@article{c8ae545e81e947359488462a335e472f,
title = "βKslotho is required for fibroblast growth factor 21 effects on growth and metabolism",
abstract = "Fibroblast growth factor 21 (FGF21) is a fasting-induced hepatokine that has potent pharmacologic effects in mice, which include improving insulin sensitivity and blunting growth. The single-transmembrane protein βKlotho functions as a coreceptor for FGF21 in vitro. To determine if βKlotho is required for FGF21 action in vivo, we generated whole-body and adipose tissue-selective βKlotho-knockout mice. All of the effects of FGF21 on growth and metabolism were lost in whole-body βKlotho-knockout mice. Selective elimination of βKlotho in adipose tissue blocked the acute insulin-sensitizing effects of FGF21. Taken together, these data demonstrate that βKlotho is essential for FGF21 activity and that βKlotho in adipose tissue contributes to the beneficial metabolic actions of FGF21.",
author = "Xunshan Ding and Jamie Boney-Montoya and Owen, {Bryn M.} and Bookout, {Angie L.} and Coate, {Katie Colbert} and Mangelsdorf, {David J.} and Kliewer, {Steven A.}",
note = "Funding Information: We thank Yuan Zhang, Kevin Vale, Heather Lawrence, Li Peng, and Vicky Lin for assistance with animal experiments; Yihong Wan for bone measurements and bone-marrow-derived and spleen-derived macrophage cDNA samples; Michihisa Umetani for peritoneal macrophage cDNA samples; Joseph Takahashi for providing CamK2a-Cre mice; Mi Hwa Kim for help with the clamp study; and Moosa Mohammadi and Regina Goetz for providing recombinant FGF21 protein. This work was supported by National Institutes of Health grants DK067158, P20RR20691, and 1RL1GM084436-01 (to S.A.K. and D.J.M.), U19DK62434 (to D.J.M.), and GM007062 (A.L.B.); the Robert A. Welch Foundation (grant I-1558 to S.A.K., grant I-1275 to D.J.M.); and the Howard Hughes Medical Institute (to D.J.M.). ",
year = "2012",
month = sep,
day = "5",
doi = "10.1016/j.cmet.2012.08.002",
language = "English (US)",
volume = "16",
pages = "387--393",
journal = "Cell Metabolism",
issn = "1550-4131",
publisher = "Cell Press",
number = "3",
}