[40] Reduced Nicotinamide Adenine Dinucleotide Phosphate: Δ4-3-Ketosteroid 5α-Oxidoreductase (Rat Ventral Prostate)

Ronald J. Moore, Jean D. Wilson

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

The 5α-reduction of radioactive Δ4-3-ketosteroids, which do not contain substitutions on carbon-11 of the steroid molecule, is followed in the presence of an excess of NADPH by measuring the appearance of the radioactive 5α-reduced metabolites, utilizing a rapid thin-layer chromatographic separation. The assay in this chapter has been developed because the low activity of the enzyme in most tissues precludes the measurement of NADPH disappearance spectrophotometrically. The enzyme exhibits distinctive tissue localization in that most activity is found in the organs of accessory reproduction and in liver.

Original languageEnglish (US)
Pages (from-to)466-474
Number of pages9
JournalMethods in Enzymology
Volume36
Issue numberC
DOIs
StatePublished - Jan 1 1975

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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