5′ Adenosine monophosphate prevents collagen degradation in culture but does not prevent corneal ulceration

Michael B. Berman, Harrison D Cavanagh, Janet Gage

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Alkali-burned rabbit corneas were treated topically with 5′ adenosine monophosphate (5′ AMP) in an attempt to prevent corneal ulceration. Drug treatment did not reduce the frequency or the severity of ulceration compared to treatment with an osmotically-matched saline control. Ulcerating corneas were cultured subsequently in control medium or in medium containing 5′ AMP. Corneas that had received saline in vivo produced high levels of collagenase activity and showed extensive explant degradation when cultured in control medium. When cultured in the presence of 5′ AMP, however, such corneas produced barely-detectable levels of collagenase, and explants were not apparently degraded. Corneas that had received 5′ AMP in vivo also produced high levels of collagenase activity and showed extensive explant degradation when cultured in control medium. When cultured in medium containing 5′ AMP, however, such corneas showed minimal degradation as evidenced by their appearance and by the very low hydroxyproline levels in the media. Significantly, media harvested from such cultures contained high levels of collagenase activity.

Original languageEnglish (US)
Pages (from-to)391-397
Number of pages7
JournalExperimental Eye Research
Volume24
Issue number4
DOIs
StatePublished - 1977

Fingerprint

Adenosine Monophosphate
Cornea
Collagen
Collagenases
Hydroxyproline
Alkalies
Rabbits
Pharmaceutical Preparations

Keywords

  • 5′ AMP
  • collagenase
  • cornea
  • ulceration

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

5′ Adenosine monophosphate prevents collagen degradation in culture but does not prevent corneal ulceration. / Berman, Michael B.; Cavanagh, Harrison D; Gage, Janet.

In: Experimental Eye Research, Vol. 24, No. 4, 1977, p. 391-397.

Research output: Contribution to journalArticle

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abstract = "Alkali-burned rabbit corneas were treated topically with 5′ adenosine monophosphate (5′ AMP) in an attempt to prevent corneal ulceration. Drug treatment did not reduce the frequency or the severity of ulceration compared to treatment with an osmotically-matched saline control. Ulcerating corneas were cultured subsequently in control medium or in medium containing 5′ AMP. Corneas that had received saline in vivo produced high levels of collagenase activity and showed extensive explant degradation when cultured in control medium. When cultured in the presence of 5′ AMP, however, such corneas produced barely-detectable levels of collagenase, and explants were not apparently degraded. Corneas that had received 5′ AMP in vivo also produced high levels of collagenase activity and showed extensive explant degradation when cultured in control medium. When cultured in medium containing 5′ AMP, however, such corneas showed minimal degradation as evidenced by their appearance and by the very low hydroxyproline levels in the media. Significantly, media harvested from such cultures contained high levels of collagenase activity.",
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N2 - Alkali-burned rabbit corneas were treated topically with 5′ adenosine monophosphate (5′ AMP) in an attempt to prevent corneal ulceration. Drug treatment did not reduce the frequency or the severity of ulceration compared to treatment with an osmotically-matched saline control. Ulcerating corneas were cultured subsequently in control medium or in medium containing 5′ AMP. Corneas that had received saline in vivo produced high levels of collagenase activity and showed extensive explant degradation when cultured in control medium. When cultured in the presence of 5′ AMP, however, such corneas produced barely-detectable levels of collagenase, and explants were not apparently degraded. Corneas that had received 5′ AMP in vivo also produced high levels of collagenase activity and showed extensive explant degradation when cultured in control medium. When cultured in medium containing 5′ AMP, however, such corneas showed minimal degradation as evidenced by their appearance and by the very low hydroxyproline levels in the media. Significantly, media harvested from such cultures contained high levels of collagenase activity.

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