11Beta‐Hydroxysteroid Dehydrogenase Messenger Ribonucleic Acid Expression, Bioactivity and Immunoreactivity in Rat Cerebellum

M. P. Moisan; J. R. Seckl; L. P. Brett; C. Monder; A. K. Agarwal; P. C. White; C. R W Edwards

doi:10.1111/j.1365-2826.1990.tb00651.x

11Beta‐Hydroxysteroid Dehydrogenase Messenger Ribonucleic Acid Expression, Bioactivity and Immunoreactivity in Rat Cerebellum

M. P. Moisan, J. R. Seckl, L. P. Brett, C. Monder, A. K. Agarwal, P. C. White, C. R W Edwards

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

11β‐Hydroxysteroid dehydrogenase (11β‐OHSD) metabolizes corticosterone to inactive 11‐dehydrocorticosterone and thus protects non‐specific mineralocorticoid receptors from exposure to corticosterone in the kidney in vivo. Clearly, 11β‐OHSD might also regulate corticosterone access to glucocorticoid receptors. We have investigated cerebellum, a tissue with high glucocorticoid receptor, but very low mineralocorticoid receptor levels and have shown marked 11β‐OHSD bioactivity with similar co‐substrate requirements and inhibition kinetics to the renal enzyme. 11β‐OHSD messenger ribonucleic acid was expressed in cerebellum and was localized in Purkinje and granule cells. This distribution was confirmed immunohistochemically. Thus, we provide evidence for 11β‐OHSD in cerebellum and suggest that it may regulate the access of corticosterone to glucocorticoid receptors in addition to mineralocorticoid receptors.

Original language	English (US)
Pages (from-to)	853-858
Number of pages	6
Journal	Journal of Neuroendocrinology
Volume	2
Issue number	6
DOIs	https://doi.org/10.1111/j.1365-2826.1990.tb00651.x
State	Published - Dec 1990

Keywords

11β‐hydroxysteroid dehydrogenase
cerebellum
corticosterone
in situ hybridization
steroid receptors

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology
Endocrine and Autonomic Systems
Cellular and Molecular Neuroscience

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10.1111/j.1365-2826.1990.tb00651.x

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title = "11Beta‐Hydroxysteroid Dehydrogenase Messenger Ribonucleic Acid Expression, Bioactivity and Immunoreactivity in Rat Cerebellum",

abstract = "11β‐Hydroxysteroid dehydrogenase (11β‐OHSD) metabolizes corticosterone to inactive 11‐dehydrocorticosterone and thus protects non‐specific mineralocorticoid receptors from exposure to corticosterone in the kidney in vivo. Clearly, 11β‐OHSD might also regulate corticosterone access to glucocorticoid receptors. We have investigated cerebellum, a tissue with high glucocorticoid receptor, but very low mineralocorticoid receptor levels and have shown marked 11β‐OHSD bioactivity with similar co‐substrate requirements and inhibition kinetics to the renal enzyme. 11β‐OHSD messenger ribonucleic acid was expressed in cerebellum and was localized in Purkinje and granule cells. This distribution was confirmed immunohistochemically. Thus, we provide evidence for 11β‐OHSD in cerebellum and suggest that it may regulate the access of corticosterone to glucocorticoid receptors in addition to mineralocorticoid receptors.",

keywords = "11β‐hydroxysteroid dehydrogenase, cerebellum, corticosterone, in situ hybridization, steroid receptors",

author = "Moisan, {M. P.} and Seckl, {J. R.} and Brett, {L. P.} and C. Monder and Agarwal, {A. K.} and White, {P. C.} and Edwards, {C. R W}",

year = "1990",

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doi = "10.1111/j.1365-2826.1990.tb00651.x",

language = "English (US)",

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AU - Moisan, M. P.

AU - Seckl, J. R.

AU - Brett, L. P.

AU - Monder, C.

AU - Agarwal, A. K.

AU - White, P. C.

AU - Edwards, C. R W

PY - 1990/12

Y1 - 1990/12

N2 - 11β‐Hydroxysteroid dehydrogenase (11β‐OHSD) metabolizes corticosterone to inactive 11‐dehydrocorticosterone and thus protects non‐specific mineralocorticoid receptors from exposure to corticosterone in the kidney in vivo. Clearly, 11β‐OHSD might also regulate corticosterone access to glucocorticoid receptors. We have investigated cerebellum, a tissue with high glucocorticoid receptor, but very low mineralocorticoid receptor levels and have shown marked 11β‐OHSD bioactivity with similar co‐substrate requirements and inhibition kinetics to the renal enzyme. 11β‐OHSD messenger ribonucleic acid was expressed in cerebellum and was localized in Purkinje and granule cells. This distribution was confirmed immunohistochemically. Thus, we provide evidence for 11β‐OHSD in cerebellum and suggest that it may regulate the access of corticosterone to glucocorticoid receptors in addition to mineralocorticoid receptors.

AB - 11β‐Hydroxysteroid dehydrogenase (11β‐OHSD) metabolizes corticosterone to inactive 11‐dehydrocorticosterone and thus protects non‐specific mineralocorticoid receptors from exposure to corticosterone in the kidney in vivo. Clearly, 11β‐OHSD might also regulate corticosterone access to glucocorticoid receptors. We have investigated cerebellum, a tissue with high glucocorticoid receptor, but very low mineralocorticoid receptor levels and have shown marked 11β‐OHSD bioactivity with similar co‐substrate requirements and inhibition kinetics to the renal enzyme. 11β‐OHSD messenger ribonucleic acid was expressed in cerebellum and was localized in Purkinje and granule cells. This distribution was confirmed immunohistochemically. Thus, we provide evidence for 11β‐OHSD in cerebellum and suggest that it may regulate the access of corticosterone to glucocorticoid receptors in addition to mineralocorticoid receptors.

KW - 11β‐hydroxysteroid dehydrogenase

KW - cerebellum

KW - corticosterone

KW - in situ hybridization

KW - steroid receptors

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11Beta‐Hydroxysteroid Dehydrogenase Messenger Ribonucleic Acid Expression, Bioactivity and Immunoreactivity in Rat Cerebellum

Abstract

Keywords

ASJC Scopus subject areas

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