A 29-kDa protein associated with p67phox expresses both peroxiredoxin and phospholipase A2 activity and enhances superoxide anion production by a cell-free system of NADPH oxidase activity

Patrick J. Leavey, Carolina Gonzalez-Aller, Gail Thurman, Michael Kleinberg, Lori Rinckel, Daniel W. Ambruso, Stefanie Freeman, Frans A. Kuypers, Daniel R. Ambruso

Research output: Contribution to journalArticle

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Abstract

Production of toxic oxygen metabolites provides a mechanism for microbicidal activity of the neutrophil. The NADPH oxidase enzyme system initiates the production of oxygen metabolites by reducing oxygen to form superoxide anion (O2.-). With stimulation of the respiratory burst, cytosolic oxidase components, p47phox, p67phox, and Rac, translocate to the phagolysomal and plasma membranes where they form a complex with cytochrome b558 and express enzyme activity. A 29-kDa neutrophil protein (p29) was identified by co-immunoprecipitation with p67phox. N-terminal sequence analysis of p29 revealed homology to an open reading frame gene described in a myeloid leukemia cell line. A cDNA for p29 identical to the open reading frame protein was amplified from RNA of neutrophils. Significant interaction between p29 and p67phox was demonstrated using a yeast two-hybrid system. A recombinant (rh) p29 was expressed in Sf9 cells resulting in a protein with an apparent molecular weight of 34,000. The rh-p29 showed immunoreactivity with the original rabbit antiserum that detected p47phox and p67phox. In addition, rh-p29 exhibited PLA2 activity, which was Ca2+ independent, optimal at low pH, and preferential for phosphatidylcholine substrates. The recombinant protein protected glutathione synthetase and directly inactivated H2O2. By activity and sequence homology, rh-p29 can be classified as a peroxiredoxin. Finally, O2.- production by plasma membrane and recombinant cytosolic oxidase components in the SDS-activated, cell-free NADPH oxidase system were enhanced by rh-p29. This effect was not inhibited by PLA2 inhibitors. Thus, p29 is a novel protein that associates with p67 and has peroxiredoxin activity. This protein has a potential role in protecting the NADPH oxidase by inactivating H2O2 or altering signaling pathways affected by H2O2.

Original languageEnglish (US)
Pages (from-to)45181-45187
Number of pages7
JournalJournal of Biological Chemistry
Volume277
Issue number47
DOIs
StatePublished - Nov 22 2002

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Peroxiredoxins
Cell-Free System
NADPH Oxidase
Phospholipases A2
Superoxides
Neutrophils
Cell membranes
Oxygen
Metabolites
Proteins
Open Reading Frames
Oxidoreductases
Glutathione Synthase
Cell Membrane
Sf9 Cells
Two-Hybrid System Techniques
Myeloid Leukemia
Poisons
Enzyme activity
Myeloid Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

A 29-kDa protein associated with p67phox expresses both peroxiredoxin and phospholipase A2 activity and enhances superoxide anion production by a cell-free system of NADPH oxidase activity. / Leavey, Patrick J.; Gonzalez-Aller, Carolina; Thurman, Gail; Kleinberg, Michael; Rinckel, Lori; Ambruso, Daniel W.; Freeman, Stefanie; Kuypers, Frans A.; Ambruso, Daniel R.

In: Journal of Biological Chemistry, Vol. 277, No. 47, 22.11.2002, p. 45181-45187.

Research output: Contribution to journalArticle

Leavey, Patrick J. ; Gonzalez-Aller, Carolina ; Thurman, Gail ; Kleinberg, Michael ; Rinckel, Lori ; Ambruso, Daniel W. ; Freeman, Stefanie ; Kuypers, Frans A. ; Ambruso, Daniel R. / A 29-kDa protein associated with p67phox expresses both peroxiredoxin and phospholipase A2 activity and enhances superoxide anion production by a cell-free system of NADPH oxidase activity. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 47. pp. 45181-45187.
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abstract = "Production of toxic oxygen metabolites provides a mechanism for microbicidal activity of the neutrophil. The NADPH oxidase enzyme system initiates the production of oxygen metabolites by reducing oxygen to form superoxide anion (O2.-). With stimulation of the respiratory burst, cytosolic oxidase components, p47phox, p67phox, and Rac, translocate to the phagolysomal and plasma membranes where they form a complex with cytochrome b558 and express enzyme activity. A 29-kDa neutrophil protein (p29) was identified by co-immunoprecipitation with p67phox. N-terminal sequence analysis of p29 revealed homology to an open reading frame gene described in a myeloid leukemia cell line. A cDNA for p29 identical to the open reading frame protein was amplified from RNA of neutrophils. Significant interaction between p29 and p67phox was demonstrated using a yeast two-hybrid system. A recombinant (rh) p29 was expressed in Sf9 cells resulting in a protein with an apparent molecular weight of 34,000. The rh-p29 showed immunoreactivity with the original rabbit antiserum that detected p47phox and p67phox. In addition, rh-p29 exhibited PLA2 activity, which was Ca2+ independent, optimal at low pH, and preferential for phosphatidylcholine substrates. The recombinant protein protected glutathione synthetase and directly inactivated H2O2. By activity and sequence homology, rh-p29 can be classified as a peroxiredoxin. Finally, O2.- production by plasma membrane and recombinant cytosolic oxidase components in the SDS-activated, cell-free NADPH oxidase system were enhanced by rh-p29. This effect was not inhibited by PLA2 inhibitors. Thus, p29 is a novel protein that associates with p67 and has peroxiredoxin activity. This protein has a potential role in protecting the NADPH oxidase by inactivating H2O2 or altering signaling pathways affected by H2O2.",
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T1 - A 29-kDa protein associated with p67phox expresses both peroxiredoxin and phospholipase A2 activity and enhances superoxide anion production by a cell-free system of NADPH oxidase activity

AU - Leavey, Patrick J.

AU - Gonzalez-Aller, Carolina

AU - Thurman, Gail

AU - Kleinberg, Michael

AU - Rinckel, Lori

AU - Ambruso, Daniel W.

AU - Freeman, Stefanie

AU - Kuypers, Frans A.

AU - Ambruso, Daniel R.

PY - 2002/11/22

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N2 - Production of toxic oxygen metabolites provides a mechanism for microbicidal activity of the neutrophil. The NADPH oxidase enzyme system initiates the production of oxygen metabolites by reducing oxygen to form superoxide anion (O2.-). With stimulation of the respiratory burst, cytosolic oxidase components, p47phox, p67phox, and Rac, translocate to the phagolysomal and plasma membranes where they form a complex with cytochrome b558 and express enzyme activity. A 29-kDa neutrophil protein (p29) was identified by co-immunoprecipitation with p67phox. N-terminal sequence analysis of p29 revealed homology to an open reading frame gene described in a myeloid leukemia cell line. A cDNA for p29 identical to the open reading frame protein was amplified from RNA of neutrophils. Significant interaction between p29 and p67phox was demonstrated using a yeast two-hybrid system. A recombinant (rh) p29 was expressed in Sf9 cells resulting in a protein with an apparent molecular weight of 34,000. The rh-p29 showed immunoreactivity with the original rabbit antiserum that detected p47phox and p67phox. In addition, rh-p29 exhibited PLA2 activity, which was Ca2+ independent, optimal at low pH, and preferential for phosphatidylcholine substrates. The recombinant protein protected glutathione synthetase and directly inactivated H2O2. By activity and sequence homology, rh-p29 can be classified as a peroxiredoxin. Finally, O2.- production by plasma membrane and recombinant cytosolic oxidase components in the SDS-activated, cell-free NADPH oxidase system were enhanced by rh-p29. This effect was not inhibited by PLA2 inhibitors. Thus, p29 is a novel protein that associates with p67 and has peroxiredoxin activity. This protein has a potential role in protecting the NADPH oxidase by inactivating H2O2 or altering signaling pathways affected by H2O2.

AB - Production of toxic oxygen metabolites provides a mechanism for microbicidal activity of the neutrophil. The NADPH oxidase enzyme system initiates the production of oxygen metabolites by reducing oxygen to form superoxide anion (O2.-). With stimulation of the respiratory burst, cytosolic oxidase components, p47phox, p67phox, and Rac, translocate to the phagolysomal and plasma membranes where they form a complex with cytochrome b558 and express enzyme activity. A 29-kDa neutrophil protein (p29) was identified by co-immunoprecipitation with p67phox. N-terminal sequence analysis of p29 revealed homology to an open reading frame gene described in a myeloid leukemia cell line. A cDNA for p29 identical to the open reading frame protein was amplified from RNA of neutrophils. Significant interaction between p29 and p67phox was demonstrated using a yeast two-hybrid system. A recombinant (rh) p29 was expressed in Sf9 cells resulting in a protein with an apparent molecular weight of 34,000. The rh-p29 showed immunoreactivity with the original rabbit antiserum that detected p47phox and p67phox. In addition, rh-p29 exhibited PLA2 activity, which was Ca2+ independent, optimal at low pH, and preferential for phosphatidylcholine substrates. The recombinant protein protected glutathione synthetase and directly inactivated H2O2. By activity and sequence homology, rh-p29 can be classified as a peroxiredoxin. Finally, O2.- production by plasma membrane and recombinant cytosolic oxidase components in the SDS-activated, cell-free NADPH oxidase system were enhanced by rh-p29. This effect was not inhibited by PLA2 inhibitors. Thus, p29 is a novel protein that associates with p67 and has peroxiredoxin activity. This protein has a potential role in protecting the NADPH oxidase by inactivating H2O2 or altering signaling pathways affected by H2O2.

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