2B4+ CD8+ T cells play an inhibitory role against constrained HIV epitopes

Kim N. Aldy; Nathan C. Horton; Porunelloor A. Mathew; Stephen O. Mathew

doi:10.1016/j.bbrc.2011.01.062

2B4⁺ CD8⁺ T cells play an inhibitory role against constrained HIV epitopes

Kim N. Aldy, Nathan C. Horton, Porunelloor A. Mathew, Stephen O. Mathew

Emergency Medicine

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Cytotoxic T cells play a critical role in the control of HIV and the progression of infected individuals to AIDS. 2B4 (CD244) is a member of the SLAM family of receptors that regulate lymphocyte development and function. The expression of 2B4 on CD8⁺ T cells was shown to increase during AIDS disease progression. However, the functional role of 2B4⁺ CD8⁺ T cells against HIV infection is not known. Here, we have examined the functional role of 2B4⁺ CD8⁺ T cells during and after stimulation with HLA B14 or B27 restricted HIV epitopes. Interestingly, IFN-γ secretion and cytotoxic activity of 2B4⁺ CD8⁺ T cells stimulated with HIV peptides were significantly decreased when compared to influenza peptide stimulated 2B4⁺ CD8⁺ T cells. The expression of the signaling adaptor molecule SAP was downregulated in 2B4⁺ CD8⁺ T cells upon HIV peptide stimulation. These results suggest that 2B4⁺ CD8⁺ T cells play an inhibitory role against constrained HIV epitopes underlying the inability to control the virus during disease progression.

Original language	English (US)
Pages (from-to)	503-507
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	405
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2011.01.062
State	Published - Feb 18 2011

Keywords

2B4
B lymphoblastoid cell line (BLCL)
Cell surface receptor
Cytotoxic T lymphocyte (CTL)
HIV epitopes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2011.01.062

Cite this

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title = "2B4+ CD8+ T cells play an inhibitory role against constrained HIV epitopes",

abstract = "Cytotoxic T cells play a critical role in the control of HIV and the progression of infected individuals to AIDS. 2B4 (CD244) is a member of the SLAM family of receptors that regulate lymphocyte development and function. The expression of 2B4 on CD8+ T cells was shown to increase during AIDS disease progression. However, the functional role of 2B4+ CD8+ T cells against HIV infection is not known. Here, we have examined the functional role of 2B4+ CD8+ T cells during and after stimulation with HLA B14 or B27 restricted HIV epitopes. Interestingly, IFN-γ secretion and cytotoxic activity of 2B4+ CD8+ T cells stimulated with HIV peptides were significantly decreased when compared to influenza peptide stimulated 2B4+ CD8+ T cells. The expression of the signaling adaptor molecule SAP was downregulated in 2B4+ CD8+ T cells upon HIV peptide stimulation. These results suggest that 2B4+ CD8+ T cells play an inhibitory role against constrained HIV epitopes underlying the inability to control the virus during disease progression.",

keywords = "2B4, B lymphoblastoid cell line (BLCL), Cell surface receptor, Cytotoxic T lymphocyte (CTL), HIV epitopes",

author = "Aldy, {Kim N.} and Horton, {Nathan C.} and Mathew, {Porunelloor A.} and Mathew, {Stephen O.}",

note = "Funding Information: This study was supported by the EXPORT center pilot grant # P20MD001633 from the National Center for Minority Health and Health Disparities (NCMHD) and a grant from Miller Brewing Company , Fort Worth, TX. We thank Dr. Xiangle Sun for assistance with flow cytometry. Flow cytometry was performed in the Flow Cytometry and Laser Capture Microdissection Core Facility at the University of North Texas Health Science Center. Flow Cytometry facility is supported by National Institutes of Health award ISIORR018999-01A1.",

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AU - Mathew, Stephen O.

N1 - Funding Information: This study was supported by the EXPORT center pilot grant # P20MD001633 from the National Center for Minority Health and Health Disparities (NCMHD) and a grant from Miller Brewing Company , Fort Worth, TX. We thank Dr. Xiangle Sun for assistance with flow cytometry. Flow cytometry was performed in the Flow Cytometry and Laser Capture Microdissection Core Facility at the University of North Texas Health Science Center. Flow Cytometry facility is supported by National Institutes of Health award ISIORR018999-01A1.

PY - 2011/2/18

Y1 - 2011/2/18

N2 - Cytotoxic T cells play a critical role in the control of HIV and the progression of infected individuals to AIDS. 2B4 (CD244) is a member of the SLAM family of receptors that regulate lymphocyte development and function. The expression of 2B4 on CD8+ T cells was shown to increase during AIDS disease progression. However, the functional role of 2B4+ CD8+ T cells against HIV infection is not known. Here, we have examined the functional role of 2B4+ CD8+ T cells during and after stimulation with HLA B14 or B27 restricted HIV epitopes. Interestingly, IFN-γ secretion and cytotoxic activity of 2B4+ CD8+ T cells stimulated with HIV peptides were significantly decreased when compared to influenza peptide stimulated 2B4+ CD8+ T cells. The expression of the signaling adaptor molecule SAP was downregulated in 2B4+ CD8+ T cells upon HIV peptide stimulation. These results suggest that 2B4+ CD8+ T cells play an inhibitory role against constrained HIV epitopes underlying the inability to control the virus during disease progression.

AB - Cytotoxic T cells play a critical role in the control of HIV and the progression of infected individuals to AIDS. 2B4 (CD244) is a member of the SLAM family of receptors that regulate lymphocyte development and function. The expression of 2B4 on CD8+ T cells was shown to increase during AIDS disease progression. However, the functional role of 2B4+ CD8+ T cells against HIV infection is not known. Here, we have examined the functional role of 2B4+ CD8+ T cells during and after stimulation with HLA B14 or B27 restricted HIV epitopes. Interestingly, IFN-γ secretion and cytotoxic activity of 2B4+ CD8+ T cells stimulated with HIV peptides were significantly decreased when compared to influenza peptide stimulated 2B4+ CD8+ T cells. The expression of the signaling adaptor molecule SAP was downregulated in 2B4+ CD8+ T cells upon HIV peptide stimulation. These results suggest that 2B4+ CD8+ T cells play an inhibitory role against constrained HIV epitopes underlying the inability to control the virus during disease progression.

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