14-3-3ε is required for germ cell migration in drosophila

K. Kirki Tsigkari, Summer F. Acevedo, Efthimios M C Skoulakis

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Although 14-3-3 proteins participate in multiple biological processes, isoform-specific specialized functions, as well as functional redundancy are emerging with tissue and developmental stage-specificity. Accordingly, the two 14-3-3ε proteins in Drosophila exhibit functional specificity and redundancy. Homozygotes for loss of function alleles of D14-3-3ε contain significantly fewer germ line cells (pole cells) in their gonads, a phenotype not shared by mutants in the other 14-3-3 gene leo. We show that although D14-3-3ε is enriched within pole cells it is required in mesodermal somatic gonad precursor cells which guide pole cells in their migration through the mesoderm and coalesce with them to form the embryonic gonad. Loss of D14-3-3ε results in defective pole cell migration, reduced pole cell number. We present evidence that D14-3-3ε loss results in reduction or loss of the transcription factor Zfh-1, one of the main regulatory molecules of the pole cell migration, from the somatic gonad precursor cells.

Original languageEnglish (US)
Article numbere36702
JournalPloS one
Volume7
Issue number5
DOIs
StatePublished - May 30 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Tsigkari, K. K., Acevedo, S. F., & Skoulakis, E. M. C. (2012). 14-3-3ε is required for germ cell migration in drosophila. PloS one, 7(5), [e36702]. https://doi.org/10.1371/journal.pone.0036702