A complex of mammalian Ufd1 and Npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways

Hemmo H. Meyer, James G. Shorter, Joachim Seemann, Darryl Pappin, Graham Warren

Research output: Contribution to journalArticle

357 Citations (Scopus)

Abstract

The AAA-ATPase, p97/Cdc48p, has been implicated in many different pathways ranging from membrane fusion to ubiquitin-dependent protein degradation. Binding of the p47 complex directs p97 to act in the post-mitotic fusion of Golgi membranes. We now describe another binding complex comprising mammalian Ufd1 and Npl4. Yeast Ufd1p is required for ubiquitin-dependent protein degradation whereas yeast Npl4p has been implicated in nuclear transport. In rat liver cytosol, Ufd1 and Npl4 form a binary complex, which exists either alone or bound to p97. Ufd1\Npl4 competes with p47 for binding to p97 and so inhibits Golgi membrane fusion. This suggests that it is involved in another cellular function catalysed by p97, the most likely being ubiquitin-dependent events during mitosis. The fact that the binding of p47 and Ufd1\Npl4 is mutually exclusive suggests that these protein complexes act as adapters, directing a basic p97 activity into different cellular pathways.

Original languageEnglish (US)
Pages (from-to)2181-2192
Number of pages12
JournalEMBO Journal
Volume19
Issue number10
StatePublished - May 15 2000

Fingerprint

Membrane Fusion
Cell Nucleus Active Transport
Ubiquitin
Fusion reactions
Membranes
Yeast
Proteolysis
Yeasts
Degradation
Proteins
Mitosis
Liver
Cytosol
Rats
p97 ATPase

Keywords

  • AAA-ATPase
  • CDC48
  • DiGeorge
  • Ubiquitin
  • UFD1L

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

A complex of mammalian Ufd1 and Npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways. / Meyer, Hemmo H.; Shorter, James G.; Seemann, Joachim; Pappin, Darryl; Warren, Graham.

In: EMBO Journal, Vol. 19, No. 10, 15.05.2000, p. 2181-2192.

Research output: Contribution to journalArticle

Meyer, HH, Shorter, JG, Seemann, J, Pappin, D & Warren, G 2000, 'A complex of mammalian Ufd1 and Npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways', EMBO Journal, vol. 19, no. 10, pp. 2181-2192.
Meyer, Hemmo H. ; Shorter, James G. ; Seemann, Joachim ; Pappin, Darryl ; Warren, Graham. / A complex of mammalian Ufd1 and Npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways. In: EMBO Journal. 2000 ; Vol. 19, No. 10. pp. 2181-2192.
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