β-adrenergic receptor activation promotes process outgrowth in an embryonic rat basal forebrain cell line and in primary neurons

John H. Kwon, Eva M. Eves, Stephen Farrell, Jose Segovia, Allan J. Tobin, Bruce H. Wainer, Martha Downen

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A clonal cell line, AS583-8.E4.22, from the embryonic day 15 rat basal forebrain was established using retrovirus-mediated transduction of a temperature-sensitive mutant of the simian virus 40 (SV40) large tumour antigen. The cell line expresses cytoskeletal and neurotransmitter features indicative of neuronal commitment. In response to agents that increase intracellular cAMP, including forskolin and catecholamines, the cell line exhibits rapid process outgrowth and growth cone formation that does not require new gene expression or protein synthesis. The neurite outgrowth induced by catecholamines is mediated by β2-adrenergic receptors and is characterized by a rapid, reversible redistribution of filamentous actin. Neurons from primary cultures of embryonic day 15 basal forebrain were also found to respond to β-adrenergic receptor agonists by enhancing growth cone formation. These results suggest that catecholamines provide cues that induce cytoskeletal rearrangements leading to neuronal process outgrowth and growth cone formation in the developing basal forebrain and possibly other neuronal progenitor cell populations. The neuronal basal forebrain cell line provides an ideal model to study the signalling mechanisms underlying the catecholamine-induced process outgrowth.

Original languageEnglish (US)
Pages (from-to)2042-2055
Number of pages14
JournalEuropean Journal of Neuroscience
Volume8
Issue number10
DOIs
StatePublished - Oct 29 1996

Fingerprint

Growth Cones
Adrenergic Receptors
Catecholamines
Neurons
Cell Line
Adrenergic Agonists
Simian virus 40
Neoplasm Antigens
Colforsin
Retroviridae
Cues
Neurotransmitter Agents
Actins
Stem Cells
Gene Expression
Temperature
Basal Forebrain
Population
Proteins
Neuronal Outgrowth

Keywords

  • β-adrenergic receptor
  • Forskolin
  • Process outgrowth
  • Retroviral transduction

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

β-adrenergic receptor activation promotes process outgrowth in an embryonic rat basal forebrain cell line and in primary neurons. / Kwon, John H.; Eves, Eva M.; Farrell, Stephen; Segovia, Jose; Tobin, Allan J.; Wainer, Bruce H.; Downen, Martha.

In: European Journal of Neuroscience, Vol. 8, No. 10, 29.10.1996, p. 2042-2055.

Research output: Contribution to journalArticle

Kwon, John H. ; Eves, Eva M. ; Farrell, Stephen ; Segovia, Jose ; Tobin, Allan J. ; Wainer, Bruce H. ; Downen, Martha. / β-adrenergic receptor activation promotes process outgrowth in an embryonic rat basal forebrain cell line and in primary neurons. In: European Journal of Neuroscience. 1996 ; Vol. 8, No. 10. pp. 2042-2055.
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