5,6-epoxyeicosatrienoic acid mobilizes Ca2+ in anterior pituitary cells

G. Snyder, F. Lattanzio, P. Yadagiri, J R Falck, J. Capdevila

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

Luteinizing hormone releasing hormone stimulates the concomitant release of luteinizing hormone and 45Ca2+ from prelabeled anterior pituitary cells. Indomethacin (10 μM) and nordihydroguaiaretic acid (10 μM) had no effect on the luteinizing hormone releasing hormone-stimulated release of either luteinizing hormone or 45Ca2+. Eicosatetraynoic acid (10 μM) blocked both luteinizing hormone releasing hormone-stimulated luteinizing hormone secretion and luteinizing hormone releasing hormone-stimulated 45Ca2+ efflux. 5,6-Epoxyeicosatrienoic acid stimulated both luteinizing hormone secretion and 45Ca2+ efflux from anterior pituitary cells. Additionally, 5,6-epoxyeicosatrienoic acid closely mimics the ability of luteinizing hormone releasing hormone to increase intracellular free calcium. These results are consistent with the hypothesis that 5,6-EET alters calcium homeostasis in a manner similar to that observed during luteinizing hormone releasing hormone stimulation of luteinizing hormone release.

Original languageEnglish (US)
Pages (from-to)1188-1194
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume139
Issue number3
DOIs
StatePublished - Sep 30 1986

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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