A σ 1 region important for hemagglutination by serotype 3 reovirus strains

Terence S. Dermody, Max L. Nibert, Rhonda Bassel-Duby, Bernard N. Fields

Research output: Contribution to journalArticle

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Abstract

Hemagglutination (HA) by the mammalian reoviruses is mediated by interactions between the viral σ 1 protein and sialoglycoproteins on the erythrocyte surface. Three serotype 3 (T3) reovirus strains were identified that do not agglutinate either bovine or type O human erythrocytes (HA negative): T3 clone 43 (T3C43), T3 clone 44 (T3C44), and T3 clone 84 (T3C84). These three strains also showed a diminished capacity to bind the major erythrocyte sialoglycoprotein, glycophorin, in an enzyme-linked immunosorbent assay. To determine the molecular basis for these findings, we examined the deduced σ 1 amino acid sequences of the three HA-negative T3 strains and four HA-positive T3 strains. The limited number of sequence differences in the σ 1 proteins of these seven strains allowed us to identify single unique amino acid residues in each of the HA-negative strains (aspartate 198 in T3C43, leucine 204 in T3C44, and tryptophan 202 in T3C84) that cluster within a discrete region of the σ 1 tail. The identification of σ 1 residues important for HA and glycophorin binding suggests that tail-forming sequences are exposed on the virion surface, where they interact with carbohydrate residues on the surface of cells.

Original languageEnglish (US)
Pages (from-to)5173-5176
Number of pages4
JournalJournal of Virology
Volume64
Issue number10
StatePublished - 1990

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Mammalian orthoreovirus 3
Reoviridae
Hemagglutination
hemagglutination
Glycophorin
serotypes
Clone Cells
clones
triiodothyronine
erythrocytes
Tail
tail
Viral Proteins
aspartic acid
virion
Aspartic Acid
Leucine
Tryptophan
Virion
tryptophan

ASJC Scopus subject areas

  • Immunology

Cite this

Dermody, T. S., Nibert, M. L., Bassel-Duby, R., & Fields, B. N. (1990). A σ 1 region important for hemagglutination by serotype 3 reovirus strains. Journal of Virology, 64(10), 5173-5176.

A σ 1 region important for hemagglutination by serotype 3 reovirus strains. / Dermody, Terence S.; Nibert, Max L.; Bassel-Duby, Rhonda; Fields, Bernard N.

In: Journal of Virology, Vol. 64, No. 10, 1990, p. 5173-5176.

Research output: Contribution to journalArticle

Dermody, TS, Nibert, ML, Bassel-Duby, R & Fields, BN 1990, 'A σ 1 region important for hemagglutination by serotype 3 reovirus strains', Journal of Virology, vol. 64, no. 10, pp. 5173-5176.
Dermody, Terence S. ; Nibert, Max L. ; Bassel-Duby, Rhonda ; Fields, Bernard N. / A σ 1 region important for hemagglutination by serotype 3 reovirus strains. In: Journal of Virology. 1990 ; Vol. 64, No. 10. pp. 5173-5176.
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