15-Hydroxyprostaglandin dehydrogenase, a COX-2 oncogene antagonist, is a TGF-β-induced suppressor of human gastrointestinal cancers

Min Yan, Ronald M. Rerko, Petra Platzer, Dawn Dawson, Joseph Willis, Min Tong, Earl Lawrence, James Lutterbaugh, Shilong Lu, James K V Willson, Guangbin Luo, Jack Hensold, Hsin Hsiung Tai, Keith Wilson, Sanford D. Markowitz

Research output: Contribution to journalArticle

187 Citations (Scopus)

Abstract

Marked increased expression of cyclooxygenase 2 (COX-2), a prostaglandin-synthesizing enzyme that is pharmacologically inhibited by nonsteroid anti-inflammatory-type drugs, is a major early oncogenic event in the genesis of human colon neoplasia. We report that, in addition to inducing expression of COX-2, colon cancers further target the prostaglandin biogenesis pathway by ubiquitously abrogating expression of 15-hydroxyprostaglandin dehydrogenase (15-PGDH), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2. We find that 15-PGDH transcript and protein are both highly expressed by normal colonic epithelia but are nearly undetectable in colon cancers. Using gene transfection to restore 15-PGDH expression in colon cancer cells strongly inhibits the ability of these cells to form tumors in immune-deficient mice and demonstrates 15-PGDH to have functional colon cancer tumor suppressor activity. In interrogating the mechanism for 15-PGDH expression loss in colon cancer, we determined that colonic 15-PGDH expression is directly controlled and strongly induced by activation of the TGF-β tumor suppressor pathway. These findings thus delineate an enzymatic pathway that induces colon cancer suppression, a pathway that is activated by TGF-β and mediated by 15-PGDH.

Original languageEnglish (US)
Pages (from-to)17468-17473
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number50
DOIs
StatePublished - Dec 14 2004

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15-hydroxyprostaglandin dehydrogenase
Gastrointestinal Neoplasms
Cyclooxygenase 2
Oncogenes
Colonic Neoplasms
Prostaglandins
Neoplasms
Enzymes
Transfection
Colon
Anti-Inflammatory Agents
Epithelium

Keywords

  • Colon
  • Gastric

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

15-Hydroxyprostaglandin dehydrogenase, a COX-2 oncogene antagonist, is a TGF-β-induced suppressor of human gastrointestinal cancers. / Yan, Min; Rerko, Ronald M.; Platzer, Petra; Dawson, Dawn; Willis, Joseph; Tong, Min; Lawrence, Earl; Lutterbaugh, James; Lu, Shilong; Willson, James K V; Luo, Guangbin; Hensold, Jack; Tai, Hsin Hsiung; Wilson, Keith; Markowitz, Sanford D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 50, 14.12.2004, p. 17468-17473.

Research output: Contribution to journalArticle

Yan, M, Rerko, RM, Platzer, P, Dawson, D, Willis, J, Tong, M, Lawrence, E, Lutterbaugh, J, Lu, S, Willson, JKV, Luo, G, Hensold, J, Tai, HH, Wilson, K & Markowitz, SD 2004, '15-Hydroxyprostaglandin dehydrogenase, a COX-2 oncogene antagonist, is a TGF-β-induced suppressor of human gastrointestinal cancers', Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 50, pp. 17468-17473. https://doi.org/10.1073/pnas.0406142101
Yan, Min ; Rerko, Ronald M. ; Platzer, Petra ; Dawson, Dawn ; Willis, Joseph ; Tong, Min ; Lawrence, Earl ; Lutterbaugh, James ; Lu, Shilong ; Willson, James K V ; Luo, Guangbin ; Hensold, Jack ; Tai, Hsin Hsiung ; Wilson, Keith ; Markowitz, Sanford D. / 15-Hydroxyprostaglandin dehydrogenase, a COX-2 oncogene antagonist, is a TGF-β-induced suppressor of human gastrointestinal cancers. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 50. pp. 17468-17473.
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