticle

Dyneins
Microtubules
Electron Microscope Tomography
Macromolecular Substances
Tetrahymena
18 Citations (Scopus)

In situ localization of N and C termini of subunits of the flagellar nexin-dynein regulatory complex (N-DRC) using SNAP tag and cryo-electron tomography

Song, K., Awata, J., Tritschler, D., Bower, R., Witman, G. B., Porter, M. E. & Nicastro, D., Feb 27 2015, In : Journal of Biological Chemistry. 290, 9, p. 5341-5353 13 p.

Research output: Contribution to journalArticle

Electron Microscope Tomography
Dyneins
Tomography
Flagella
Electrons
16 Citations (Scopus)

Membrane Charge Directs the Outcome of F-BAR Domain Lipid Binding and Autoregulation

Kelley, C. F., Messelaar, E. M., Eskin, T. L., Wang, S., Song, K., Vishnia, K., Becalska, A. N., Shupliakov, O., Hagan, M. F., Danino, D., Sokolova, O. S., Nicastro, D. & Rodal, A. A., Dec 22 2015, In : Cell Reports. 13, 11, p. 2597-2609 13 p.

Research output: Contribution to journalArticle

Homeostasis
Membranes
Lipids
src Homology Domains
Chemical analysis
19 Citations (Scopus)

The CSC proteins FAP61 and FAP251 build the basal substructures of radial spoke 3 in cilia

Urbanska, P., Song, K., Joachimiak, E., Krzemien-Ojak, L., Koprowski, P., Hennessey, T., Jerka-Dziadosz, M., Fabczak, H., Gaertig, J., Nicastro, D. & Wloga, D., Jan 1 2015, In : Molecular Biology of the Cell. 26, 8, p. 1463-1475 13 p.

Research output: Contribution to journalArticle

Cilia
Calmodulin
Dyneins
Chlamydomonas
Tetrahymena
2014
78 Citations (Scopus)

A high-resolution morphological and ultrastructural map of anterior sensory cilia and glia in Caenorhabditis elegans

Doroquez, D. B., Berciu, C., Anderson, J. R., Sengupta, P. & Nicastro, D., Mar 25 2014, In : eLife. 2014, 3, e01948.

Research output: Contribution to journalArticle

Cilia
Caenorhabditis elegans
Neuroglia
Electron microscopy
Tomography
25 Citations (Scopus)

Critical roles for multiple formins during cardiac myofibril development and repair

Rosado, M., Barber, C. F., Berciu, C., Feldman, S., Birren, S. J., Nicastro, D. & Goode, B. L., Mar 15 2014, In : Molecular Biology of the Cell. 25, 6, p. 811-827 17 p.

Research output: Contribution to journalArticle

Myofibrils
Sarcomeres
RNA Interference
Actomyosin
Muscle Development
43 Citations (Scopus)

Insights into the structure and function of ciliary and flagellar doublet microtubules: Tektins, Ca2+-binding proteins, and stable protofilaments

Linck, R., Fu, X., Lin, J., Ouch, C., Schefter, A., Steffen, W., Warren, P. & Nicastro, D., Jun 20 2014, In : Journal of Biological Chemistry. 289, 25, p. 17427-17444 18 p.

Research output: Contribution to journalArticle

Microtubules
Carrier Proteins
Tubulin
Proteins
Dyneins
55 Citations (Scopus)

Membrane deformation and scission by the HSV-1 nuclear egress complex

Bigalke, J. M., Heuser, T., Nicastro, D. & Heldwein, E. E., Jun 11 2014, In : Nature Communications. 5, 4131.

Research output: Contribution to journalArticle

egress
Human Herpesvirus 1
cleavage
Nuclear Envelope
membranes
52 Citations (Scopus)

Robust excitons inhabit soft supramolecular nanotubes

Eisele, D. M., Arias, D. H., Fu, X., Bloemsma, E. A., Steiner, C. P., Jensen, R. A., Rebentrost, P., Eisele, H., Tokmakoff, A., Lloyd, S., Nelson, K. A., Nicastro, D., Knoester, J. & Bawendi, M. G., Aug 19 2014, In : Proceedings of the National Academy of Sciences of the United States of America. 111, 33, p. E3367-E3375

