2-methoxyestradiol prevents LNCaP tumor development in nude mice: Potential role of G 2/M regulatory proteins

Cara Horny, Muralimanoharan Sri Balasubashini, Krishna Komanduri, Manonmani Ganapathy, I. Tien Yeh, Rita Ghosh, Addanki P. Kumar

Research output: Contribution to journalArticle

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Abstract

Nontoxic naturally occurring metabolite of estrogen namely 2-methoxyestradial (2ME 2) found in serum and urine has been shown to be antitumorigenic in various tumor models including the prostate. A recent study conducted in breast cancer cells showed growth stimulatory effect of 2ME 2 when used at low concentrations (10-750 nM). Studies from our laboratory has demonstrated prostate tumor preventive ability of 50 mg/kg 2-ME 2. In this study we show that concentrations of 2-ME 2 as low as 1 μM is sufficient to inhibit proliferation and induce apoptosis in androgen responsive LNCaP cells. In addition oral administration of doses lower than 50 mg/kg prevented prostate tumor development in LNCaP xenograft model. The observed tumor growth inhibition was associated with induction of apoptosis, increased expression of Wee1 kinase and p34cdc2. In addition administration of 25 mg/kg 2-ME 2 prevented tumor development significantly that is associated with reduction in serum PSA levels.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalJournal of Cell Death
Volume2009
StatePublished - Dec 1 2009

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Nude Mice
Tumors
Mercaptoethanol
Prostate
Neoplasms
Proteins
Apoptosis
Cell growth
Growth
Metabolites
Serum
Heterografts
Androgens
Oral Administration
Estrogens
Phosphotransferases
2-methoxyestradiol
Urine
Breast Neoplasms

Keywords

  • 2-methoxyestradiol
  • Apoptosis
  • Prostate cancer prevention
  • Psa

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Horny, C., Sri Balasubashini, M., Komanduri, K., Ganapathy, M., Yeh, I. T., Ghosh, R., & Kumar, A. P. (2009). 2-methoxyestradiol prevents LNCaP tumor development in nude mice: Potential role of G 2/M regulatory proteins. Journal of Cell Death, 2009, 1-8.

2-methoxyestradiol prevents LNCaP tumor development in nude mice : Potential role of G 2/M regulatory proteins. / Horny, Cara; Sri Balasubashini, Muralimanoharan; Komanduri, Krishna; Ganapathy, Manonmani; Yeh, I. Tien; Ghosh, Rita; Kumar, Addanki P.

In: Journal of Cell Death, Vol. 2009, 01.12.2009, p. 1-8.

Research output: Contribution to journalArticle

Horny, C, Sri Balasubashini, M, Komanduri, K, Ganapathy, M, Yeh, IT, Ghosh, R & Kumar, AP 2009, '2-methoxyestradiol prevents LNCaP tumor development in nude mice: Potential role of G 2/M regulatory proteins', Journal of Cell Death, vol. 2009, pp. 1-8.
Horny C, Sri Balasubashini M, Komanduri K, Ganapathy M, Yeh IT, Ghosh R et al. 2-methoxyestradiol prevents LNCaP tumor development in nude mice: Potential role of G 2/M regulatory proteins. Journal of Cell Death. 2009 Dec 1;2009:1-8.
Horny, Cara ; Sri Balasubashini, Muralimanoharan ; Komanduri, Krishna ; Ganapathy, Manonmani ; Yeh, I. Tien ; Ghosh, Rita ; Kumar, Addanki P. / 2-methoxyestradiol prevents LNCaP tumor development in nude mice : Potential role of G 2/M regulatory proteins. In: Journal of Cell Death. 2009 ; Vol. 2009. pp. 1-8.
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