Cell Biology - Nicastro Lab

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2020

Absolute proteomic quantification reveals design principles of sperm flagellar chemosensation

Trötschel, C., Hamzeh, H., Alvarez, L., Pascal, R., Lavryk, F., Bönigk, W., Körschen, H. G., Müller, A., Poetsch, A., Rennhack, A., Gui, L., Nicastro, D., Strünker, T., Seifert, R. & Kaupp, U. B., Feb 17 2020, In : EMBO Journal. 39, 4, e102723.

Research output: Contribution to journalArticle

Open Access
1 Scopus citations

Chlamydomonas PKD2 organizes mastigonemes, hair-like glycoprotein polymers on cilia

Liu, P., Lou, X., Wingfield, J. L., Lin, J., Nicastro, D. & Lechtreck, K., Jun 1 2020, In : Journal of Cell Biology. 219, 6, 202001122.

Research output: Contribution to journalArticle

Open Access

In situ structure determination at nanometer resolution using TYGRESS

Song, K., Shang, Z., Fu, X., Lou, X., Grigorieff, N. & Nicastro, D., Feb 1 2020, In : Nature methods. 17, 2, p. 201-208 8 p.

Research output: Contribution to journalArticle

5 Scopus citations
2019

FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia

Lin, J., Le, T. V., Augspurger, K., Tritschler, D., Bower, R., Fu, G., Perrone, C., O'Toole, E. T., VanderWaal Mills, K., Dymek, E., Smith, E., Nicastro, D. & Porter, M. E., Oct 1 2019, In : Molecular biology of the cell. 30, 21, p. 2659-2680 22 p.

Research output: Contribution to journalArticle

Open Access
3 Scopus citations

Heterotrophic carbon metabolism and energy acquisition in Candidatus Thioglobus singularis strain PS1, a member of the SUP05 clade of marine Gammaproteobacteria

Spietz, R. L., Lundeen, R. A., Zhao, X., Nicastro, D., Ingalls, A. E. & Morris, R. M., Jul 2019, In : Environmental Microbiology. 21, 7, p. 2391-2401 11 p.

Research output: Contribution to journalArticle

Ionotropic Receptors Specify the Morphogenesis of Phasic Sensors Controlling Rapid Thermal Preference in Drosophila

Budelli, G., Ni, L., Berciu, C., van Giesen, L., Knecht, Z. A., Chang, E. C., Kaminski, B., Silbering, A. F., Samuel, A., Klein, M., Benton, R., Nicastro, D. & Garrity, P. A., Feb 20 2019, In : Neuron. 101, 4, p. 738-747.e3

Research output: Contribution to journalArticle

12 Scopus citations

Mdm1 maintains endoplasmic reticulum homeostasis by spatially regulating lipid droplet biogenesis

Hariri, H., Speer, N., Bowerman, J., Rogers, S., Fu, G., Reetz, E., Datta, S., Feathers, J. R., Ugrankar, R., Nicastro, D. & Henne, W. M., Jan 1 2019, In : Journal of Cell Biology. 218, 4, p. 1319-1334 16 p.

Research output: Contribution to journalArticle

Open Access
21 Scopus citations

Membrane bridging by Munc13-1 is crucial for neurotransmitter release

Quade, B., Camacho, M., Zhao, X., Orlando, M., Trimbuch, T., Xu, J., Li, W., Nicastro, D., Rosenmund, C. & Rizo-Rey, J., Feb 28 2019, In : eLife. 8

Research output: Contribution to journalArticle

Open Access
8 Scopus citations

Morphological plasticity in a sulfur-oxidizing marine bacterium from the SUP05 clade enhances dark carbon fixation

Shah, V., Zhao, X., Lundeen, R. A., Ingalls, A. E., Nicastro, D. & Morris, R. M., May 1 2019, In : mBio. 10, 3, e00216-19.

Research output: Contribution to journalArticle

Open Access
2 Scopus citations

PACRG and FAP20 form the inner junction of axonemal doublet microtubules and regulate ciliary motility

Dymek, E. E., Lin, J., Fu, G., Porter, M. E., Nicastro, D. & Smith, E. F., Jul 15 2019, In : Molecular biology of the cell. 30, 15, p. 1805-1816 12 p.

Research output: Contribution to journalArticle

Open Access
5 Scopus citations

Scaffold subunits support associated subunit assembly in the Chlamydomonas ciliary nexin-dynein regulatory complex

Gui, L., Song, K., Tritschler, D., Bower, R., Yan, S., Dai, A., Augspurger, K., Sakizadeh, J., Grzemska, M., Ni, T., Porter, M. E. & Nicastro, D., Nov 12 2019, In : Proceedings of the National Academy of Sciences of the United States of America. 116, 46, p. 23152-23162 11 p.

Research output: Contribution to journalArticle

Open Access
1 Scopus citations

Structural organization of the C1a-e-c supercomplex within the ciliary central apparatus

Fu, G., Zhao, L., Dymek, E., Hou, Y., Song, K., Phan, N., Shang, Z., Smith, E. F., Witman, G. B. & Nicastro, D., Dec 2 2019, In : Journal of Cell Biology. 218, 12, p. 4236-4251 16 p.

Research output: Contribution to journalArticle

Open Access
2 Scopus citations
2018

Asymmetric distribution and spatial switching of dynein activity generates ciliary motility

Lin, J. & Nicastro, D., Apr 27 2018, In : Science. 360, 6387, eaar1968.

Research output: Contribution to journalArticle

49 Scopus citations

Cellular Uptake of A Taurine-Modified, Ester Bond-Decorated D-Peptide Derivative via Dynamin-Based Endocytosis and Macropinocytosis

Zhou, J., Du, X., Berciu, C., Del Signore, S. J., Chen, X., Yamagata, N., Rodal, A. A., Nicastro, D. & Xu, B., Feb 7 2018, In : Molecular Therapy. 26, 2, p. 648-658 11 p.

Research output: Contribution to journalArticle

4 Scopus citations

Ciliary proteins Fap43 and Fap44 interact with each other and are essential for proper cilia and flagella beating

Urbanska, P., Joachimiak, E., Bazan, R., Fu, G., Poprzeczko, M., Fabczak, H., Nicastro, D. & Wloga, D., Dec 1 2018, In : Cellular and Molecular Life Sciences. 75, 24, p. 4479-4493 15 p.

Research output: Contribution to journalArticle

14 Scopus citations

DRC2/CCDC65 is a central hub for assembly of the nexin–dynein regulatory complex and other regulators of ciliary and flagellar motility

Bower, R., Tritschler, D., VanderWaal Mills, K., Heuser, T., Nicastro, D. & Portera, M. E., Jan 15 2018, In : Molecular Biology of the Cell. 29, 2, p. 137-153 17 p.

Research output: Contribution to journalArticle

10 Scopus citations

Tetrahymena RIB72A and RIB72B are microtubule inner proteins in the ciliary doublet microtubules

Stoddard, D., Zhao, Y., Bayless, B. A., Gui, L., Louka, P., Dave, D., Suryawanshi, S., Tomasi, R. F. X., Dupuis-Williams, P., Baroud, C. N., Gaertig, J., Winey, M. & Nicastro, D., Oct 15 2018, In : Molecular Biology of the Cell. 29, 21, p. 2566-2577 12 p.

Research output: Contribution to journalArticle

11 Scopus citations

The I1 dynein-associated tether and tether head complex is a conserved regulator of ciliary motility

Fu, G., Wang, Q., Phan, N., Urbanska, P., Joachimiak, E., Lin, J., Wloga, D. & Nicastro, D., May 1 2018, In : Molecular Biology of the Cell. 29, 9, p. 1048-1059 12 p.

Research output: Contribution to journalArticle

15 Scopus citations

The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length

Hunter, E. L., Lechtreck, K., Fu, G., Hwang, J., Lin, H., Gokhale, A., Alford, L. M., Lewis, B., Yamamoto, R., Kamiya, R., Yang, F., Nicastro, D., Dutcher, S. K., Wirschell, M., Sale, W. S. & Holzbaur, E., Apr 15 2018, In : Molecular Biology of the Cell. 29, 8, p. 886-896 11 p.

