3-Hydroxy-fatty acid analysis by gas chromatography-mass spectrometry.

Patricia M. Jones, Michael J. Bennett

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The mitochondrial fatty acid beta-oxidation is integral to normal cellular metabolism and maintenance of cellular energy supplies. Disorders of this pathway interrupt the body's ability to deal with fasting states, as well as compromising the functioning of organs and systems whose high-energy requirements utilize fats for a continuous energy source, such as heart and skeletal muscle. This method quantitatively measures intermediate metabolites of fatty acid beta-oxidation, specifically the 3-hydroxy-fatty acids produced by the third step in the pathway. The method is useful for helping to diagnose disorders of the pathway, especially defects in the L-3-hydroxyacyl CoA dehydrogenases. Serum or plasma samples are used for routine clinical evaluation; however, measurement of 3-hydroxy-fatty acid intermediates in fibroblast cell culture media and in samples from mice also allows the method to be used for research into fatty acid oxidation and interconnected pathways. The method is a stable isotope dilution, electron impact ionization gas chromatography/mass spectrometry (GC/MS) procedure.

Original languageEnglish (US)
Pages (from-to)229-243
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume603
DOIs
StatePublished - 2010

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Hydroxy Acids
Gas Chromatography-Mass Spectrometry
Fatty Acids
3-Hydroxyacyl CoA Dehydrogenases
Aptitude
Isotopes
Culture Media
Fasting
Myocardium
Skeletal Muscle
Cell Culture Techniques
Fibroblasts
Fats
Maintenance
Electrons
Serum
Research

ASJC Scopus subject areas

  • Medicine(all)

Cite this

3-Hydroxy-fatty acid analysis by gas chromatography-mass spectrometry. / Jones, Patricia M.; Bennett, Michael J.

In: Methods in molecular biology (Clifton, N.J.), Vol. 603, 2010, p. 229-243.

Research output: Contribution to journalArticle

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