TY - JOUR
T1 - [40] Reduced Nicotinamide Adenine Dinucleotide Phosphate
T2 - Δ4-3-Ketosteroid 5α-Oxidoreductase (Rat Ventral Prostate)
AU - Moore, Ronald J.
AU - Wilson, Jean D.
PY - 1975/1/1
Y1 - 1975/1/1
N2 - The 5α-reduction of radioactive Δ4-3-ketosteroids, which do not contain substitutions on carbon-11 of the steroid molecule, is followed in the presence of an excess of NADPH by measuring the appearance of the radioactive 5α-reduced metabolites, utilizing a rapid thin-layer chromatographic separation. The assay in this chapter has been developed because the low activity of the enzyme in most tissues precludes the measurement of NADPH disappearance spectrophotometrically. The enzyme exhibits distinctive tissue localization in that most activity is found in the organs of accessory reproduction and in liver.
AB - The 5α-reduction of radioactive Δ4-3-ketosteroids, which do not contain substitutions on carbon-11 of the steroid molecule, is followed in the presence of an excess of NADPH by measuring the appearance of the radioactive 5α-reduced metabolites, utilizing a rapid thin-layer chromatographic separation. The assay in this chapter has been developed because the low activity of the enzyme in most tissues precludes the measurement of NADPH disappearance spectrophotometrically. The enzyme exhibits distinctive tissue localization in that most activity is found in the organs of accessory reproduction and in liver.
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U2 - 10.1016/S0076-6879(75)36043-6
DO - 10.1016/S0076-6879(75)36043-6
M3 - Article
C2 - 234171
AN - SCOPUS:0016458753
VL - 36
SP - 466
EP - 474
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
IS - C
ER -