20-Hydroxyeicosatetraenoic acid is excreted as a glucuronide conjugate in human urine

Chandra Prakash; Ji Y. Zhang; J R Falck; Kamlesh Chauhan; Ian A. Blair

doi:10.1016/0006-291X(92)91686-K

20-Hydroxyeicosatetraenoic acid is excreted as a glucuronide conjugate in human urine

Chandra Prakash, Ji Y. Zhang, J R Falck, Kamlesh Chauhan, Ian A. Blair

Biochemistry

Research output: Contribution to journal › Article › peer-review

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Abstract

20-hydroxyeicosatetraenoic acid, a major renal P-450 metabolite of arachidonic acid, has been quantified in human urine using capillary gas chromatography/electron capture negative ion chemical ionization mass spectrometry. The urinary excretion of 20-hydroxyeicosatetraenoic acid was in the low pg/ml range. However, treatment of urine with β-glucuronidase resulted in a 13- to 28-fold increase in its concentration. This suggests 20-hydroxyeicosatetraenoic acid differs from other eicosanoids in that it is excreted primarily as a glucuronide conjugate.

Original language	English (US)
Pages (from-to)	728-733
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	185
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(92)91686-K
State	Published - Jun 15 1992

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "20-hydroxyeicosatetraenoic acid, a major renal P-450 metabolite of arachidonic acid, has been quantified in human urine using capillary gas chromatography/electron capture negative ion chemical ionization mass spectrometry. The urinary excretion of 20-hydroxyeicosatetraenoic acid was in the low pg/ml range. However, treatment of urine with β-glucuronidase resulted in a 13- to 28-fold increase in its concentration. This suggests 20-hydroxyeicosatetraenoic acid differs from other eicosanoids in that it is excreted primarily as a glucuronide conjugate.",

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AU - Prakash, Chandra

AU - Zhang, Ji Y.

AU - Falck, J R

AU - Chauhan, Kamlesh

AU - Blair, Ian A.

PY - 1992/6/15

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N2 - 20-hydroxyeicosatetraenoic acid, a major renal P-450 metabolite of arachidonic acid, has been quantified in human urine using capillary gas chromatography/electron capture negative ion chemical ionization mass spectrometry. The urinary excretion of 20-hydroxyeicosatetraenoic acid was in the low pg/ml range. However, treatment of urine with β-glucuronidase resulted in a 13- to 28-fold increase in its concentration. This suggests 20-hydroxyeicosatetraenoic acid differs from other eicosanoids in that it is excreted primarily as a glucuronide conjugate.

AB - 20-hydroxyeicosatetraenoic acid, a major renal P-450 metabolite of arachidonic acid, has been quantified in human urine using capillary gas chromatography/electron capture negative ion chemical ionization mass spectrometry. The urinary excretion of 20-hydroxyeicosatetraenoic acid was in the low pg/ml range. However, treatment of urine with β-glucuronidase resulted in a 13- to 28-fold increase in its concentration. This suggests 20-hydroxyeicosatetraenoic acid differs from other eicosanoids in that it is excreted primarily as a glucuronide conjugate.

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20-Hydroxyeicosatetraenoic acid is excreted as a glucuronide conjugate in human urine

Abstract

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