20-Hydroxyeicosatetraenoic acid is excreted as a glucuronide conjugate in human urine

Chandra Prakash, Ji Y. Zhang, J R Falck, Kamlesh Chauhan, Ian A. Blair

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

20-hydroxyeicosatetraenoic acid, a major renal P-450 metabolite of arachidonic acid, has been quantified in human urine using capillary gas chromatography/electron capture negative ion chemical ionization mass spectrometry. The urinary excretion of 20-hydroxyeicosatetraenoic acid was in the low pg/ml range. However, treatment of urine with β-glucuronidase resulted in a 13- to 28-fold increase in its concentration. This suggests 20-hydroxyeicosatetraenoic acid differs from other eicosanoids in that it is excreted primarily as a glucuronide conjugate.

Original languageEnglish (US)
Pages (from-to)728-733
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume185
Issue number2
DOIs
StatePublished - Jun 15 1992

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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