39-kDa protein modulates binding of ligands to low density lipoprotein receptor-related protein/α2-macroglobulin receptor

Joachim Herz, Joseph L. Goldstein, Dudley K. Strickland, Y. K. Ho, Michael S. Brown

Research output: Contribution to journalArticlepeer-review

454 Scopus citations

Abstract

A 39-kDa protein of unknown function has previously been reported to copurify with the low density lipoprotein receptor-related protein (LRP)/α2-macroglobulin receptor. In this study we demonstrate that a recombinant 39-kDa fusion protein can reversibly bind to the 515-kDa subunit of the LRP/α2-macroglobulin receptor. This interaction inhibits the binding and uptake of the receptor's two known ligands: 1) β-migrating very low density lipoproteins activated by enrichment with apoprotein E and 2) α2-macroglobulin activated by incubation with plasma proteases or methylamine. A potential in vivo role of the 39-kDa protein is to modulate the uptake of apoE-enriched lipoproteins and activated α2-macroglobulin in hepatic and extrahepatic tissues.

Original languageEnglish (US)
Pages (from-to)21232-21238
Number of pages7
JournalJournal of Biological Chemistry
Volume266
Issue number31
StatePublished - 1991

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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