Physiology - Division D

Research Output 1982 2019

2019
Permeability
Anti-Bacterial Agents
Membranes
Microbial Viability
Mycobacterium smegmatis
1 Citation (Scopus)
Transient Receptor Potential Channels
Cations
Amphibian Proteins
Mutation
TRPV Cation Channels
1 Citation (Scopus)

Interaction of the Mechanosensitive Channel, MscS, with the Membrane Bilayer through Lipid Intercalation into Grooves and Pockets

Rasmussen, T., Rasmussen, A., Yang, L., Kaul, C., Black, S., Galbiati, H., Conway, S. J., Miller, S., Blount, P. & Booth, I. R., Jan 1 2019, In : Journal of Molecular Biology.

Research output: Contribution to journalArticle

Open Access
Lipid Bilayers
Lipids
Membranes
Cardiolipins
Membrane Lipids
2018
2 Citations (Scopus)

Effects of Low Intensity Focused Ultrasound on Liposomes Containing Channel proteins

Babakhanian, M., Yang, L., Nowroozi, B., Saddik, G., Boodaghians, L., Blount, P. & Grundfest, W., Nov 22 2018, In : Scientific Reports. 8, 1, 1 p.

Research output: Contribution to journalArticle

Liposomes
Membranes
Proteins
Elasticity
Membrane Lipids
2 Citations (Scopus)

Engineering a pH-Sensitive Liposomal MRI Agent by Modification of a Bacterial Channel

Yang, L. M., Zheng, H., Ratnakar, J. S., Adebesin, B. Y., Do, Q. N., Kovacs, Z. & Blount, P., Jan 1 2018, (Accepted/In press) In : Small.

Research output: Contribution to journalArticle

Magnetic resonance
Magnetic Resonance Imaging
Imaging techniques
Nanopores
Gadolinium
1 Citation (Scopus)

The phosphorylation site T613 in the β-subunit of rat epithelial Na+ channel (ENaC) modulates channel inhibition by Nedd4-2

Krueger, B., Yang, L., Korbmacher, C. & Rauh, R., Apr 1 2018, In : Pflugers Archiv European Journal of Physiology. 470, 4, p. 649-660 12 p.

Research output: Contribution to journalArticle

Epithelial Sodium Channels
Phosphorylation
Rats
Oocytes
Extracellular Signal-Regulated MAP Kinases
2016
6 Citations (Scopus)

Dihydrostreptomycin Directly Binds to, Modulates, and Passes through the MscL Channel Pore

Wray, R., Iscla, I., Gao, Y., Li, H., Wang, J. & Blount, P., Jun 9 2016, In : PLoS Biology. 14, 6, e1002473.

Research output: Contribution to journalArticle

Dihydrostreptomycin Sulfate
dihydrostreptomycin
osmolarity
Genetic Testing
Protein Biosynthesis
2015
17 Citations (Scopus)

A new antibiotic with potent activity targets MscL

Iscla, I., Wray, R., Blount, P., Larkins-Ford, J., Conery, A. L., Ausubel, F. M., Ramu, S., Kavanagh, A., Huang, J. X., Blaskovich, M. A., Cooper, M. A., Obregon-Henao, A., Orme, I., Tjandra, E. S., Stroeher, U. H., Brown, M. H., Macardle, C., Van Holst, N., Ling Tong, C., Slattery, A. D. & 3 others, Gibson, C. T., Raston, C. L. & Boulos, R. A., Jul 29 2015, In : Journal of Antibiotics. 68, 7, p. 453-462 10 p.

Research output: Contribution to journalArticle

Anti-Bacterial Agents
Methicillin-Resistant Staphylococcus aureus
Computer Simulation
Bacteria
Caenorhabditis elegans
6 Citations (Scopus)

Mutations in a conserved domain of E. coli MscS to the most conserved superfamily residue leads to kinetic changes

Malcolm, H. R. & Blount, P., Sep 4 2015, In : PLoS One. 10, 9, e0136756.

Research output: Contribution to journalArticle

Escherichia coli
mutation
kinetics
Mutation
Kinetics
8 Citations (Scopus)

Scanning MscL channels with targeted post-translational modifications for functional alterations

Iscla, I., Wray, R., Eaton, C. & Blount, P., Sep 14 2015, In : PLoS One. 10, 9, e0137994.

Research output: Contribution to journalArticle

post-translational modification
Post Translational Protein Processing
Scanning
Escherichia coli
Cysteine
14 Citations (Scopus)

The mechanosensitive channel of small conductance (MscS) functions as a Jack-in-the box

Malcolm, H. R., Blount, P. & Maurer, J. A., Jan 1 2015, In : Biochimica et Biophysica Acta - General Subjects. 1848, 1 Pt A, p. 159-166 8 p.

Research output: Contribution to journalArticle

Jacks
Lipids
Lipid bilayers
Lipid Bilayers
Hydrophobic and Hydrophilic Interactions
2014
3 Citations (Scopus)

Dynamics of protein-protein interactions at the mscl periplasmic-lipid interface

Zhong, D., Yang, L. M. & Blount, P., Jan 21 2014, In : Biophysical Journal. 106, 2, p. 375-381 7 p.

Research output: Contribution to journalArticle

Disulfides
Cysteine
Periplasmic Proteins
Lipids
Mutation
4 Citations (Scopus)

Electrostatics at the membrane define MscL channel mechanosensitivity and kinetics

Zhong, D. & Blount, P., Dec 1 2014, In : FASEB Journal. 28, 12, p. 5234-5241 8 p.

Research output: Contribution to journalArticle

Static Electricity
Electrostatics
Membranes
Kinetics
Proteins
21 Citations (Scopus)

Streptomycin potency is dependent on MscL channel expression

Iscla, I., Wray, R., Wei, S., Posner, B. & Blount, P., 2014, In : Nature Communications. 5, 4891.

Research output: Contribution to journalArticle

streptomycin
Streptomycin
Dihydrostreptomycin Sulfate
efflux
antibiotics
2013
7 Citations (Scopus)

Chimeras Reveal a Single Lipid-Interface Residue that Controls MscL Channel Kinetics as well as Mechanosensitivity

Yang, L. M., Zhong, D. & Blount, P., 2013, In : Cell Reports. 3, 2, p. 520-527 8 p.

Research output: Contribution to journalArticle

Escherichia coli Proteins
Energy barriers
Escherichia coli
Hysteresis
Staphylococcus aureus
19 Citations (Scopus)

Improving the design of a MscL-based triggered nanovalve

Iscla, I., Eaton, C., Parker, J., Wray, R., Kovács, Z. & Blount, P., Mar 2013, In : Biosensors. 3, 1, p. 171-184 14 p.

Research output: Contribution to journalArticle

Bacterial Proteins
Clamping devices
Constriction
Liposomes
Assays
18 Citations (Scopus)

Phosphatidylinositol is crucial for the mechanosensitivity of mycobacterium tuberculosis MscL

Zhong, D. & Blount, P., Aug 13 2013, In : Biochemistry. 52, 32, p. 5415-5420 6 p.

Research output: Contribution to journalArticle

Phosphatidylinositols
Mycobacterium tuberculosis
Escherichia coli
Membranes
Lipids
2012
37 Citations (Scopus)

Sensing and responding to membrane tension: The bacterial MscL channel as a model system

Iscla, I. & Blount, P., Jul 18 2012, In : Biophysical Journal. 103, 2, p. 169-174 6 p.

Research output: Contribution to journalArticle

Osmoregulation
Proprioception
Kidney
Membranes
Touch
35 Citations (Scopus)

Structure and molecular mechanism of an anion-selective mechanosensitive channel of small conductance

Zhang, X., Wang, J., Feng, Y., Ge, J., Li, W., Sun, W., Iscla, I., Yu, J., Blount, P., Li, Y. & Yang, M., Oct 30 2012, In : Proceedings of the National Academy of Sciences of the United States of America. 109, 44, p. 18180-18185 6 p.

