3 Hydroxy 3 methylglutaryl coenzyme A reductase. Solubilization and purification of a cold sensitive microsomal enzyme

M. S. Brown, S. E. Dana, J. M. Dietschy, M. D. Siperstein

Research output: Contribution to journalArticle

73 Scopus citations

Abstract

3 Hydroxy 3 methyglutaryl coenzyme A reductase, the rate controlling enzyme of cholesterol biosynthesis in rat liver, was solubilized and purified 130 fold from rat liver microsomes. Evidence is presented to suggest that the enzyme is localized to the surface of the microsomal membrane. 3 Hydroxy 3 methylglutaryl CoA reductase can be removed from microsomes by mild treatments such as exposure to high salt or glycerol concentrations which leave the bi laminar structure of the microsomal membrane intact. The solubilized enzyme is rapidly inactivated by chilling to 4° and is protected from this cold induced inactivation by salt only at concentrations in the range of 4 M. 3 Hydroxy 3 methylglutaryl CoA reductase is the first solubilized microsomal enzyme reported to be cold sensitive.

Original languageEnglish (US)
Pages (from-to)4731-4738
Number of pages8
JournalJournal of Biological Chemistry
Volume248
Issue number13
StatePublished - Dec 1 1973

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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