This chapter discusses the two ways of constructing antibody-toxin conjugates with cell-type specificity. The first is to link the antibody by a disulfide bond to the isolated A chain moiety or alternatively to one of the single-chain plant peptides, such as gelonin from Gelonium multiflorum, whose damaging action on eukaryotic ribosomes is apparently identical to that of the A chains of abrin and ricin. The second is to link the intact toxin to the antibody and block the cell recognition site on the B chain to prevent the conjugate from binding to and killing cells nonspecifically. This chapter describes the methods for preparing these two types of conjugate and characterizing them physicochemically and biologically. The chapter presents the advantages and limitations of a chain conjugates to those of intact toxin conjugates as selective cytotoxic agents in vitro and as chemotherapeutic agents in animals.
ASJC Scopus subject areas
- Molecular Biology