[40] Reduced Nicotinamide Adenine Dinucleotide Phosphate: Δ4-3-Ketosteroid 5α-Oxidoreductase (Rat Ventral Prostate)

Ronald J. Moore; Jean D. Wilson

doi:10.1016/S0076-6879(75)36043-6

[40] Reduced Nicotinamide Adenine Dinucleotide Phosphate: Δ⁴-3-Ketosteroid 5α-Oxidoreductase (Rat Ventral Prostate)

Ronald J. Moore, Jean D. Wilson

Internal Medicine

Research output: Contribution to journal › Article

38 Scopus citations

Abstract

The 5α-reduction of radioactive Δ⁴-3-ketosteroids, which do not contain substitutions on carbon-11 of the steroid molecule, is followed in the presence of an excess of NADPH by measuring the appearance of the radioactive 5α-reduced metabolites, utilizing a rapid thin-layer chromatographic separation. The assay in this chapter has been developed because the low activity of the enzyme in most tissues precludes the measurement of NADPH disappearance spectrophotometrically. The enzyme exhibits distinctive tissue localization in that most activity is found in the organs of accessory reproduction and in liver.

Original language	English (US)
Pages (from-to)	466-474
Number of pages	9
Journal	Methods in Enzymology
Volume	36
Issue number	C
DOIs	https://doi.org/10.1016/S0076-6879(75)36043-6
State	Published - Jan 1 1975

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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Moore, R. J., & Wilson, J. D. (1975). [40] Reduced Nicotinamide Adenine Dinucleotide Phosphate: Δ⁴-3-Ketosteroid 5α-Oxidoreductase (Rat Ventral Prostate). Methods in Enzymology, 36(C), 466-474. https://doi.org/10.1016/S0076-6879(75)36043-6

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