A candidate target for G protein action in brain

Linda T. Chen, Alfred G. Gilman, Tohru Kozasa

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

An effector candidate for G protein action, GRIN1, was identified by screening a cDNA expression library with phosphorylated GTPγS-G(z)α as a probe. GRIN1 is a novel protein without substantial homology to known protein domains. It is expressed largely in brain and binds specifically to activated G(z)α, G(o)α, and G(i)α through its carboxyl-terminal region. The protein KIAA0514 (GRIN2) is homologous to GRIN1 at its carboxyl terminus and also binds to activated G(o)α. Both GRIN1 and G(o)α are membrane-bound proteins that are enriched in the growth cones of neurites. Coexpression of GRIN1 or GRIN2 with activated G(o)α causes formation of a network of fine processes in Neuro2a cells, suggesting that these pathways may function downstream of G(o)α to control growth of neurites.

Original languageEnglish (US)
Pages (from-to)26931-26938
Number of pages8
JournalJournal of Biological Chemistry
Volume274
Issue number38
DOIs
StatePublished - Sep 17 1999

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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