α-MSH production, receptors, and influence on neopterin in a human monocyte/macrophage cell line

N. Rajora, G. Ceriani, A. Catania, R. A. Star, M. T. Murphy, J. M. Lipton

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

α-Melanocyte-stimulating hormone (α-MSH), a tridecapeptide derived from proopiomelanocortin, has potent antiinflammatory activity in laboratory animals, α-MSH inhibits nitric oxide production by murine macrophages, an influence believed to reflect activation of an autocrine circuit in these cells, one that is based on production and release of α-RISH and subsequent stimulation of melanocortin receptors. We found that THP-1 cells, human monocytic cells, produced α-MSB; this production was increased by interleukin-6, tumor necrosis factor α or concanavalin A. These cells also expressed the gene for the human α-MSH receptor MC1. Unlike murine macrophages, TMP-1 cells produced little nitrite in response to interferon-γ (IFN-γ) and lipopolysaccharide, and α-MSH inhibited this production only slightly. However, production of neopterin, a presumed primate homologue of nitric oxide in lower animals, was increased in THIP-1 cells stimulated with IFN-γ plus TNF-α and α-RLSH significantly inhibited this production. The evidence indicates that an autocrine regulatory circuit based on α-MSH occurs in human monocyte/macrophages much as in murine macrophages. α-MSH-induced modulation of specific inflammatory mediators/cytotoxic agents appears to differ depending on the importance of the mediators in the myelomonocytic cells of different species.

Original languageEnglish (US)
Pages (from-to)248-253
Number of pages6
JournalJournal of Leukocyte Biology
Volume59
Issue number2
StatePublished - Feb 1996

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Melanocyte-Stimulating Hormones
Neopterin
Monocytes
Macrophages
Cell Line
Interferons
Nitric Oxide
Melanocortin Receptors
Thymidine Monophosphate
Pro-Opiomelanocortin
Cytotoxins
Laboratory Animals
Concanavalin A
Nitrites
Primates
Lipopolysaccharides
Interleukin-6
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha

Keywords

  • Autocrine regulations
  • IL-6
  • Inflammation
  • Melanocortin receptors
  • Nitric oxide
  • TNF

ASJC Scopus subject areas

  • Cell Biology

Cite this

Rajora, N., Ceriani, G., Catania, A., Star, R. A., Murphy, M. T., & Lipton, J. M. (1996). α-MSH production, receptors, and influence on neopterin in a human monocyte/macrophage cell line. Journal of Leukocyte Biology, 59(2), 248-253.

α-MSH production, receptors, and influence on neopterin in a human monocyte/macrophage cell line. / Rajora, N.; Ceriani, G.; Catania, A.; Star, R. A.; Murphy, M. T.; Lipton, J. M.

In: Journal of Leukocyte Biology, Vol. 59, No. 2, 02.1996, p. 248-253.

Research output: Contribution to journalArticle

Rajora, N, Ceriani, G, Catania, A, Star, RA, Murphy, MT & Lipton, JM 1996, 'α-MSH production, receptors, and influence on neopterin in a human monocyte/macrophage cell line', Journal of Leukocyte Biology, vol. 59, no. 2, pp. 248-253.
Rajora, N. ; Ceriani, G. ; Catania, A. ; Star, R. A. ; Murphy, M. T. ; Lipton, J. M. / α-MSH production, receptors, and influence on neopterin in a human monocyte/macrophage cell line. In: Journal of Leukocyte Biology. 1996 ; Vol. 59, No. 2. pp. 248-253.
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