Abstract
We examined the mechanism by which the cytochrome P-450 metabolite of arachidonate, 5,6-epoxyeicosatrienoic acid (5,6-EET), modulates electrogenic transport in the rabbit cortical collecting duct (CCD). 5,6-EET depolarized transepithelial voltage (VT) in a concentration-dependent manner with a maximal effect at 1 μM. None of the other EET regioisomers (8,9-, 11,12-, or 14,15-EET; all at 1 μM) affected VT. This action was also stereoselective, with 5(S),6(R)-EET producing a 2.5-fold greater effect on VT than 5(R),6(S)-EET (1 μM each). Like basolateral prostaglandin E2 (PGE2), both luminal and basolateral 5,6-EET increased cytosolic Ca2+ concentration ([Ca2+]i) in the rabbit CCD. Pretreatment with cyclooxygenase inhibitors (10 μM ibuprofen or 5 μM indomethacin) completely blocked both the [Ca2+]i increase and the change in VT. Neither 5,6-epoxy-PGE1 nor 5-hydroxy-PGI1, cyclooxygenase metabolites of 5,6-EET, affected VT. However, when added to primary cultures of rabbit CCDs, 5,6-EET stimulated endogenous PGE2 synthesis. We propose that 5,6-EET stimulates endogenous prostaglandin synthesis, which inhibits electrogenic ion transport in the CCD.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Renal Fluid and Electrolyte Physiology |
| Volume | 268 |
| Issue number | 5 37-5 |
| State | Published - May 1995 |
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Keywords
- 5,6-epoxyeicosatrienoic acid
- Arachidonate
- Cytochrome P-450
- Kidney
- Sodium
ASJC Scopus subject areas
- Physiology
Cite this
5,6-EET inhibits ion transport in collecting duct by stimulating endogenous prostaglandin synthesis. / Sakairi, Yasunori; Jacobson, Harry R.; Noland, Thomas D.; Capdevila, Jorge H.; Falck, John R.; Breyer, Matthew D.
In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 268, No. 5 37-5, 05.1995.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 5,6-EET inhibits ion transport in collecting duct by stimulating endogenous prostaglandin synthesis
AU - Sakairi, Yasunori
AU - Jacobson, Harry R.
AU - Noland, Thomas D.
AU - Capdevila, Jorge H.
AU - Falck, John R.
AU - Breyer, Matthew D.
PY - 1995/5
Y1 - 1995/5
N2 - We examined the mechanism by which the cytochrome P-450 metabolite of arachidonate, 5,6-epoxyeicosatrienoic acid (5,6-EET), modulates electrogenic transport in the rabbit cortical collecting duct (CCD). 5,6-EET depolarized transepithelial voltage (VT) in a concentration-dependent manner with a maximal effect at 1 μM. None of the other EET regioisomers (8,9-, 11,12-, or 14,15-EET; all at 1 μM) affected VT. This action was also stereoselective, with 5(S),6(R)-EET producing a 2.5-fold greater effect on VT than 5(R),6(S)-EET (1 μM each). Like basolateral prostaglandin E2 (PGE2), both luminal and basolateral 5,6-EET increased cytosolic Ca2+ concentration ([Ca2+]i) in the rabbit CCD. Pretreatment with cyclooxygenase inhibitors (10 μM ibuprofen or 5 μM indomethacin) completely blocked both the [Ca2+]i increase and the change in VT. Neither 5,6-epoxy-PGE1 nor 5-hydroxy-PGI1, cyclooxygenase metabolites of 5,6-EET, affected VT. However, when added to primary cultures of rabbit CCDs, 5,6-EET stimulated endogenous PGE2 synthesis. We propose that 5,6-EET stimulates endogenous prostaglandin synthesis, which inhibits electrogenic ion transport in the CCD.
AB - We examined the mechanism by which the cytochrome P-450 metabolite of arachidonate, 5,6-epoxyeicosatrienoic acid (5,6-EET), modulates electrogenic transport in the rabbit cortical collecting duct (CCD). 5,6-EET depolarized transepithelial voltage (VT) in a concentration-dependent manner with a maximal effect at 1 μM. None of the other EET regioisomers (8,9-, 11,12-, or 14,15-EET; all at 1 μM) affected VT. This action was also stereoselective, with 5(S),6(R)-EET producing a 2.5-fold greater effect on VT than 5(R),6(S)-EET (1 μM each). Like basolateral prostaglandin E2 (PGE2), both luminal and basolateral 5,6-EET increased cytosolic Ca2+ concentration ([Ca2+]i) in the rabbit CCD. Pretreatment with cyclooxygenase inhibitors (10 μM ibuprofen or 5 μM indomethacin) completely blocked both the [Ca2+]i increase and the change in VT. Neither 5,6-epoxy-PGE1 nor 5-hydroxy-PGI1, cyclooxygenase metabolites of 5,6-EET, affected VT. However, when added to primary cultures of rabbit CCDs, 5,6-EET stimulated endogenous PGE2 synthesis. We propose that 5,6-EET stimulates endogenous prostaglandin synthesis, which inhibits electrogenic ion transport in the CCD.
KW - 5,6-epoxyeicosatrienoic acid
KW - Arachidonate
KW - Cytochrome P-450
KW - Kidney
KW - Sodium
UR - http://www.scopus.com/inward/record.url?scp=0029059860&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029059860&partnerID=8YFLogxK
M3 - Article
VL - 268
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
SN - 0363-6135
IS - 5 37-5
ER -