TY - JOUR
T1 - [2] Functional groups on enzymes suitable for binding to matrices
AU - Srere, Paul A.
AU - Uyeda, Kosaku
N1 - Funding Information:
Celluloses have been activated by use of cyanuric chloride and some of its diehloro derivatives. Reaction then can occur with amino groups in proteins. In addition, supports containing isothiocyanates have been used for the covalent coupling of proteins2 Acknowledgment This work was supported in part by Grant No. GB41851 from the National Science Foundation.
PY - 1976/1/1
Y1 - 1976/1/1
N2 - Proteins can be immobilized (active but not soluble) in a number of different ways. When a protein is covalently coupled to an insoluble support, two techniques may be employed. One technique is to activate the support material for reaction with groups on the protein. Second, one can use a coupling reagent to link protein to matrix. A third possibility would be to activate the protein molecule for coupling to the support material. This chapter discusses the groups on the protein that is available for covalent coupling to various matrices and some of the most commonly used procedures. The reactivity of a certain group in the protein depends on the location and environment in which the amino acid residues are placed. Because the proteins are large molecules, many of the site changes are partially “buried” and may be relatively inert, while some residues that are exposed to the solvent may be highly reactive. The principles discussed in the chapter concerning the reaction of the amino acid residues on protein molecules with an active group on some support material, are also applicable to the other methods involving covalent coupling of the protein.
AB - Proteins can be immobilized (active but not soluble) in a number of different ways. When a protein is covalently coupled to an insoluble support, two techniques may be employed. One technique is to activate the support material for reaction with groups on the protein. Second, one can use a coupling reagent to link protein to matrix. A third possibility would be to activate the protein molecule for coupling to the support material. This chapter discusses the groups on the protein that is available for covalent coupling to various matrices and some of the most commonly used procedures. The reactivity of a certain group in the protein depends on the location and environment in which the amino acid residues are placed. Because the proteins are large molecules, many of the site changes are partially “buried” and may be relatively inert, while some residues that are exposed to the solvent may be highly reactive. The principles discussed in the chapter concerning the reaction of the amino acid residues on protein molecules with an active group on some support material, are also applicable to the other methods involving covalent coupling of the protein.
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U2 - 10.1016/S0076-6879(76)44004-1
DO - 10.1016/S0076-6879(76)44004-1
M3 - Article
C2 - 1021677
AN - SCOPUS:0017248834
SN - 0076-6879
VL - 44
SP - 11
EP - 19
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -