β-2-glycoprotein 1-dependent macrophage uptake of apoptotic cells: Binding to lipoprotein receptor-related protein receptor family members

Sourindra N. Maiti, Krishnakumar Balasubramanian, Johanna A. Ramoth, Alan J. Schroit

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The recognition and removal of apoptotic cells is critical to development, tissue homeostasis, and the resolution of inflammation. Many studies have shown that phagocytosis is regulated by signaling mechanisms that involve distinct ligand-receptor interactions that drive the engulfment of apoptotic cells. Studies from our laboratory have shown that the plasma protein β-2-glycoprotein 1 (β2GP1), a member of the short consensus repeat superfamily, binds phosphatidylserine-containing vesicles and apoptotic cells and promotes their bridging and subsequent engulfment by phagocytes. The phagocyte receptor for the protein/apoptotic cell complex, however, is unknown. Here we report that a member of the low density lipoprotein receptor-related protein family on phagocytes binds and facilitates engulfment of β2GP1-phosphatidylserine and β2GP1-apoptotic cell complexes. Using recombinant β2GP1, we also show that β2GP1-dependent uptake is mediated by bridging of the target cell to the phagocyte through the protein C- and N-terminal domains, respectively.

Original languageEnglish (US)
Pages (from-to)3761-3766
Number of pages6
JournalJournal of Biological Chemistry
Volume283
Issue number7
DOIs
StatePublished - Feb 15 2008

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Lipoprotein Receptors
Macrophages
Glycoproteins
Cells
Phagocytes
Phosphatidylserines
Proteins
Tissue homeostasis
LDL-Receptor Related Proteins
LDL Receptors
Protein C
Blood Proteins
Phagocytosis
Consensus
Ligands
Homeostasis
Inflammation

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

β-2-glycoprotein 1-dependent macrophage uptake of apoptotic cells : Binding to lipoprotein receptor-related protein receptor family members. / Maiti, Sourindra N.; Balasubramanian, Krishnakumar; Ramoth, Johanna A.; Schroit, Alan J.

In: Journal of Biological Chemistry, Vol. 283, No. 7, 15.02.2008, p. 3761-3766.

Research output: Contribution to journalArticle

Maiti, Sourindra N. ; Balasubramanian, Krishnakumar ; Ramoth, Johanna A. ; Schroit, Alan J. / β-2-glycoprotein 1-dependent macrophage uptake of apoptotic cells : Binding to lipoprotein receptor-related protein receptor family members. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 7. pp. 3761-3766.
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