TY - JOUR
T1 - γδ T cells are required for maximal expression of allergic conjunctivitis
AU - Reyes, Nancy J.
AU - Mayhew, Elizabeth
AU - Chen, Peter W.
AU - Niederkorn, Jerry Y.
PY - 2011/4
Y1 - 2011/4
N2 - PURPOSE. To determine the function of γδ T cells in early- and late-phase responses in allergic conjunctivitis. METHODS. Wild-type (WT) C57BL/6 and γδ T cell- deficient (TCR-δ-/-) mice were immunized intraperitoneally and challenged topically for 7 consecutive days with short ragweed pollen. Natural killer T (NKT) and γδ T cell- double-deficient mice were generated by treating TCR-δ-/- mice with anti- CD1d antibody. Allergic conjunctivitis was evaluated clinically, and the late-phase response was assessed by histopathology. Cytokine profiles were evaluated by ELISA. The afferent and efferent arms of allergic conjunctivitis were assessed by adoptive transfer of CD4+ T cells from WT or TCR-δ-/- mice into naive TCR-δ-/- or WT mice. RESULTS. TCR-δ-/- mice had decreased clinical manifestations of allergic conjunctivitis compared with WT mice. TCR-δ-/- mice had decreased eosinophilic infiltration compared with WT mice. TCR-δ-/- mice produced less Th2-associated cytokines interleukin (IL)-4, -5, and -13 compared with WT mice. Clinical manifestations of allergic conjunctivitis were lowest in NKT cell- depleted TCR-δ-/- mice. However, late-phase allergic conjunctivitis in NKT cell- depleted, TCR-δ-/- mice was the same as TCR-δ-/- mice. Adoptive transfer of CD4+ T cells revealed that γδ T cells are needed for the afferent and efferent arms of allergic conjunctivitis. CONCLUSIONS. γδ T cells are needed for full expression of both the clinical manifestations and the late phase of allergic conjunctivitis. Thus, γδ T cells have an important impact in the expression of allergic conjunctivitis and are a potential therapeutic target in the management of allergic diseases of the ocular surface.
AB - PURPOSE. To determine the function of γδ T cells in early- and late-phase responses in allergic conjunctivitis. METHODS. Wild-type (WT) C57BL/6 and γδ T cell- deficient (TCR-δ-/-) mice were immunized intraperitoneally and challenged topically for 7 consecutive days with short ragweed pollen. Natural killer T (NKT) and γδ T cell- double-deficient mice were generated by treating TCR-δ-/- mice with anti- CD1d antibody. Allergic conjunctivitis was evaluated clinically, and the late-phase response was assessed by histopathology. Cytokine profiles were evaluated by ELISA. The afferent and efferent arms of allergic conjunctivitis were assessed by adoptive transfer of CD4+ T cells from WT or TCR-δ-/- mice into naive TCR-δ-/- or WT mice. RESULTS. TCR-δ-/- mice had decreased clinical manifestations of allergic conjunctivitis compared with WT mice. TCR-δ-/- mice had decreased eosinophilic infiltration compared with WT mice. TCR-δ-/- mice produced less Th2-associated cytokines interleukin (IL)-4, -5, and -13 compared with WT mice. Clinical manifestations of allergic conjunctivitis were lowest in NKT cell- depleted TCR-δ-/- mice. However, late-phase allergic conjunctivitis in NKT cell- depleted, TCR-δ-/- mice was the same as TCR-δ-/- mice. Adoptive transfer of CD4+ T cells revealed that γδ T cells are needed for the afferent and efferent arms of allergic conjunctivitis. CONCLUSIONS. γδ T cells are needed for full expression of both the clinical manifestations and the late phase of allergic conjunctivitis. Thus, γδ T cells have an important impact in the expression of allergic conjunctivitis and are a potential therapeutic target in the management of allergic diseases of the ocular surface.
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U2 - 10.1167/iovs.10-5959
DO - 10.1167/iovs.10-5959
M3 - Article
C2 - 21212171
AN - SCOPUS:79956002909
SN - 0146-0404
VL - 52
SP - 2211
EP - 2216
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 5
ER -