Abstract
We have used highly specific affinity-purified antibodies against mouse κ- and λ-chains in conjunction with both immunofluorescence analysis on the FACS and enzymatic radioiodination of murine splenocytes and bone marrow cells. Our results indicate that the κ/λ ratio of cell surface immunoglobulin is constant during the ontogenetic development of B cells, i.e., a shift in the κ/λ ratio does not occur during B cell maturation. Our results suggest that the events controlling κ and λ expression on murine B cells are decided very early in ontogeny, before significant antigenic selection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1670-1673 |
| Number of pages | 4 |
| Journal | Journal of Immunology |
| Volume | 127 |
| Issue number | 4 |
| State | Published - Jan 1 1981 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology