14-3-3ε is required for germ cell migration in drosophila

K. Kirki Tsigkari, Summer F. Acevedo, Efthimios M C Skoulakis

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Although 14-3-3 proteins participate in multiple biological processes, isoform-specific specialized functions, as well as functional redundancy are emerging with tissue and developmental stage-specificity. Accordingly, the two 14-3-3ε proteins in Drosophila exhibit functional specificity and redundancy. Homozygotes for loss of function alleles of D14-3-3ε contain significantly fewer germ line cells (pole cells) in their gonads, a phenotype not shared by mutants in the other 14-3-3 gene leo. We show that although D14-3-3ε is enriched within pole cells it is required in mesodermal somatic gonad precursor cells which guide pole cells in their migration through the mesoderm and coalesce with them to form the embryonic gonad. Loss of D14-3-3ε results in defective pole cell migration, reduced pole cell number. We present evidence that D14-3-3ε loss results in reduction or loss of the transcription factor Zfh-1, one of the main regulatory molecules of the pole cell migration, from the somatic gonad precursor cells.

Original languageEnglish (US)
Article numbere36702
JournalPLoS One
Volume7
Issue number5
DOIs
StatePublished - May 30 2012

Fingerprint

cell movement
Germ Cells
Drosophila
Cell Movement
Gonads
Poles
germ cells
Cells
gonads
14-3-3 Proteins
cells
Redundancy
Biological Phenomena
Homozygote
Mesoderm
homozygosity
Protein Isoforms
Transcription Factors
Cell Count
Alleles

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Tsigkari, K. K., Acevedo, S. F., & Skoulakis, E. M. C. (2012). 14-3-3ε is required for germ cell migration in drosophila. PLoS One, 7(5), [e36702]. https://doi.org/10.1371/journal.pone.0036702

14-3-3ε is required for germ cell migration in drosophila. / Tsigkari, K. Kirki; Acevedo, Summer F.; Skoulakis, Efthimios M C.

In: PLoS One, Vol. 7, No. 5, e36702, 30.05.2012.

Research output: Contribution to journalArticle

Tsigkari, KK, Acevedo, SF & Skoulakis, EMC 2012, '14-3-3ε is required for germ cell migration in drosophila', PLoS One, vol. 7, no. 5, e36702. https://doi.org/10.1371/journal.pone.0036702
Tsigkari, K. Kirki ; Acevedo, Summer F. ; Skoulakis, Efthimios M C. / 14-3-3ε is required for germ cell migration in drosophila. In: PLoS One. 2012 ; Vol. 7, No. 5.
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