Research output: Contribution to journalArticle

Molecular Structure
Anions
Ions
Thermoanaerobacter
Static Electricity
4 Citations (Scopus)
Protein Interaction Domains and Motifs
Protein Interaction Maps
Iris
Disulfides
Osmolar Concentration
86 Citations (Scopus)

The MscS and MscL families of mechanosensitive channels act as microbial emergency release valves

Bootha, I. R. & Blount, P., Sep 2012, In : Journal of Bacteriology. 194, 18, p. 4802-4809 8 p.

Research output: Contribution to journalArticle

Emergencies
Pressure
Mycoplasma
Environmental Exposure
Atmosphere
24 Citations (Scopus)

Three routes to modulate the pore size of the Mscl channel/nanovalve

Yang, L. M., Wray, R., Parker, J., Wilson, D., Duran, R. S. & Blount, P., Feb 28 2012, In : ACS Nano. 6, 2, p. 1134-1141 8 p.

Research output: Contribution to journalArticle

bundles
Pore size
routes
porosity
helices
2011
15 Citations (Scopus)

An in vivo screen reveals protein-lipid interactions crucial for gating a mechanosensitive channel

Iscla, I., Wray, R. & Blount, P., Feb 2011, In : FASEB Journal. 25, 2, p. 694-702 9 p.

Research output: Contribution to journalArticle

Lipids
Membranes
Mutagenesis
Proteins
Site-Directed Mutagenesis
12 Citations (Scopus)

Manipulating the permeation of charged compounds through the MscL nanovalve

Yang, L. M. & Blount, P., Jan 2011, In : FASEB Journal. 25, 1, p. 428-434 7 p.

Research output: Contribution to journalArticle

Permeation
Nanopores
Membranes
Equipment and Supplies
Ion Channels
28 Citations (Scopus)

The oligomeric state of the truncated mechanosensitive channel of large conductance shows no variance in vivo

Iscla, I., Wray, R. & Blount, P., Sep 2011, In : Protein Science. 20, 9, p. 1638-1642 5 p.

Research output: Contribution to journalArticle

Detergents
Proteins
Crystal structure
Membranes
Protein S
2010
47 Citations (Scopus)
stoichiometry
membrane proteins
Stoichiometry
detergents
Detergents
2009
24 Citations (Scopus)

An open-pore structure of the mechanosensitive channel MscL derived by determining transmembrane domain interactions upon gating

Li, Y., Wray, R., Eaton, C. & Blount, P., Jul 2009, In : FASEB Journal. 23, 7, p. 2197-2204 8 p.

Research output: Contribution to journalArticle

Pore structure
Static Electricity
Electrostatics
Aptitude
Structural Models
42 Citations (Scopus)

Cholesterol depletion of the plasma membrane prevents activation of the epithelial sodium channel (ENaC) by SGK1

Krueger, B., Haerteis, S., Yang, L., Hartner, A., Rauh, R., Korbmacher, C. & Diakov, A., Nov 30 2009, In : Cellular Physiology and Biochemistry. 24, 5-6, p. 605-618 14 p.

Research output: Contribution to journalArticle

Epithelial Sodium Channels
Cyclodextrins
Cholesterol
Cell Membrane
Lipids
2008
44 Citations (Scopus)

On the structure of the N-terminal domain of the MscL channel: Helical bundle or membrane interface

Iscla, I., Wray, R. & Blount, P., Sep 1 2008, In : Biophysical Journal. 95, 5, p. 2283-2291 9 p.

Research output: Contribution to journalArticle

Mycobacterium tuberculosis
Disulfides
Membranes
Structural Models
Cysteine
31 Citations (Scopus)

Voltage-induced gating of the mechanosensitive MscL ion channel reconstituted in a tethered lipid bilayer membrane

Andersson, M., Okeyo, G., Wilson, D., Keizer, H., Moe, P., Blount, P., Fine, D., Dodabalapur, A. & Duran, R. S., Jan 18 2008, In : Biosensors and Bioelectronics. 23, 6, p. 919-923 5 p.

Research output: Contribution to journalArticle

Lipid bilayers
Lipid Bilayers
Membrane Lipids
Ion Channels
Membranes
2007
23 Citations (Scopus)

Disulfide trapping the mechanosensitive channel MscL into a gating-transition state

Iscla, I., Levin, G., Wray, R. & Blount, P., Feb 2007, In : Biophysical Journal. 92, 4, p. 1224-1232 9 p.

Research output: Contribution to journalArticle

Disulfides
Cysteine
Site-Directed Mutagenesis
Emergencies
Bacteria
18 Citations (Scopus)

MscL: The Bacterial Mechanosensitive Channel of Large Conductance

Blount, P., Iscla, I., Moe, P. C. & Li, Y., 2007, In : Current Topics in Membranes. 58, p. 201-233 33 p.

Research output: Contribution to journalArticle

2006
26 Citations (Scopus)

Mechanosensitive channel gating transitions resolved by functional changes upon pore modification

Bartlett, J. L., Li, Y. & Blount, P., Nov 2006, In : Biophysical Journal. 91, 10, p. 3684-3691 8 p.

Research output: Contribution to journalArticle

Sulfhydryl Reagents
Structural Models
Molecular Dynamics Simulation
Cysteine
Shock
48 Citations (Scopus)
Epithelial Sodium Channels
Phosphorylation
Colforsin
Oocytes
Mutagenesis
2005
125 Citations (Scopus)

Assessment of potential stimuli for mechano-dependent gating of MscL: Effects of pressure, tension, and lipid headgroups

Moe, P. & Blount, P., Sep 13 2005, In : Biochemistry. 44, 36, p. 12239-12244 6 p.

Research output: Contribution to journalArticle

Membranes
Lipids
Pressure
Pressure relief valves
Lipid bilayers
29 Citations (Scopus)

Lactococcus lactis uses MscL as its principal mechanosensitive channel

Folgering, J. H. A., Moe, P. C., Schuurman-Wolters, G. K., Blount, P. & Poolman, B., Mar 11 2005, In : Journal of Biological Chemistry. 280, 10, p. 8784-8792 9 p.

Research output: Contribution to journalArticle

Betaine
Lactococcus lactis
Genes
Membranes
Escherichia coli
97 Citations (Scopus)

Pivotal role of the glycine-rich TM3 helix in gating the MscS mechanosensitive channel

Edwards, M. D., Li, Y., Kim, S., Miller, S., Bartlett, W., Black, S., Dennison, S., Iscla, I., Blount, P., Bowie, J. U. & Booth, I. R., Feb 20 2005, In : Nature Structural and Molecular Biology. 12, 2, p. 113-119 7 p.

Research output: Contribution to journalArticle

Glycine
Alanine
Pressure
Structural Models
Escherichia coli
15 Citations (Scopus)

Selective serotonin reuptake inhibitors induce spontaneous interneuronal activity in the leech nervous system

Calviño, M. A., Iscla, I. R. & Szczupak, L., May 2005, In : Journal of Neurophysiology. 93, 5, p. 2644-2655 12 p.

Research output: Contribution to journalArticle

Leeches
Serotonin Uptake Inhibitors
Nervous System
Serotonin
Fluoxetine
2004
44 Citations (Scopus)

An in vivo assay identifies changes in residue accessibility on mechanosensitive channel gating

Bartlett, J. L., Levin, G. & Blount, P., Jul 6 2004, In : Proceedings of the National Academy of Sciences of the United States of America. 101, 27, p. 10161-10165 5 p.

Research output: Contribution to journalArticle

Sulfhydryl Reagents
Mycobacterium tuberculosis
Cysteine
Shock
Cell Survival
33 Citations (Scopus)

Channels in microbes: So many holes to fill

Kung, C. & Blount, P., Jul 2004, In : Molecular Microbiology. 53, 2, p. 373-380 8 p.

