6-Fluoropyridoxol: A novel probe of cellular pH using 19F NMR spectroscopy

Vimal D. Mehta, Padmakar V. Kulkarni, Ralph P. Mason, Anca Constantinescu, Sivasubramanian Aravind, Naresh Goomer, Peter P. Antich

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

6-Fluoropyridoxol was evaluated as an intracellular pH indicator. This molecule exhibits exceptional sensitivity to changes in pH (∼ 10 ppm acid/base shift) and a pKa ∼8.2 appropriate for physiological investigations. Using 19F NMR spectroscopy we determined both intra- and extracellular pH in whole blood and confirmed the measurements using traditional techniques: ion-electrodes and 31P NMR spectroscopy.

Original languageEnglish (US)
Pages (from-to)234-238
Number of pages5
JournalFEBS Letters
Volume349
Issue number2
DOIs
StatePublished - Aug 1 1994

Keywords

  • Blood
  • F NMR
  • Fluoropyridoxol
  • pH

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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