Research output: Contribution to journalArticle

Nanotubes
Chlorobi
Coloring Agents
Light
Equipment and Supplies
20 Citations (Scopus)

Structural Correlates of Rotavirus Cell Entry

Abdelhakim, A. H., Salgado, E. N., Fu, X., Pasham, M., Nicastro, D., Kirchhausen, T. & Harrison, S. C., Jan 1 2014, In : PLoS Pathogens. 10, 9

Research output: Contribution to journalArticle

Rotavirus
Cytosol
Membranes
Virion
Viral Structures
61 Citations (Scopus)

Structural mechanism of the dynein power stroke

Lin, J., Okada, K., Raytchev, M., Smith, M. C. & Nicastro, D., Jan 1 2014, In : Nature Cell Biology. 16, 5, p. 479-485 7 p.

Research output: Contribution to journalArticle

Dyneins
Stroke
Microtubules
Electron Microscope Tomography
Sperm Tail

Supramolecular self-assembly inside living mammalian cells

Gao, Y., Nieuwendaal, R., Hammouda, B., Berciu, C., Nicastro, D., Douglas, J., Xu, B. & Horkay, F., Jan 1 2014, In : Materials Research Society Symposium Proceedings. 1622

Research output: Contribution to journalArticle

Self assembly
Nanostructures
Cells
Transmission electron microscopy
2013
27 Citations (Scopus)

Conserved structural motifs in the central pair complex of eukaryotic flagella

Carbajal-González, B. I., Heuser, T., Fu, X., Lin, J., Smith, B. W., Mitchell, D. R. & Nicastro, D., Feb 1 2013, In : Cytoskeleton. 70, 2, p. 101-120 20 p.

Research output: Contribution to journalArticle

Strongylocentrotus
Chlamydomonas
Flagella
Cilia
Sea Urchins
22 Citations (Scopus)

Formation of membrane ridges and scallops by the F-BAR protein nervous wreck

Becalska, A. N., Kelley, C. F., Berciu, C., Stanishneva-Konovalova, T. B., Fu, X., Wang, S., Sokolova, O. S., Nicastro, D. & Rodal, A. A., Aug 1 2013, In : Molecular Biology of the Cell. 24, 15, p. 2406-2418 13 p.

Research output: Contribution to journalArticle

Pectinidae
Membranes
Proteins
amphiphysin
Eukaryotic Cells
44 Citations (Scopus)

Probing nanoscale self-assembly of nonfluorescent small molecules inside live mammalian cells

Gao, Y., Berciu, C., Kuang, Y., Shi, J., Nicastro, D. & Xu, B., Oct 22 2013, In : ACS Nano. 7, 10, p. 9055-9063 9 p.

Research output: Contribution to journalArticle

Self assembly
self assembly
Cells
Molecules
assemblies
38 Citations (Scopus)

The MIA complex is a conserved and novel dynein regulator essential for normal ciliary motility

Yamamoto, R., Song, K., Yanagisawa, H. A., Fox, L., Yagi, T., Wirschell, M., Hirono, M., Kamiya, R., Nicastro, D. & Sale, W. S., Apr 15 2013, In : Journal of Cell Biology. 201, 2, p. 263-278 16 p.

Research output: Contribution to journalArticle

Dyneins
Axoneme
Axonemal Dyneins
Electron Microscope Tomography
Chlamydomonas reinhardtii
2012
53 Citations (Scopus)

Cryoelectron tomography reveals doublet-specific structures and unique interactions in the I1 dynein

Heuser, T., Barber, C. F., Lin, J., Krell, J., Rebesco, M., Porter, M. E. & Nicastro, D., Jul 24 2012, In : Proceedings of the National Academy of Sciences of the United States of America. 109, 30

Research output: Contribution to journalArticle

Dyneins
Tomography
Axoneme
Chlamydomonas
Flagella

Cryo-Electron Tomography Reveals Novel Features of Cilia and Flagella

Nicastro, D., Heuser, T., Lin, J. & Barber, C. F., Jan 1 2012, In : Microscopy and Microanalysis. 18, p. 554-555 2 p.

Research output: Contribution to journalArticle

Tomography
tomography
Electrons
electrons
27 Citations (Scopus)

One of the Nine Doublet Microtubules of Eukaryotic Flagella Exhibits Unique and Partially Conserved Structures

Lin, J., Heuser, T., Song, K., Fu, X. & Nicastro, D., Oct 10 2012, In : PLoS One. 7, 10, e46494.

Research output: Contribution to journalArticle

Dyneins
Flagella
Strongylocentrotus
flagellum
Microtubules
130 Citations (Scopus)

Reconfigurable self-assembly through chiral control of interfacial tension

Gibaud, T., Barry, E., Zakhary, M. J., Henglin, M., Ward, A., Yang, Y., Berciu, C., Oldenbourg, R., Hagan, M. F., Nicastro, D., Meyer, R. B. & Dogic, Z., Jan 19 2012, In : Nature. 481, 7381, p. 348-351 4 p.

Research output: Contribution to journalArticle

Surface Tension
Liquid Crystals
Frustration
Surface-Active Agents
Membranes
42 Citations (Scopus)

The CSC connects three major axonemal complexes involved in dynein regulation

Heuser, T., Dymek, E. E., Lin, J., Smith, E. F. & Nicastro, D., Aug 15 2012, In : Molecular Biology of the Cell. 23, 16, p. 3143-3155 13 p.

Research output: Contribution to journalArticle

Dyneins
Calmodulin
Flagella
Electron Microscope Tomography
Chlamydomonas
31 Citations (Scopus)

The structural heterogeneity of radial spokes in cilia and flagella is conserved

Lin, J., Heuser, T., Carbajal-González, B. I., Song, K. & Nicastro, D., Feb 1 2012, In : Cytoskeleton. 69, 2, p. 88-100 13 p.

Research output: Contribution to journalArticle

Dyneins
Flagella
Cilia
Strongylocentrotus purpuratus
Sperm Tail

Three-Dimensional Reconstruction of the Amphid Sensory Primary Cilia of C. elegans by Serial Section Electron Microscopy

Berciu, C., Doroquez, D. B., Sengupta, P. & Nicastro, D., Jan 1 2012, In : Microscopy and Microanalysis. 18, p. 538-539 2 p.

Research output: Contribution to journalArticle

Electron microscopy
electron microscopy
49 Citations (Scopus)

Three-dimensional structure of the radial spokes reveals heterogeneity and interactions with dyneins in Chlamydomonas flagella

Barber, C. F., Heuser, T., Carbajal-González, B. I., Botchkarev, V. V. & Nicastro, D., Jan 1 2012, In : Molecular Biology of the Cell. 23, 1, p. 111-120 10 p.

Research output: Contribution to journalArticle

Chlamydomonas
Dyneins
Flagella
Axonemal Dyneins
Electron Microscope Tomography
2011
50 Citations (Scopus)

Building blocks of the nexin-dynein regulatory complex in chlamydomonas flagella

Lin, J., Tritschler, D., Song, K., Barber, C. F., Cobb, J. S., Porter, M. E. & Nicastro, D., Aug 19 2011, In : Journal of Biological Chemistry. 286, 33, p. 29175-29191 17 p.

Research output: Contribution to journalArticle

Chlamydomonas
Dyneins
Flagella
Phosphorylation
Protein Isoforms
117 Citations (Scopus)

Cilia-like beating of active microtubule bundles

Sanchez, T., Welch, D., Nicastro, D. & Dogic, Z., Jul 22 2011, In : Science. 333, 6041, p. 456-459 4 p.

Research output: Contribution to journalArticle

Flagella
Cilia
Microtubules
66 Citations (Scopus)

Cryo-electron tomography reveals conserved features of doublet microtubules in flagella

Nicastro, D., Fu, X., Heuser, T., Tso, A., Porter, M. E. & Linck, R. W., Oct 18 2011, In : Proceedings of the National Academy of Sciences of the United States of America. 108, 42

Research output: Contribution to journalArticle

Electron Microscope Tomography
Flagella
Tubulin
Microtubules
Axoneme
75 Citations (Scopus)

Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome

Gopalakrishnan, J., Mennella, V., Blachon, S., Zhai, B., Smith, A. H., Megraw, T. L., Nicastro, D., Gygi, S. P., Agard, D. A. & Avidor-Reiss, T., Jun 29 2011, In : Nature Communications. 2, 1, 359.

Research output: Contribution to journalArticle

Centrosome
Scaffolds
tethering
proteins
cell division