Abstract
6-Fluoropyridoxol was evaluated as an intracellular pH indicator. This molecule exhibits exceptional sensitivity to changes in pH (∼ 10 ppm acid/base shift) and a pKa ∼8.2 appropriate for physiological investigations. Using 19F NMR spectroscopy we determined both intra- and extracellular pH in whole blood and confirmed the measurements using traditional techniques: ion-electrodes and 31P NMR spectroscopy.
Original language | English (US) |
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Pages (from-to) | 234-238 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 349 |
Issue number | 2 |
DOIs | |
State | Published - Aug 1 1994 |
Keywords
- Blood
- F NMR
- Fluoropyridoxol
- pH
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology