A cDNA clone encoding neuropeptides isolated from Aplysia neuron L11

R. Taussig, R. R. Kaldany, R. H. Scheller

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Single nerve cells can use more than one substance as extracellular chemical messengers. Classical transmitters have been shown to coexist in the same neuron and possibly even in the same vesicle as neuroactive peptides. Furthermore, multiple neuroactive peptides, which are thought to be coreleased, are often encoded in the same precursor assuring stoichiometric synthesis. The precise organization of multiple message systems and the physiological significance of the coexistence is poorly understood. The abdominal ganglion of the gastropod mollusc Aplysia contains a number of identified neurons that are cotransmitter candidates. One such cell, L11, is cholinergic and probably also uses biologically active peptides. Differential screening with labeled cDNA was used to isolate cDNA clones expressed specifically in the bag cells and abdominal ganglion neurons L11 or R15. Analysis of an L11-specific clone suggests that it encodes a 14.7-kDa protein that is the precursor for the secreted peptides. The poly(A)+ RNA transcript is ~1.2 kilobases and there are 1-3 copies of this gene in the Aplysia haploid genome.

Original languageEnglish (US)
Pages (from-to)4988-4992
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number15 I
StatePublished - 1984

Fingerprint

Aplysia
Neuropeptides
Complementary DNA
Clone Cells
Neurons
Peptides
Ganglia
Gastropoda
Protein Precursors
Mollusca
Haploidy
Cholinergic Agents
Genome
Messenger RNA
Genes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

A cDNA clone encoding neuropeptides isolated from Aplysia neuron L11. / Taussig, R.; Kaldany, R. R.; Scheller, R. H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, No. 15 I, 1984, p. 4988-4992.

Research output: Contribution to journalArticle

@article{b304d3275d334a609a7cd2916b00f999,
title = "A cDNA clone encoding neuropeptides isolated from Aplysia neuron L11",
abstract = "Single nerve cells can use more than one substance as extracellular chemical messengers. Classical transmitters have been shown to coexist in the same neuron and possibly even in the same vesicle as neuroactive peptides. Furthermore, multiple neuroactive peptides, which are thought to be coreleased, are often encoded in the same precursor assuring stoichiometric synthesis. The precise organization of multiple message systems and the physiological significance of the coexistence is poorly understood. The abdominal ganglion of the gastropod mollusc Aplysia contains a number of identified neurons that are cotransmitter candidates. One such cell, L11, is cholinergic and probably also uses biologically active peptides. Differential screening with labeled cDNA was used to isolate cDNA clones expressed specifically in the bag cells and abdominal ganglion neurons L11 or R15. Analysis of an L11-specific clone suggests that it encodes a 14.7-kDa protein that is the precursor for the secreted peptides. The poly(A)+ RNA transcript is ~1.2 kilobases and there are 1-3 copies of this gene in the Aplysia haploid genome.",
author = "R. Taussig and Kaldany, {R. R.} and Scheller, {R. H.}",
year = "1984",
language = "English (US)",
volume = "81",
pages = "4988--4992",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "15 I",

}

TY - JOUR

T1 - A cDNA clone encoding neuropeptides isolated from Aplysia neuron L11

AU - Taussig, R.

AU - Kaldany, R. R.

AU - Scheller, R. H.

PY - 1984

Y1 - 1984

N2 - Single nerve cells can use more than one substance as extracellular chemical messengers. Classical transmitters have been shown to coexist in the same neuron and possibly even in the same vesicle as neuroactive peptides. Furthermore, multiple neuroactive peptides, which are thought to be coreleased, are often encoded in the same precursor assuring stoichiometric synthesis. The precise organization of multiple message systems and the physiological significance of the coexistence is poorly understood. The abdominal ganglion of the gastropod mollusc Aplysia contains a number of identified neurons that are cotransmitter candidates. One such cell, L11, is cholinergic and probably also uses biologically active peptides. Differential screening with labeled cDNA was used to isolate cDNA clones expressed specifically in the bag cells and abdominal ganglion neurons L11 or R15. Analysis of an L11-specific clone suggests that it encodes a 14.7-kDa protein that is the precursor for the secreted peptides. The poly(A)+ RNA transcript is ~1.2 kilobases and there are 1-3 copies of this gene in the Aplysia haploid genome.

AB - Single nerve cells can use more than one substance as extracellular chemical messengers. Classical transmitters have been shown to coexist in the same neuron and possibly even in the same vesicle as neuroactive peptides. Furthermore, multiple neuroactive peptides, which are thought to be coreleased, are often encoded in the same precursor assuring stoichiometric synthesis. The precise organization of multiple message systems and the physiological significance of the coexistence is poorly understood. The abdominal ganglion of the gastropod mollusc Aplysia contains a number of identified neurons that are cotransmitter candidates. One such cell, L11, is cholinergic and probably also uses biologically active peptides. Differential screening with labeled cDNA was used to isolate cDNA clones expressed specifically in the bag cells and abdominal ganglion neurons L11 or R15. Analysis of an L11-specific clone suggests that it encodes a 14.7-kDa protein that is the precursor for the secreted peptides. The poly(A)+ RNA transcript is ~1.2 kilobases and there are 1-3 copies of this gene in the Aplysia haploid genome.

UR - http://www.scopus.com/inward/record.url?scp=0021258575&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021258575&partnerID=8YFLogxK

M3 - Article

VL - 81

SP - 4988

EP - 4992

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 15 I

ER -