17β-Hydroxysteroid oxidoreductases of human fetal and adult tissues: Immunological cross-reactivity with an anti-human placental cytosolic 17β-hydroxysteroid oxidoreductase antibody

L. Milewich, B. R. Carr, R. A. Frenkel, M. Marrero, P. C. Macdonald, J. I. Mason

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14 Scopus citations

Abstract

To determine whether the immunological determinants of human placental 17β-hydroxysteroid oxidoreductase (17β-HSOR) were present in 17β-HSORs of tissues of the human fetus and adult and of various non-human cells maintained in culture, western immunoblot analysis was conducted by use of a polyclonal antibody directed against determinants of the placental cytosolic enzyme. Tissues and cells were evaluated for the presence of immunocross-reactive proteins with a relative molecular mass (Mr) similar to that of placental 17β-HSOR (∼ 34 000). By use of homogenates of human fetal tissues, immunostaining of 17β-HSORs of Mr∼34 kDa was detected in trophoblast, fetal adrenal neocortex, fetal zone of the adrenal gland, liver, intestine, kidney, brain, lung, skin, heart, spleen, pancreas, chorion laeve, and, occasionally, amnion. Immunostaining at Mr∼34 kDa also was demonstrated by use of cytosolic preparations of fetal tissues and, in some cases, by use of unwashed microsomal fractions; this protein was either absent or present in almost undetectable amounts in washed microsomes, except for placenta and fetal brain. Immunostaining at ∼34 kDa was demonstrated occasionally in decidua of pregnant women by use of homogenates, but was not detected in endometrium or myometrium of non-pregnant women, testis of an adult man, mouse and rat Leydig tumour cells, mouse and rat adrenal tumour cells, and normal bovine adrenocortical cells.

Original languageEnglish (US)
Pages (from-to)95-108
Number of pages14
JournalPlacenta
Volume11
Issue number2
DOIs
StatePublished - Jan 1 1990

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ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Developmental Biology

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