5α-Androstane-3α,17β-diol activates pathway that resembles the epidermal growth factor responsive pathways in stimulating human prostate cancer LNCaP cell proliferation

R. A. Zimmerman; I. Dozmorov; E. H. Nunlist; Y. Tang; X. Li; R. Cowan; M. Centola; M. B. Frank; D. J. Culkin; H. K. Lin

doi:10.1038/sj.pcan.4500761

5α-Androstane-3α,17β-diol activates pathway that resembles the epidermal growth factor responsive pathways in stimulating human prostate cancer LNCaP cell proliferation

R. A. Zimmerman, I. Dozmorov, E. H. Nunlist, Y. Tang, X. Li, R. Cowan, M. Centola, M. B. Frank, D. J. Culkin, H. K. Lin

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

5α-Androstane-3α,17β-diol (3α-diol) is considered to have no androgenic effects in androgen target organs unless it is oxidized to 5α-dihydrotestosterone (5α-DHT). We used microarray and bioinformatics to identify and compare 3α-diol and 5α-DHT responsive gene in human prostate LNCaP cells. Through a procedure called 'hypervariable determination', a similar set of 30 responsive genes involving signal transduction, transcription regulation, and cell proliferation were selected in 5α-DHT-, 3α-diol-, and epidermal growth factor (EGF)-treated samples. F-means cluster and networking procedures showed that the responsive pattern of these genes was more closely related between 3α-diol and EGF than between 5α-DHT and 3α-diol treatments. We conclude that 3α-diol is capable of stimulating prostate cell proliferation by eliciting EGF-like pathway in conjunction with androgen receptor pathway.

Original language	English (US)
Pages (from-to)	364-374
Number of pages	11
Journal	Prostate Cancer and Prostatic Diseases
Volume	7
Issue number	4
DOIs	https://doi.org/10.1038/sj.pcan.4500761
State	Published - Dec 2004

Keywords

17β-diol
5α-dihydrotestosterone
5αandrostane-3α
Androgen receptor
Epidermal growth factor
Microarray analysis

ASJC Scopus subject areas

Oncology
Urology
Cancer Research

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10.1038/sj.pcan.4500761

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Zimmerman, R. A., Dozmorov, I., Nunlist, E. H., Tang, Y., Li, X., Cowan, R., Centola, M., Frank, M. B., Culkin, D. J., & Lin, H. K. (2004). 5α-Androstane-3α,17β-diol activates pathway that resembles the epidermal growth factor responsive pathways in stimulating human prostate cancer LNCaP cell proliferation. Prostate Cancer and Prostatic Diseases, 7(4), 364-374. https://doi.org/10.1038/sj.pcan.4500761

5α-Androstane-3α,17β-diol activates pathway that resembles the epidermal growth factor responsive pathways in stimulating human prostate cancer LNCaP cell proliferation. / Zimmerman, R. A.; Dozmorov, I.; Nunlist, E. H. et al.
In: Prostate Cancer and Prostatic Diseases, Vol. 7, No. 4, 12.2004, p. 364-374.

Research output: Contribution to journal › Article › peer-review

Zimmerman, RA, Dozmorov, I, Nunlist, EH, Tang, Y, Li, X, Cowan, R, Centola, M, Frank, MB, Culkin, DJ & Lin, HK 2004, '5α-Androstane-3α,17β-diol activates pathway that resembles the epidermal growth factor responsive pathways in stimulating human prostate cancer LNCaP cell proliferation', Prostate Cancer and Prostatic Diseases, vol. 7, no. 4, pp. 364-374. https://doi.org/10.1038/sj.pcan.4500761

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abstract = "5α-Androstane-3α,17β-diol (3α-diol) is considered to have no androgenic effects in androgen target organs unless it is oxidized to 5α-dihydrotestosterone (5α-DHT). We used microarray and bioinformatics to identify and compare 3α-diol and 5α-DHT responsive gene in human prostate LNCaP cells. Through a procedure called 'hypervariable determination', a similar set of 30 responsive genes involving signal transduction, transcription regulation, and cell proliferation were selected in 5α-DHT-, 3α-diol-, and epidermal growth factor (EGF)-treated samples. F-means cluster and networking procedures showed that the responsive pattern of these genes was more closely related between 3α-diol and EGF than between 5α-DHT and 3α-diol treatments. We conclude that 3α-diol is capable of stimulating prostate cell proliferation by eliciting EGF-like pathway in conjunction with androgen receptor pathway.",

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author = "Zimmerman, {R. A.} and I. Dozmorov and Nunlist, {E. H.} and Y. Tang and X. Li and R. Cowan and M. Centola and Frank, {M. B.} and Culkin, {D. J.} and Lin, {H. K.}",

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AU - Nunlist, E. H.

AU - Tang, Y.

AU - Li, X.

AU - Cowan, R.

AU - Centola, M.

AU - Frank, M. B.

AU - Culkin, D. J.

AU - Lin, H. K.

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5α-Androstane-3α,17β-diol activates pathway that resembles the epidermal growth factor responsive pathways in stimulating human prostate cancer LNCaP cell proliferation

Abstract

Keywords

ASJC Scopus subject areas

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