Research output: Contribution to journalArticle

Neurobiology
Intelligence
Ion Channels
Hot Temperature
Genome
48 Citations (Scopus)

Cysteine Scanning of MscL Transmembrane Domains Reveals Residues Critical for Mechanosensitive Channel Gating

Levin, G. & Blount, P., May 2004, In : Biophysical Journal. 86, 5, p. 2862-2870 9 p.

Research output: Contribution to journalArticle

Cysteine
Phenotype
Crystallography
Structural Models
Mycobacterium tuberculosis
34 Citations (Scopus)

Defining the physical gate of a mechanosensitive channel, MscL, by engineering metal-binding sites

Iscla, I., Levin, G., Wray, R., Reynolds, R. & Blount, P., Nov 2004, In : Biophysical Journal. 87, 5, p. 3172-3180 9 p.

Research output: Contribution to journalArticle

Metals
Binding Sites
Mycobacterium tuberculosis
Histidine
Ions
23 Citations (Scopus)

Intragenic suppression of gain-of-function mutations in the Escherichia coli mechanosensitive channel, MscL

Li, Y., Wray, R. & Blount, P., Jul 2004, In : Molecular Microbiology. 53, 2, p. 485-495 11 p.

Research output: Contribution to journalArticle

Escherichia coli
Mutation
Growth
Phenotype
Mutagenesis
7 Citations (Scopus)

Modulation of Mechanosensory Responses by Motoneurons That Regulate Skin Surface Topology in the Leech

Rodriguez, M. J., Iscla, I. R. & Szczupak, L., May 2004, In : Journal of Neurophysiology. 91, 5, p. 2366-2375 10 p.

Research output: Contribution to journalArticle

Leeches
Motor Neurons
Skin
T-Lymphocytes
Ganglia
2003
1 Citation (Scopus)

Expression of macrophage inflammatory protein 1 alpha in the endothelial cells exposed to diamide.

Yang, L., Zhu, X., Zhao, X. & Deng, Z., 2003, Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. 3 ed. Vol. 23. p. 219-222, 233

Research output: Chapter in Book/Report/Conference proceedingChapter

Diamide
Chemokine CCL3
Macrophages
Endothelial cells
Endothelial Cells
7 Citations (Scopus)

Inhibition of magnesium lithospermate B on the c-Jun N-terminal kinase 3 mRNA expression in cardiomyocytes encountered ischemia/reperfusion injury

Yang, L. M., Xiao, Y. L. & Ou-Yang, J. H., Jul 2003, In : Yaoxue Xuebao. 38, 7, p. 487-491 5 p.

Research output: Contribution to journalArticle

Mitogen-Activated Protein Kinase 10
Reperfusion Injury
Cardiac Myocytes
Messenger RNA
Reperfusion
1 Citation (Scopus)
Macrophage Inflammatory Proteins
Human Umbilical Vein Endothelial Cells
Lipopolysaccharides
Messenger RNA
Control Groups
31 Citations (Scopus)

Molecular Mechanisms of Mechanosensation: Big Lessons from Small Cells

Blount, P., Mar 6 2003, In : Neuron. 37, 5, p. 731-734 4 p.

Research output: Contribution to journalArticle

Bacterial Structures
Membrane Proteins
Bacteria
Lipids
2002
55 Citations (Scopus)

Family ties of gated pores: Evolution of the sensor module

Kumánovics, A., Levin, G. & Blount, P., Oct 2002, In : FASEB Journal. 16, 12, p. 1623-1629 7 p.

Research output: Contribution to journalArticle

TRPP Cation Channels
Transient Receptor Potential Channels
Membrane Lipids
sensors (equipment)
Phenotype
67 Citations (Scopus)

Functional design of bacterial mechanosensitive channels. Comparisons and contrasts illuminated by random mutagenesis

Okada, K., Moe, P. C. & Blount, P., Aug 2 2002, In : Journal of Biological Chemistry. 277, 31, p. 27682-27688 7 p.

Research output: Contribution to journalArticle

Mutagenesis
Genes
Phenotype
Mutation
Cell membranes