A 200 kDa protein is associated with haemagglutinating isolates of Moraxella (Branhamella) catarrhalis

Margaret Fitzgerald, Riona Mulcahy, Susan Murphy, Conor Keane, Davis Coakley, Thomas Scott

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Moraxella catarrhalis adheres to human erythrocytes by means of a proteinaceous, trypsin sensitive, heat modifiable haemagglutinin. A 200 kDa protein was found to be associated with haemagglutinating isolates of M. catarrhalis. This protein was present on all haemagglutinating isolates (n = 17), but was absent on the non-haemagglutinating isolates (n = 23) examined. This protein demonstrated heat-modifiable properties in sodium dodecyl sulfate and was degraded by trypsin. Immunoblot assays with polyclonal antiserum indicated that the 200 kDa protein was associated exclusively with haemagglutinating isolates and antibodies to this protein did not recognise epitopes on non-haemagglutinating isolates. This protein, which appears to be a surface expressed protein may be a haemagglutinin of M. catarrhalis.

Original languageEnglish (US)
Pages (from-to)209-216
Number of pages8
JournalFEMS Immunology and Medical Microbiology
Volume18
Issue number3
DOIs
StatePublished - Jul 1 1997

Fingerprint

Moraxella (Branhamella) catarrhalis
Proteins
Hemagglutinins
Trypsin
Hot Temperature
Sodium Dodecyl Sulfate
Immune Sera
Epitopes
Membrane Proteins
Erythrocytes
Antibodies

Keywords

  • Haemagglutination
  • Heat modifiability
  • Immunoblotting
  • Moraxella (Branhamella) catarrhalis
  • Surface protein

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

A 200 kDa protein is associated with haemagglutinating isolates of Moraxella (Branhamella) catarrhalis. / Fitzgerald, Margaret; Mulcahy, Riona; Murphy, Susan; Keane, Conor; Coakley, Davis; Scott, Thomas.

In: FEMS Immunology and Medical Microbiology, Vol. 18, No. 3, 01.07.1997, p. 209-216.

Research output: Contribution to journalArticle

Fitzgerald, Margaret ; Mulcahy, Riona ; Murphy, Susan ; Keane, Conor ; Coakley, Davis ; Scott, Thomas. / A 200 kDa protein is associated with haemagglutinating isolates of Moraxella (Branhamella) catarrhalis. In: FEMS Immunology and Medical Microbiology. 1997 ; Vol. 18, No. 3. pp. 209-216.
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