A cell-based screening system for anti-influenza A virus agents

Wan Ying Wong, Sheng Wei Loh, Wei Lun Ng, Ming Cheang Tan, Kok Siong Yeo, Chung Yeng Looi, Mohd Jamil Maah, Chee Kwee Ea

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Abstract

Emerging of drug resistant influenza A virus (IAV) has been a big challenge for anti-IAV therapy. In this study, we describe a relatively easy and safe cell-based screening system for anti-IAV replication inhibitors using a non-replicative strain of IAV. A nickel (II) complex of polyhydroxybenzaldehyde N4-thiosemicarbazone (NiPT5) was recently found to exhibit anti-inflammatory activity in vivo and in vitro. NiPT5 impedes the signaling cascades that lead to the activation of NF-κB in response to different stimuli, such as LPS and TNFα. Using our cell-based screening system, we report that pretreating cells with NiPT5 protects cells from influenza A virus (IAV) and vesicular stomatitis virus (VSV) infection. Furthermore, NiPT5 inhibits replication of IAV by inhibiting transcription and translation of vRNAs of IAV. Additionally, NiPT5 reduces IAV-induced type I interferon response and cytokines production. Moreover, NiPT5 prevents activation of NF-κB, and IRF3 in response to IAV infection. These results demonstrate that NiPT5 is a potent antiviral agent that inhibits the early phase of IAV replication.

Original languageEnglish (US)
Article number8672
JournalScientific reports
Volume5
DOIs
StatePublished - 2015

ASJC Scopus subject areas

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    Wong, W. Y., Loh, S. W., Ng, W. L., Tan, M. C., Yeo, K. S., Looi, C. Y., Maah, M. J., & Ea, C. K. (2015). A cell-based screening system for anti-influenza A virus agents. Scientific reports, 5, [8672]. https://doi.org/10.1038/srep08672