A bifunctional intronic element regulates the expression of the arginine/lysine transporter Cat-1 via mechanisms involving the purine-rich element binding protein A (Purα)

Charlie C. Huang, Calin Bogdan Chiribau, Mithu Majumder, Cheng Ming Chiang, Ronald C. Wek, Robert J. Kelm, Kamel Khalili, Martin D. Snider, Maria Hatzoglou

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Expression of the arginine/lysine transporter Cat-1 is highly induced in proliferating and stressed cells via mechanisms that include transcriptional activation. A bifunctional INE (intronic element) within the first intron of the Cat-1 gene was identified and characterized in this study. The INE had high sequence homology to an amino acid response element and was shown to act as a transcriptional enhancer in unstressed cells by binding the transcription factor, purine-rich element binding protein A (Pura). During endoplasmic reticulum stress, binding of Purα to the INE decreased; the element acted as a positive regulator in early stress by binding of the transcription factor ATF4 and as a negative regulator in prolonged stress by binding the stress-induced C/EBP family member, CHOP.Weconclude that transcriptional control of the Cat-1 gene is tightly controlled by multiple cis-DNA elements, contributing to regulation of cationic amino acid transport for cell growth and proliferation. In addition, we propose that genes may use stress-response elements such as the INE to support basal expression in the absence of stress.

Original languageEnglish (US)
Pages (from-to)32312-32320
Number of pages9
JournalJournal of Biological Chemistry
Volume284
Issue number47
DOIs
StatePublished - Nov 20 2009

Fingerprint

Staphylococcal Protein A
Lysine
Arginine
Carrier Proteins
Cats
Response Elements
Activating Transcription Factor 4
Genes
Amino Acid Sequence Homology
Endoplasmic Reticulum Stress
Introns
Transcriptional Activation
Transcription Factors
Cell Proliferation
Amino Acids
DNA
Cell proliferation
Cell growth
Growth
purine

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

A bifunctional intronic element regulates the expression of the arginine/lysine transporter Cat-1 via mechanisms involving the purine-rich element binding protein A (Purα). / Huang, Charlie C.; Chiribau, Calin Bogdan; Majumder, Mithu; Chiang, Cheng Ming; Wek, Ronald C.; Kelm, Robert J.; Khalili, Kamel; Snider, Martin D.; Hatzoglou, Maria.

In: Journal of Biological Chemistry, Vol. 284, No. 47, 20.11.2009, p. 32312-32320.

Research output: Contribution to journalArticle

Huang, Charlie C. ; Chiribau, Calin Bogdan ; Majumder, Mithu ; Chiang, Cheng Ming ; Wek, Ronald C. ; Kelm, Robert J. ; Khalili, Kamel ; Snider, Martin D. ; Hatzoglou, Maria. / A bifunctional intronic element regulates the expression of the arginine/lysine transporter Cat-1 via mechanisms involving the purine-rich element binding protein A (Purα). In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 47. pp. 32312-32320.
@article{92dadbc8f7394ee98247b5cd95ee8323,
title = "A bifunctional intronic element regulates the expression of the arginine/lysine transporter Cat-1 via mechanisms involving the purine-rich element binding protein A (Purα)",
abstract = "Expression of the arginine/lysine transporter Cat-1 is highly induced in proliferating and stressed cells via mechanisms that include transcriptional activation. A bifunctional INE (intronic element) within the first intron of the Cat-1 gene was identified and characterized in this study. The INE had high sequence homology to an amino acid response element and was shown to act as a transcriptional enhancer in unstressed cells by binding the transcription factor, purine-rich element binding protein A (Pura). During endoplasmic reticulum stress, binding of Purα to the INE decreased; the element acted as a positive regulator in early stress by binding of the transcription factor ATF4 and as a negative regulator in prolonged stress by binding the stress-induced C/EBP family member, CHOP.Weconclude that transcriptional control of the Cat-1 gene is tightly controlled by multiple cis-DNA elements, contributing to regulation of cationic amino acid transport for cell growth and proliferation. In addition, we propose that genes may use stress-response elements such as the INE to support basal expression in the absence of stress.",
author = "Huang, {Charlie C.} and Chiribau, {Calin Bogdan} and Mithu Majumder and Chiang, {Cheng Ming} and Wek, {Ronald C.} and Kelm, {Robert J.} and Kamel Khalili and Snider, {Martin D.} and Maria Hatzoglou",
year = "2009",
month = "11",
day = "20",
doi = "10.1074/jbc.M109.024471",
language = "English (US)",
volume = "284",
pages = "32312--32320",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "47",

}

TY - JOUR

T1 - A bifunctional intronic element regulates the expression of the arginine/lysine transporter Cat-1 via mechanisms involving the purine-rich element binding protein A (Purα)

AU - Huang, Charlie C.

AU - Chiribau, Calin Bogdan

AU - Majumder, Mithu

AU - Chiang, Cheng Ming

AU - Wek, Ronald C.

AU - Kelm, Robert J.

AU - Khalili, Kamel

AU - Snider, Martin D.

AU - Hatzoglou, Maria

PY - 2009/11/20

Y1 - 2009/11/20

N2 - Expression of the arginine/lysine transporter Cat-1 is highly induced in proliferating and stressed cells via mechanisms that include transcriptional activation. A bifunctional INE (intronic element) within the first intron of the Cat-1 gene was identified and characterized in this study. The INE had high sequence homology to an amino acid response element and was shown to act as a transcriptional enhancer in unstressed cells by binding the transcription factor, purine-rich element binding protein A (Pura). During endoplasmic reticulum stress, binding of Purα to the INE decreased; the element acted as a positive regulator in early stress by binding of the transcription factor ATF4 and as a negative regulator in prolonged stress by binding the stress-induced C/EBP family member, CHOP.Weconclude that transcriptional control of the Cat-1 gene is tightly controlled by multiple cis-DNA elements, contributing to regulation of cationic amino acid transport for cell growth and proliferation. In addition, we propose that genes may use stress-response elements such as the INE to support basal expression in the absence of stress.

AB - Expression of the arginine/lysine transporter Cat-1 is highly induced in proliferating and stressed cells via mechanisms that include transcriptional activation. A bifunctional INE (intronic element) within the first intron of the Cat-1 gene was identified and characterized in this study. The INE had high sequence homology to an amino acid response element and was shown to act as a transcriptional enhancer in unstressed cells by binding the transcription factor, purine-rich element binding protein A (Pura). During endoplasmic reticulum stress, binding of Purα to the INE decreased; the element acted as a positive regulator in early stress by binding of the transcription factor ATF4 and as a negative regulator in prolonged stress by binding the stress-induced C/EBP family member, CHOP.Weconclude that transcriptional control of the Cat-1 gene is tightly controlled by multiple cis-DNA elements, contributing to regulation of cationic amino acid transport for cell growth and proliferation. In addition, we propose that genes may use stress-response elements such as the INE to support basal expression in the absence of stress.

UR - http://www.scopus.com/inward/record.url?scp=70450257666&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70450257666&partnerID=8YFLogxK

U2 - 10.1074/jbc.M109.024471

DO - 10.1074/jbc.M109.024471

M3 - Article

VL - 284

SP - 32312

EP - 32320

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 47

ER -