6-Fluoropyridoxol: A novel probe of cellular pH using 19F NMR spectroscopy

Vimal D. Mehta; Padmakar V. Kulkarni; Ralph P. Mason; Anca Constantinescu; Sivasubramanian Aravind; Naresh Goomer; Peter P. Antich

doi:10.1016/0014-5793(94)00675-X

6-Fluoropyridoxol: A novel probe of cellular pH using ¹⁹F NMR spectroscopy

Vimal D. Mehta, Padmakar V. Kulkarni, Ralph P. Mason, Anca Constantinescu, Sivasubramanian Aravind, Naresh Goomer, Peter P. Antich

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

6-Fluoropyridoxol was evaluated as an intracellular pH indicator. This molecule exhibits exceptional sensitivity to changes in pH (∼ 10 ppm acid/base shift) and a pK_a ∼8.2 appropriate for physiological investigations. Using ¹⁹F NMR spectroscopy we determined both intra- and extracellular pH in whole blood and confirmed the measurements using traditional techniques: ion-electrodes and ³¹P NMR spectroscopy.

Original language	English (US)
Pages (from-to)	234-238
Number of pages	5
Journal	FEBS Letters
Volume	349
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(94)00675-X
State	Published - Aug 1 1994

Keywords

Blood
F NMR
Fluoropyridoxol
pH

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(94)00675-X

Cite this

@article{e6a15b8d17224a64a8d389fa456d4d7a,

title = "6-Fluoropyridoxol: A novel probe of cellular pH using 19F NMR spectroscopy",

abstract = "6-Fluoropyridoxol was evaluated as an intracellular pH indicator. This molecule exhibits exceptional sensitivity to changes in pH (∼ 10 ppm acid/base shift) and a pKa ∼8.2 appropriate for physiological investigations. Using 19F NMR spectroscopy we determined both intra- and extracellular pH in whole blood and confirmed the measurements using traditional techniques: ion-electrodes and 31P NMR spectroscopy.",

keywords = "Blood, F NMR, Fluoropyridoxol, pH",

author = "Mehta, {Vimal D.} and Kulkarni, {Padmakar V.} and Mason, {Ralph P.} and Anca Constantinescu and Sivasubramanian Aravind and Naresh Goomer and Antich, {Peter P.}",

note = "Funding Information: Acknowledgements: This work was supported by Dallas Biomedical Corp./Hartford Foundation and a grant from the Texas Higher Education Coordinating Board (ATP 003660032); NMR experiments were performed at the Mary Nell and Ralph B. Rogers MR Center using Facilities supported by NIH Biotechnology Facility Grant #5-P41-RR02584. We are grateful to George Gorrie for preliminary NMR investigations.",

year = "1994",

month = aug,

day = "1",

doi = "10.1016/0014-5793(94)00675-X",

language = "English (US)",

volume = "349",

pages = "234--238",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - 6-Fluoropyridoxol

T2 - A novel probe of cellular pH using 19F NMR spectroscopy

AU - Mehta, Vimal D.

AU - Kulkarni, Padmakar V.

AU - Mason, Ralph P.

AU - Constantinescu, Anca

AU - Aravind, Sivasubramanian

AU - Goomer, Naresh

AU - Antich, Peter P.

N1 - Funding Information: Acknowledgements: This work was supported by Dallas Biomedical Corp./Hartford Foundation and a grant from the Texas Higher Education Coordinating Board (ATP 003660032); NMR experiments were performed at the Mary Nell and Ralph B. Rogers MR Center using Facilities supported by NIH Biotechnology Facility Grant #5-P41-RR02584. We are grateful to George Gorrie for preliminary NMR investigations.

PY - 1994/8/1

Y1 - 1994/8/1

N2 - 6-Fluoropyridoxol was evaluated as an intracellular pH indicator. This molecule exhibits exceptional sensitivity to changes in pH (∼ 10 ppm acid/base shift) and a pKa ∼8.2 appropriate for physiological investigations. Using 19F NMR spectroscopy we determined both intra- and extracellular pH in whole blood and confirmed the measurements using traditional techniques: ion-electrodes and 31P NMR spectroscopy.

AB - 6-Fluoropyridoxol was evaluated as an intracellular pH indicator. This molecule exhibits exceptional sensitivity to changes in pH (∼ 10 ppm acid/base shift) and a pKa ∼8.2 appropriate for physiological investigations. Using 19F NMR spectroscopy we determined both intra- and extracellular pH in whole blood and confirmed the measurements using traditional techniques: ion-electrodes and 31P NMR spectroscopy.

KW - Blood

KW - F NMR

KW - Fluoropyridoxol

KW - pH

UR - http://www.scopus.com/inward/record.url?scp=0027989868&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027989868&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(94)00675-X

DO - 10.1016/0014-5793(94)00675-X

M3 - Article

C2 - 8050572

AN - SCOPUS:0027989868

SN - 0014-5793

VL - 349

SP - 234

EP - 238

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -