A bcr3/Short form PML-RARα transcript in an acute promyelocytic leukemia resulted from a derivative chromosome 17 due to submicroscopic insertion of the PML gene into the RARα locus

Huan You Wang, Jiantao Ding, Mohammad A. Vasef, Kathleen S. Wilson

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Acute promyelocytic leukemia (APL) is a subtype of acute myeloid leukemia. Submicroscopic insertion of RARαinto PML, resulting in PML-RARα from derivative chromosome 15, has been rarely reported. Herein, we describe a functional PML-RARαtranscript from the long arm of derivative chromosome 17 in a patient with microgranular APL. The conventional karyotype showed normal chromosomes 15 and 17. It is interesting that interphase and metaphase fluorescence in situ hybridizations demonstrated a fusion signal on the long arm of one chromosome 17 homolog, with both PML and RARαstill present on chromosomes 15 and 17, respectively, although the signal on one chromosome 15 was weaker, indicating partial loss of the PML gene. Reverse transcriptase-polymerase chain reaction revealed a transcript corresponding to a break cluster region 3 (bcr3) short form PML-RARα To the best of our knowledge, this is the first report of an APL with a bcr3/short form PML-RARαtranscript generated from derivative chromosome 17 due to submicroscopic insertion of the PML gene into the RARαlocus.

Original languageEnglish (US)
Pages (from-to)64-71
Number of pages8
JournalAmerican Journal of Clinical Pathology
Volume131
Issue number1
DOIs
StatePublished - Jan 2009

Fingerprint

Acute Promyelocytic Leukemia
Chromosomes, Human, Pair 17
Insertional Mutagenesis
Chromosomes, Human, Pair 15
Interphase
Metaphase
Reverse Transcriptase Polymerase Chain Reaction
Fluorescence In Situ Hybridization
Karyotype
Acute Myeloid Leukemia
Genes

Keywords

  • Acute promyelocytic leukemia
  • Bcr3/short form pML-RARα
  • Derivative chromosome 17
  • Fluorescence in situ hybridization
  • Locus
  • PML gene insertion
  • RARα
  • Transcript

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

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title = "A bcr3/Short form PML-RARα transcript in an acute promyelocytic leukemia resulted from a derivative chromosome 17 due to submicroscopic insertion of the PML gene into the RARα locus",
abstract = "Acute promyelocytic leukemia (APL) is a subtype of acute myeloid leukemia. Submicroscopic insertion of RARαinto PML, resulting in PML-RARα from derivative chromosome 15, has been rarely reported. Herein, we describe a functional PML-RARαtranscript from the long arm of derivative chromosome 17 in a patient with microgranular APL. The conventional karyotype showed normal chromosomes 15 and 17. It is interesting that interphase and metaphase fluorescence in situ hybridizations demonstrated a fusion signal on the long arm of one chromosome 17 homolog, with both PML and RARαstill present on chromosomes 15 and 17, respectively, although the signal on one chromosome 15 was weaker, indicating partial loss of the PML gene. Reverse transcriptase-polymerase chain reaction revealed a transcript corresponding to a break cluster region 3 (bcr3) short form PML-RARα To the best of our knowledge, this is the first report of an APL with a bcr3/short form PML-RARαtranscript generated from derivative chromosome 17 due to submicroscopic insertion of the PML gene into the RARαlocus.",
keywords = "Acute promyelocytic leukemia, Bcr3/short form pML-RARα, Derivative chromosome 17, Fluorescence in situ hybridization, Locus, PML gene insertion, RARα, Transcript",
author = "Wang, {Huan You} and Jiantao Ding and Vasef, {Mohammad A.} and Wilson, {Kathleen S.}",
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T1 - A bcr3/Short form PML-RARα transcript in an acute promyelocytic leukemia resulted from a derivative chromosome 17 due to submicroscopic insertion of the PML gene into the RARα locus

AU - Wang, Huan You

AU - Ding, Jiantao

AU - Vasef, Mohammad A.

AU - Wilson, Kathleen S.

PY - 2009/1

Y1 - 2009/1

N2 - Acute promyelocytic leukemia (APL) is a subtype of acute myeloid leukemia. Submicroscopic insertion of RARαinto PML, resulting in PML-RARα from derivative chromosome 15, has been rarely reported. Herein, we describe a functional PML-RARαtranscript from the long arm of derivative chromosome 17 in a patient with microgranular APL. The conventional karyotype showed normal chromosomes 15 and 17. It is interesting that interphase and metaphase fluorescence in situ hybridizations demonstrated a fusion signal on the long arm of one chromosome 17 homolog, with both PML and RARαstill present on chromosomes 15 and 17, respectively, although the signal on one chromosome 15 was weaker, indicating partial loss of the PML gene. Reverse transcriptase-polymerase chain reaction revealed a transcript corresponding to a break cluster region 3 (bcr3) short form PML-RARα To the best of our knowledge, this is the first report of an APL with a bcr3/short form PML-RARαtranscript generated from derivative chromosome 17 due to submicroscopic insertion of the PML gene into the RARαlocus.

AB - Acute promyelocytic leukemia (APL) is a subtype of acute myeloid leukemia. Submicroscopic insertion of RARαinto PML, resulting in PML-RARα from derivative chromosome 15, has been rarely reported. Herein, we describe a functional PML-RARαtranscript from the long arm of derivative chromosome 17 in a patient with microgranular APL. The conventional karyotype showed normal chromosomes 15 and 17. It is interesting that interphase and metaphase fluorescence in situ hybridizations demonstrated a fusion signal on the long arm of one chromosome 17 homolog, with both PML and RARαstill present on chromosomes 15 and 17, respectively, although the signal on one chromosome 15 was weaker, indicating partial loss of the PML gene. Reverse transcriptase-polymerase chain reaction revealed a transcript corresponding to a break cluster region 3 (bcr3) short form PML-RARα To the best of our knowledge, this is the first report of an APL with a bcr3/short form PML-RARαtranscript generated from derivative chromosome 17 due to submicroscopic insertion of the PML gene into the RARαlocus.

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KW - Transcript

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