Research output: Contribution to journalArticle

9 Scopus citations
2017

Centriolar remodeling underlies basal body maturation during ciliogenesis in Caenorhabditis elegans

Nechipurenko, I. V., Berciu, C., Sengupta, P. & Nicastro, D., Apr 15 2017, In : eLife. 6, e25686.

Research output: Contribution to journalArticle

18 Scopus citations

Three-dimensional structure of the ultraoligotrophic marine bacterium "Candidatus pelagibacter ubique"

Zhao, X., Schwartz, C. L., Pierson, J., Giovannoni, S. J., McIntosh, J. R. & Nicastro, D., Jan 1 2017, In : Applied and Environmental Microbiology. 83, 3, e02807-16.

Research output: Contribution to journalArticle

17 Scopus citations
2016

Enzyme-Instructed Self-Assembly for Spatiotemporal Profiling of the Activities of Alkaline Phosphatases on Live Cells

Zhou, J., Du, X., Berciu, C., He, H., Shi, J., Nicastro, D. & Xu, B., Aug 11 2016, In : Chem. 1, 2, p. 246-263 18 p.

Research output: Contribution to journalArticle

67 Scopus citations

The nexin link and B-tubule glutamylation maintain the alignment of outer doublets in the ciliary axoneme

Alford, L. M., Stoddard, D., Li, J. H., Hunter, E. L., Tritschler, D., Bower, R., Nicastro, D., Porter, M. E. & Sale, W. S., Jun 1 2016, In : Cytoskeleton. 73, 7, p. 331-340 10 p.

Research output: Contribution to journalArticle

10 Scopus citations
2015

Assembly of actin filaments and microtubules in Nwk F-BAR-induced membrane deformations

Kelley, C. F., Becalska, A. N., Berciu, C., Nicastro, D. & Rodal, A. A., Jan 1 2015, In : Communicative and Integrative Biology. 8, 2, p. 1-5 5 p.

Research output: Contribution to journalArticle

4 Scopus citations

ATP Consumption of Eukaryotic Flagella Measured at a Single-Cell Level

Chen, D. T. N., Heymann, M., Fraden, S., Nicastro, D. & Dogic, Z., Dec 15 2015, In : Biophysical Journal. 109, 12, p. 2562-2573 12 p.

Research output: Contribution to journalArticle

18 Scopus citations

DRC3 connects the N-DRC to dynein g to regulate flagellar waveform

Awata, J., Song, K., Lin, J., King, S. M., Sanderson, M. J., Nicastro, D. & Witman, G. B., Aug 1 2015, In : Molecular biology of the cell. 26, 15, p. 2788-2800 13 p.

Research output: Contribution to journalArticle

20 Scopus citations

FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c

Vasudevan, K. K., Song, K., Alford, L. M., Sale, W. S., Dymek, E. E., Smith, E. F., Hennessey, T., Joachimiak, E., Urbanska, P., Wloga, D., Dentler, W., Nicastro, D. & Gaertig, J., Feb 15 2015, In : Molecular biology of the cell. 26, 4, p. 696-710 15 p.

Research output: Contribution to journalArticle

8 Scopus citations

In situ localization of N and C termini of subunits of the flagellar nexin-dynein regulatory complex (N-DRC) using SNAP tag and cryo-electron tomography

Song, K., Awata, J., Tritschler, D., Bower, R., Witman, G. B., Porter, M. E. & Nicastro, D., Feb 27 2015, In : Journal of Biological Chemistry. 290, 9, p. 5341-5353 13 p.

Research output: Contribution to journalArticle

22 Scopus citations

Membrane Charge Directs the Outcome of F-BAR Domain Lipid Binding and Autoregulation

Kelley, C. F., Messelaar, E. M., Eskin, T. L., Wang, S., Song, K., Vishnia, K., Becalska, A. N., Shupliakov, O., Hagan, M. F., Danino, D., Sokolova, O. S., Nicastro, D. & Rodal, A. A., Dec 22 2015, In : Cell Reports. 13, 11, p. 2597-2609 13 p.

Research output: Contribution to journalArticle

21 Scopus citations

The CSC proteins FAP61 and FAP251 build the basal substructures of radial spoke 3 in cilia

Urbanska, P., Song, K., Joachimiak, E., Krzemien-Ojak, L., Koprowski, P., Hennessey, T., Jerka-Dziadosz, M., Fabczak, H., Gaertig, J., Nicastro, D. & Wloga, D., Apr 15 2015, In : Molecular biology of the cell. 26, 8, p. 1463-1475 13 p.

Research output: Contribution to journalArticle

25 Scopus citations
2014

A high-resolution morphological and ultrastructural map of anterior sensory cilia and glia in Caenorhabditis elegans

Doroquez, D. B., Berciu, C., Anderson, J. R., Sengupta, P. & Nicastro, D., Mar 25 2014, In : eLife. 2014, 3, e01948.

Research output: Contribution to journalArticle

88 Scopus citations

Critical roles for multiple formins during cardiac myofibril development and repair

Rosado, M., Barber, C. F., Berciu, C., Feldman, S., Birren, S. J., Nicastro, D. & Goode, B. L., Mar 15 2014, In : Molecular biology of the cell. 25, 6, p. 811-827 17 p.

Research output: Contribution to journalArticle

28 Scopus citations

Cryo-electron tomography reveals ciliary defects underlying human RSPH1 primary ciliary dyskinesia

Lin, J., Yin, W., Smith, M. C., Song, K., Leigh, M. W., Zariwala, M. A., Knowles, M. R., Ostrowski, L. E. & Nicastro, D., 2014, In : Nature communications. 5, 5727.

Research output: Contribution to journalArticle

63 Scopus citations

Insights into the structure and function of ciliary and flagellar doublet microtubules: Tektins, Ca2+-binding proteins, and stable protofilaments

Linck, R., Fu, X., Lin, J., Ouch, C., Schefter, A., Steffen, W., Warren, P. & Nicastro, D., Jun 20 2014, In : Journal of Biological Chemistry. 289, 25, p. 17427-17444 18 p.

Research output: Contribution to journalArticle

44 Scopus citations

Membrane deformation and scission by the HSV-1 nuclear egress complex

Bigalke, J. M., Heuser, T., Nicastro, D. & Heldwein, E. E., Jun 11 2014, In : Nature communications. 5, 4131.

Research output: Contribution to journalArticle

64 Scopus citations

Robust excitons inhabit soft supramolecular nanotubes

Eisele, D. M., Arias, D. H., Fu, X., Bloemsma, E. A., Steiner, C. P., Jensen, R. A., Rebentrost, P., Eisele, H., Tokmakoff, A., Lloyd, S., Nelson, K. A., Nicastro, D., Knoester, J. & Bawendi, M. G., Aug 19 2014, In : Proceedings of the National Academy of Sciences of the United States of America. 111, 33, p. E3367-E3375

Research output: Contribution to journalArticle

59 Scopus citations

Structural Correlates of Rotavirus Cell Entry

Abdelhakim, A. H., Salgado, E. N., Fu, X., Pasham, M., Nicastro, D., Kirchhausen, T. & Harrison, S. C., Sep 1 2014, In : PLoS pathogens. 10, 9

Research output: Contribution to journalArticle

22 Scopus citations

Structural mechanism of the dynein power stroke

Lin, J., Okada, K., Raytchev, M., Smith, M. C. & Nicastro, D., Jan 1 2014, In : Nature Cell Biology. 16, 5, p. 479-485 7 p.

Research output: Contribution to journalArticle

67 Scopus citations
2013

Conserved structural motifs in the central pair complex of eukaryotic flagella

Carbajal-González, B. I., Heuser, T., Fu, X., Lin, J., Smith, B. W., Mitchell, D. R. & Nicastro, D., Feb 1 2013, In : Cytoskeleton. 70, 2, p. 101-120 20 p.

Research output: Contribution to journalArticle

34 Scopus citations

Formation of membrane ridges and scallops by the F-BAR protein nervous wreck

Becalska, A. N., Kelley, C. F., Berciu, C., Stanishneva-Konovalova, T. B., Fu, X., Wang, S., Sokolova, O. S., Nicastro, D. & Rodal, A. A., Aug 1 2013, In : Molecular biology of the cell. 24, 15, p. 2406-2418 13 p.

Research output: Contribution to journalArticle

23 Scopus citations

Probing nanoscale self-assembly of nonfluorescent small molecules inside live mammalian cells

Gao, Y., Berciu, C., Kuang, Y., Shi, J., Nicastro, D. & Xu, B., Oct 22 2013, In : ACS Nano. 7, 10, p. 9055-9063 9 p.

Research output: Contribution to journalArticle

48 Scopus citations

The MIA complex is a conserved and novel dynein regulator essential for normal ciliary motility

Yamamoto, R., Song, K., Yanagisawa, H. A., Fox, L., Yagi, T., Wirschell, M., Hirono, M., Kamiya, R., Nicastro, D. & Sale, W. S., Apr 15 2013, In : Journal of Cell Biology. 201, 2, p. 263-278 16 p.

Research output: Contribution to journalArticle

42 Scopus citations
2012

Cryoelectron tomography reveals doublet-specific structures and unique interactions in the I1 dynein

Heuser, T., Barber, C. F., Lin, J., Krell, J., Rebesco, M., Porter, M. E. & Nicastro, D., Jul 24 2012, In : Proceedings of the National Academy of Sciences of the United States of America. 109, 30, p. E2067-E2076

Research output: Contribution to journalArticle

58 Scopus citations

Cryo-Electron Tomography Reveals Novel Features of Cilia and Flagella

Nicastro, D., Heuser, T., Lin, J. & Barber, C. F., Jan 1 2012, In : Microscopy and Microanalysis. 18, p. 554-555 2 p.

Research output: Contribution to journalArticle

One of the Nine Doublet Microtubules of Eukaryotic Flagella Exhibits Unique and Partially Conserved Structures

Lin, J., Heuser, T., Song, K., Fu, X. & Nicastro, D., Oct 10 2012, In : PloS one. 7, 10, e46494.

Research output: Contribution to journalArticle

32 Scopus citations

Reconfigurable self-assembly through chiral control of interfacial tension

Gibaud, T., Barry, E., Zakhary, M. J., Henglin, M., Ward, A., Yang, Y., Berciu, C., Oldenbourg, R., Hagan, M. F., Nicastro, D., Meyer, R. B. & Dogic, Z., Jan 19 2012, In : Nature. 481, 7381, p. 348-351 4 p.

Research output: Contribution to journalArticle

141 Scopus citations

The CSC connects three major axonemal complexes involved in dynein regulation

Heuser, T., Dymek, E. E., Lin, J., Smith, E. F. & Nicastro, D., Aug 15 2012, In : Molecular biology of the cell. 23, 16, p. 3143-3155 13 p.

Research output: Contribution to journalArticle

49 Scopus citations

The structural heterogeneity of radial spokes in cilia and flagella is conserved

Lin, J., Heuser, T., Carbajal-González, B. I., Song, K. & Nicastro, D., Feb 1 2012, In : Cytoskeleton. 69, 2, p. 88-100 13 p.

Research output: Contribution to journalArticle

34 Scopus citations

Three-Dimensional Reconstruction of the Amphid Sensory Primary Cilia of C. elegans by Serial Section Electron Microscopy

Berciu, C., Doroquez, D. B., Sengupta, P. & Nicastro, D., Jan 1 2012, In : Microscopy and Microanalysis. 18, p. 538-539 2 p.

Research output: Contribution to journalArticle

Three-dimensional structure of the radial spokes reveals heterogeneity and interactions with dyneins in Chlamydomonas flagella

Barber, C. F., Heuser, T., Carbajal-González, B. I., Botchkarev, V. V. & Nicastro, D., Jan 1 2012, In : Molecular biology of the cell. 23, 1, p. 111-120 10 p.

Research output: Contribution to journalArticle

51 Scopus citations