A carbon nanotube-based Raman-imaging immunoassay for evaluating tumor targeting ligands

Pooja Bajaj, Carole Mikoryak, Ruhung Wang, David K. Bushdiecker, Pauras Memon, Rockford K. Draper, Gregg R. Dieckmann, Paul Pantano, Inga H. Musselman

Research output: Contribution to journalArticle

Abstract

Herein, we describe a versatile immunoassay that uses biotinylated single-walled carbon nanotubes (SWNTs) as a Raman label, avidin-biotin chemistry to link targeting ligands to the label, and confocal Raman microscopy to image whole cells. Using a breast tumor cell model, we demonstrate the usefulness of the method to assess membrane receptor/ligand systems by evaluating a monoclonal antibody, Her-66, known to target the Her2 receptors that are overexpressed on these cells. We present two-dimensional Raman images of the cellular distribution of the SWNT labels corresponding to the distribution of the Her2 receptors in different focal planes through the cell with validation of the method using immunofluorescence microscopy, demonstrating that the Her-66-SWNT complexes were targeted to Her2 cell receptors.

Original languageEnglish (US)
Pages (from-to)3069-3076
Number of pages8
JournalAnalyst
Volume139
Issue number12
DOIs
StatePublished - May 19 2014
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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  • Cite this

    Bajaj, P., Mikoryak, C., Wang, R., Bushdiecker, D. K., Memon, P., Draper, R. K., Dieckmann, G. R., Pantano, P., & Musselman, I. H. (2014). A carbon nanotube-based Raman-imaging immunoassay for evaluating tumor targeting ligands. Analyst, 139(12), 3069-3076. https://doi.org/10.1039/c4an00258j