5-Methylcytosine is localized in nucleosomes that contain histone H1

D. J. Ball, D. S. Gross, W. T. Garrard

Research output: Contribution to journalArticlepeer-review

80 Scopus citations


We have developed a procedure for purifying highly specific polyclonal antibodies against 5-methylcytosine. These antibodies were used to probe the distribution of 5-methylcytosine among fractionated nucleosomes of mouse cell chromatin. Our results demonstrate that at least 80% of the 5-methylcytosine is localized in nucleosomes that contain histone H1. Native nucleosomes that lack histone H1 or possess high mobility group proteins package DNA that is 1.6- to 2.3-fold undermethylated. We suggest that the preferential association of methylated sequences with histone H1 has functional significance because DNA methylation has been linked to gene inactivation and histone H1 is known to promote chromatin condensation.

Original languageEnglish (US)
Pages (from-to)5490-5494
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number181
StatePublished - 1983

ASJC Scopus subject areas

  • General


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