α-Fetoprotein inhibits macrophage expression of Ia antigens

C. Y. Lu, P. S. Changelian, E. R. Unanue

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Murine α-fetoprotein (AFP) is a major protein in amnionic fluid and perinatal sera. AFP has been postulatd to contribute to the immunologic hyporesponsiveness of the fetus and neonate, as well as protecting the fetus from rejection by the mother. We now report that AFP acts in vitro to inhibit macrophage expression of cell surface Ia antigens, the class II glycoproteins of the major histocompatibility gene complex. In culture, macrophages incubated with a T cell lymphokine developed Ia as detected by either immunofluorescence or a cell radioimmunoassay. Both mouse amnionic fluid and AFP inhibited the expression of Ia in a dose-dependent manner. Five preparations of AFP derived from three sources - day-old neonates, amnionic fluid, and a hepatoma cell line - were effective. The concentration of AFP that inhibited by 50% the expression of Ia was about 10-6 M. Mouse amnionic fluid and AFP did not affect the viability of the macrophage nor was the surface expression of H-2K and C3 receptors affected. The inhibitory effect of AFP did not depend on the presence of T cells in the culture. AFP did not appear to inhibit the direct interaction of lymphokine with macrophages; AFP did inhibit if given days after a pulse of lymphokine. The concentrations of prostaglandins carried by AFP were insignificant and could not explain the inhibitory effects.

Original languageEnglish (US)
Pages (from-to)1722-1727
Number of pages6
JournalJournal of Immunology
Volume132
Issue number4
StatePublished - 1984

Fingerprint

Fetal Proteins
Histocompatibility Antigens Class II
Lymphokines
Macrophages
Fetus
T-Lymphocytes
Surface Antigens
Major Histocompatibility Complex
Prostaglandins
Radioimmunoassay
Fluorescent Antibody Technique
Hepatocellular Carcinoma
Glycoproteins
Cell Culture Techniques
Cell Line
Serum
Genes
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Lu, C. Y., Changelian, P. S., & Unanue, E. R. (1984). α-Fetoprotein inhibits macrophage expression of Ia antigens. Journal of Immunology, 132(4), 1722-1727.

α-Fetoprotein inhibits macrophage expression of Ia antigens. / Lu, C. Y.; Changelian, P. S.; Unanue, E. R.

In: Journal of Immunology, Vol. 132, No. 4, 1984, p. 1722-1727.

Research output: Contribution to journalArticle

Lu, CY, Changelian, PS & Unanue, ER 1984, 'α-Fetoprotein inhibits macrophage expression of Ia antigens', Journal of Immunology, vol. 132, no. 4, pp. 1722-1727.
Lu, C. Y. ; Changelian, P. S. ; Unanue, E. R. / α-Fetoprotein inhibits macrophage expression of Ia antigens. In: Journal of Immunology. 1984 ; Vol. 132, No. 4. pp. 1722-1727.
@article{10cf425658854eee89651f6bf46b8136,
title = "α-Fetoprotein inhibits macrophage expression of Ia antigens",
abstract = "Murine α-fetoprotein (AFP) is a major protein in amnionic fluid and perinatal sera. AFP has been postulatd to contribute to the immunologic hyporesponsiveness of the fetus and neonate, as well as protecting the fetus from rejection by the mother. We now report that AFP acts in vitro to inhibit macrophage expression of cell surface Ia antigens, the class II glycoproteins of the major histocompatibility gene complex. In culture, macrophages incubated with a T cell lymphokine developed Ia as detected by either immunofluorescence or a cell radioimmunoassay. Both mouse amnionic fluid and AFP inhibited the expression of Ia in a dose-dependent manner. Five preparations of AFP derived from three sources - day-old neonates, amnionic fluid, and a hepatoma cell line - were effective. The concentration of AFP that inhibited by 50{\%} the expression of Ia was about 10-6 M. Mouse amnionic fluid and AFP did not affect the viability of the macrophage nor was the surface expression of H-2K and C3 receptors affected. The inhibitory effect of AFP did not depend on the presence of T cells in the culture. AFP did not appear to inhibit the direct interaction of lymphokine with macrophages; AFP did inhibit if given days after a pulse of lymphokine. The concentrations of prostaglandins carried by AFP were insignificant and could not explain the inhibitory effects.",
author = "Lu, {C. Y.} and Changelian, {P. S.} and Unanue, {E. R.}",
year = "1984",
language = "English (US)",
volume = "132",
pages = "1722--1727",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "4",

}

TY - JOUR

T1 - α-Fetoprotein inhibits macrophage expression of Ia antigens

AU - Lu, C. Y.

AU - Changelian, P. S.

AU - Unanue, E. R.

PY - 1984

Y1 - 1984

N2 - Murine α-fetoprotein (AFP) is a major protein in amnionic fluid and perinatal sera. AFP has been postulatd to contribute to the immunologic hyporesponsiveness of the fetus and neonate, as well as protecting the fetus from rejection by the mother. We now report that AFP acts in vitro to inhibit macrophage expression of cell surface Ia antigens, the class II glycoproteins of the major histocompatibility gene complex. In culture, macrophages incubated with a T cell lymphokine developed Ia as detected by either immunofluorescence or a cell radioimmunoassay. Both mouse amnionic fluid and AFP inhibited the expression of Ia in a dose-dependent manner. Five preparations of AFP derived from three sources - day-old neonates, amnionic fluid, and a hepatoma cell line - were effective. The concentration of AFP that inhibited by 50% the expression of Ia was about 10-6 M. Mouse amnionic fluid and AFP did not affect the viability of the macrophage nor was the surface expression of H-2K and C3 receptors affected. The inhibitory effect of AFP did not depend on the presence of T cells in the culture. AFP did not appear to inhibit the direct interaction of lymphokine with macrophages; AFP did inhibit if given days after a pulse of lymphokine. The concentrations of prostaglandins carried by AFP were insignificant and could not explain the inhibitory effects.

AB - Murine α-fetoprotein (AFP) is a major protein in amnionic fluid and perinatal sera. AFP has been postulatd to contribute to the immunologic hyporesponsiveness of the fetus and neonate, as well as protecting the fetus from rejection by the mother. We now report that AFP acts in vitro to inhibit macrophage expression of cell surface Ia antigens, the class II glycoproteins of the major histocompatibility gene complex. In culture, macrophages incubated with a T cell lymphokine developed Ia as detected by either immunofluorescence or a cell radioimmunoassay. Both mouse amnionic fluid and AFP inhibited the expression of Ia in a dose-dependent manner. Five preparations of AFP derived from three sources - day-old neonates, amnionic fluid, and a hepatoma cell line - were effective. The concentration of AFP that inhibited by 50% the expression of Ia was about 10-6 M. Mouse amnionic fluid and AFP did not affect the viability of the macrophage nor was the surface expression of H-2K and C3 receptors affected. The inhibitory effect of AFP did not depend on the presence of T cells in the culture. AFP did not appear to inhibit the direct interaction of lymphokine with macrophages; AFP did inhibit if given days after a pulse of lymphokine. The concentrations of prostaglandins carried by AFP were insignificant and could not explain the inhibitory effects.

UR - http://www.scopus.com/inward/record.url?scp=0021323770&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021323770&partnerID=8YFLogxK

M3 - Article

C2 - 6199409

AN - SCOPUS:0021323770

VL - 132

SP - 1722

EP - 1727

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -