Physiology - Division D

Research Output

2020
Open Access
Open Access
2019

An agonist of the MscL channel affects multiple bacterial species and increases membrane permeability and potency of common antibiotics

Wray, R., Herrera, N., Iscla, I., Wang, J. & Blount, P., Sep 1 2019, In : Molecular Microbiology. 112, 3, p. 896-905 10 p.

Research output: Contribution to journalArticle

2 Scopus citations
1 Scopus citations

Interaction of the Mechanosensitive Channel, MscS, with the Membrane Bilayer through Lipid Intercalation into Grooves and Pockets

Rasmussen, T., Rasmussen, A., Yang, L., Kaul, C., Black, S., Galbiati, H., Conway, S. J., Miller, S., Blount, P. & Booth, I. R., Aug 9 2019, In : Journal of Molecular Biology. 431, 17, p. 3339-3352 14 p.

Research output: Contribution to journalArticle

Open Access
1 Scopus citations

Novel compounds that specifically bind and modulate MscL: insights into channel gating mechanisms

Wray, R., Iscla, I., Kovacs, Z., Wang, J. & Blount, P., Mar 1 2019, In : FASEB Journal. 33, 3, p. 3180-3189 10 p.

Research output: Contribution to journalArticle

Open Access
2 Scopus citations
2018

Effects of Low Intensity Focused Ultrasound on Liposomes Containing Channel proteins

Babakhanian, M., Yang, L., Nowroozi, B., Saddik, G., Boodaghians, L., Blount, P. & Grundfest, W., Nov 22 2018, In : Scientific Reports. 8, 1, 1 p.

Research output: Contribution to journalArticle

4 Scopus citations

Engineering a pH-Sensitive Liposomal MRI Agent by Modification of a Bacterial Channel

Yang, L. M., Zheng, H., Ratnakar, J. S., Adebesin, B. Y., Do, Q. N., Kovacs, Z. & Blount, P., Jan 1 2018, (Accepted/In press) In : Small.

Research output: Contribution to journalArticle

6 Scopus citations

The phosphorylation site T613 in the β-subunit of rat epithelial Na+ channel (ENaC) modulates channel inhibition by Nedd4-2

Krueger, B., Yang, L., Korbmacher, C. & Rauh, R., Apr 1 2018, In : Pflugers Archiv European Journal of Physiology. 470, 4, p. 649-660 12 p.

Research output: Contribution to journalArticle

4 Scopus citations
2016

Dihydrostreptomycin Directly Binds to, Modulates, and Passes through the MscL Channel Pore

Wray, R., Iscla, I., Gao, Y., Li, H., Wang, J. & Blount, P., Jun 9 2016, In : PLoS Biology. 14, 6, e1002473.

Research output: Contribution to journalArticle

9 Scopus citations
2015

A new antibiotic with potent activity targets MscL

Iscla, I., Wray, R., Blount, P., Larkins-Ford, J., Conery, A. L., Ausubel, F. M., Ramu, S., Kavanagh, A., Huang, J. X., Blaskovich, M. A., Cooper, M. A., Obregon-Henao, A., Orme, I., Tjandra, E. S., Stroeher, U. H., Brown, M. H., Macardle, C., Van Holst, N., Ling Tong, C., Slattery, A. D. & 3 others, Gibson, C. T., Raston, C. L. & Boulos, R. A., Jul 29 2015, In : Journal of Antibiotics. 68, 7, p. 453-462 10 p.

Research output: Contribution to journalArticle

20 Scopus citations

Mutations in a conserved domain of E. coli MscS to the most conserved superfamily residue leads to kinetic changes

Malcolm, H. R. & Blount, P., Sep 4 2015, In : PLoS One. 10, 9, e0136756.

Research output: Contribution to journalArticle

6 Scopus citations

Scanning MscL channels with targeted post-translational modifications for functional alterations

Iscla, I., Wray, R., Eaton, C. & Blount, P., Sep 14 2015, In : PLoS One. 10, 9, e0137994.

Research output: Contribution to journalArticle

11 Scopus citations

The mechanosensitive channel of small conductance (MscS) functions as a Jack-in-the box

Malcolm, H. R., Blount, P. & Maurer, J. A., Jan 1 2015, In : Biochimica et Biophysica Acta - General Subjects. 1848, 1 Pt A, p. 159-166 8 p.

Research output: Contribution to journalArticle

18 Scopus citations
2014

Dynamics of protein-protein interactions at the mscl periplasmic-lipid interface

Zhong, D., Yang, L. M. & Blount, P., Jan 21 2014, In : Biophysical Journal. 106, 2, p. 375-381 7 p.

Research output: Contribution to journalArticle

5 Scopus citations

Electrostatics at the membrane define MscL channel mechanosensitivity and kinetics

Zhong, D. & Blount, P., Dec 1 2014, In : FASEB Journal. 28, 12, p. 5234-5241 8 p.

Research output: Contribution to journalArticle

4 Scopus citations

Streptomycin potency is dependent on MscL channel expression

Iscla, I., Wray, R., Wei, S., Posner, B. & Blount, P., 2014, In : Nature communications. 5, 4891.

Research output: Contribution to journalArticle

24 Scopus citations
2013

Chimeras Reveal a Single Lipid-Interface Residue that Controls MscL Channel Kinetics as well as Mechanosensitivity

Yang, L. M., Zhong, D. & Blount, P., Feb 15 2013, In : Cell Reports. 3, 2, p. 520-527 8 p.

Research output: Contribution to journalArticle

8 Scopus citations

Improving the design of a MscL-based triggered nanovalve

Iscla, I., Eaton, C., Parker, J., Wray, R., Kovács, Z. & Blount, P., Mar 2013, In : Biosensors. 3, 1, p. 171-184 14 p.

Research output: Contribution to journalArticle

21 Scopus citations

Phosphatidylinositol is crucial for the mechanosensitivity of mycobacterium tuberculosis MscL

Zhong, D. & Blount, P., Aug 13 2013, In : Biochemistry. 52, 32, p. 5415-5420 6 p.

Research output: Contribution to journalArticle

19 Scopus citations
2012

Sensing and responding to membrane tension: The bacterial MscL channel as a model system

Iscla, I. & Blount, P., Jul 18 2012, In : Biophysical Journal. 103, 2, p. 169-174 6 p.

Research output: Contribution to journalReview article

43 Scopus citations

Structure and molecular mechanism of an anion-selective mechanosensitive channel of small conductance

Zhang, X., Wang, J., Feng, Y., Ge, J., Li, W., Sun, W., Iscla, I., Yu, J., Blount, P., Li, Y. & Yang, M., Oct 30 2012, In : Proceedings of the National Academy of Sciences of the United States of America. 109, 44, p. 18180-18185 6 p.

Research output: Contribution to journalArticle

37 Scopus citations

The dynamics of protein-protein interactions between domains of MscL at the cytoplasmic-lipid interface

Iscla, I., Wray, R. & Blount, P., Jan 1 2012, In : Channels. 6, 4

Research output: Contribution to journalArticle

7 Scopus citations

The MscS and MscL families of mechanosensitive channels act as microbial emergency release valves

Bootha, I. R. & Blount, P., Sep 1 2012, In : Journal of bacteriology. 194, 18, p. 4802-4809 8 p.

Research output: Contribution to journalShort survey

97 Scopus citations

Three routes to modulate the pore size of the Mscl channel/nanovalve

Yang, L. M., Wray, R., Parker, J., Wilson, D., Duran, R. S. & Blount, P., Feb 28 2012, In : ACS Nano. 6, 2, p. 1134-1141 8 p.

Research output: Contribution to journalArticle

26 Scopus citations
2011

An in vivo screen reveals protein-lipid interactions crucial for gating a mechanosensitive channel

Iscla, I., Wray, R. & Blount, P., Feb 1 2011, In : FASEB Journal. 25, 2, p. 694-702 9 p.

Research output: Contribution to journalArticle

18 Scopus citations

Manipulating the permeation of charged compounds through the MscL nanovalve

Yang, L. M. & Blount, P., Jan 1 2011, In : FASEB Journal. 25, 1, p. 428-434 7 p.

Research output: Contribution to journalArticle

12 Scopus citations

The oligomeric state of the truncated mechanosensitive channel of large conductance shows no variance in vivo

Iscla, I., Wray, R. & Blount, P., Sep 1 2011, In : Protein Science. 20, 9, p. 1638-1642 5 p.

Research output: Contribution to journalArticle

28 Scopus citations
2010
48 Scopus citations
2009

An open-pore structure of the mechanosensitive channel MscL derived by determining transmembrane domain interactions upon gating

Li, Y., Wray, R., Eaton, C. & Blount, P., Jul 1 2009, In : FASEB Journal. 23, 7, p. 2197-2204 8 p.

Research output: Contribution to journalArticle

24 Scopus citations

Cholesterol depletion of the plasma membrane prevents activation of the epithelial sodium channel (ENaC) by SGK1

Krueger, B., Haerteis, S., Yang, L., Hartner, A., Rauh, R., Korbmacher, C. & Diakov, A., Jan 1 2009, In : Cellular Physiology and Biochemistry. 24, 5-6, p. 605-618 14 p.

Research output: Contribution to journalArticle

42 Scopus citations
2008

On the structure of the N-terminal domain of the MscL channel: Helical bundle or membrane interface

Iscla, I., Wray, R. & Blount, P., Sep 1 2008, In : Biophysical Journal. 95, 5, p. 2283-2291 9 p.

Research output: Contribution to journalArticle

47 Scopus citations

Voltage-induced gating of the mechanosensitive MscL ion channel reconstituted in a tethered lipid bilayer membrane

Andersson, M., Okeyo, G., Wilson, D., Keizer, H., Moe, P., Blount, P., Fine, D., Dodabalapur, A. & Duran, R. S., Jan 18 2008, In : Biosensors and Bioelectronics. 23, 6, p. 919-923 5 p.

Research output: Contribution to journalArticle

31 Scopus citations
2007

Disulfide trapping the mechanosensitive channel MscL into a gating-transition state

Iscla, I., Levin, G., Wray, R. & Blount, P., Feb 2007, In : Biophysical journal. 92, 4, p. 1224-1232 9 p.

Research output: Contribution to journalArticle

25 Scopus citations

MscL: The Bacterial Mechanosensitive Channel of Large Conductance

Blount, P., Iscla, I., Moe, P. C. & Li, Y., 2007, In : Current Topics in Membranes. 58, p. 201-233 33 p.

Research output: Contribution to journalArticle

18 Scopus citations
2006

Mechanosensitive channel gating transitions resolved by functional changes upon pore modification

Bartlett, J. L., Li, Y. & Blount, P., Nov 2006, In : Biophysical journal. 91, 10, p. 3684-3691 8 p.

Research output: Contribution to journalArticle

29 Scopus citations
49 Scopus citations
2005

Assessment of potential stimuli for mechano-dependent gating of MscL: Effects of pressure, tension, and lipid headgroups

Moe, P. & Blount, P., Sep 13 2005, In : Biochemistry. 44, 36, p. 12239-12244 6 p.

Research output: Contribution to journalArticle

130 Scopus citations

Lactococcus lactis uses MscL as its principal mechanosensitive channel

Folgering, J. H. A., Moe, P. C., Schuurman-Wolters, G. K., Blount, P. & Poolman, B., Mar 11 2005, In : Journal of Biological Chemistry. 280, 10, p. 8784-8792 9 p.

Research output: Contribution to journalArticle

30 Scopus citations

Pivotal role of the glycine-rich TM3 helix in gating the MscS mechanosensitive channel

Edwards, M. D., Li, Y., Kim, S., Miller, S., Bartlett, W., Black, S., Dennison, S., Iscla, I., Blount, P., Bowie, J. U. & Booth, I. R., Feb 20 2005, In : Nature Structural and Molecular Biology. 12, 2, p. 113-119 7 p.

Research output: Contribution to journalArticle

99 Scopus citations

Selective serotonin reuptake inhibitors induce spontaneous interneuronal activity in the leech nervous system

Calviño, M. A., Iscla, I. R. & Szczupak, L., May 1 2005, In : Journal of neurophysiology. 93, 5, p. 2644-2655 12 p.

Research output: Contribution to journalArticle

15 Scopus citations
2004

An in vivo assay identifies changes in residue accessibility on mechanosensitive channel gating

Bartlett, J. L., Levin, G. & Blount, P., Jul 6 2004, In : Proceedings of the National Academy of Sciences of the United States of America. 101, 27, p. 10161-10165 5 p.

Research output: Contribution to journalArticle

47 Scopus citations

Channels in microbes: So many holes to fill

Kung, C. & Blount, P., Jul 1 2004, In : Molecular Microbiology. 53, 2, p. 373-380 8 p.

Research output: Contribution to journalShort survey

33 Scopus citations

Cysteine Scanning of MscL Transmembrane Domains Reveals Residues Critical for Mechanosensitive Channel Gating

Levin, G. & Blount, P., May 2004, In : Biophysical journal. 86, 5, p. 2862-2870 9 p.

Research output: Contribution to journalArticle

Open Access
51 Scopus citations

Defining the physical gate of a mechanosensitive channel, MscL, by engineering metal-binding sites

Iscla, I., Levin, G., Wray, R., Reynolds, R. & Blount, P., Nov 2004, In : Biophysical journal. 87, 5, p. 3172-3180 9 p.

Research output: Contribution to journalArticle

34 Scopus citations

Intragenic suppression of gain-of-function mutations in the Escherichia coli mechanosensitive channel, MscL

Li, Y., Wray, R. & Blount, P., Jul 1 2004, In : Molecular Microbiology. 53, 2, p. 485-495 11 p.

Research output: Contribution to journalArticle

24 Scopus citations

Modulation of Mechanosensory Responses by Motoneurons That Regulate Skin Surface Topology in the Leech

Rodriguez, M. J., Iscla, I. R. & Szczupak, L., May 1 2004, In : Journal of neurophysiology. 91, 5, p. 2366-2375 10 p.

Research output: Contribution to journalArticle

8 Scopus citations
2003

Expression of macrophage inflammatory protein 1 alpha in the endothelial cells exposed to diamide.

Yang, L., Zhu, X., Zhao, X. & Deng, Z., 2003, Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. 3 ed. Vol. 23. p. 219-222, 233

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Inhibition of magnesium lithospermate B on the c-Jun N-terminal kinase 3 mRNA expression in cardiomyocytes encountered ischemia/reperfusion injury

Yang, L. M., Xiao, Y. L. & Ou-Yang, J. H., Jul 1 2003, In : Yaoxue Xuebao. 38, 7, p. 487-491 5 p.

Research output: Contribution to journalArticle

7 Scopus citations