A composite bacteriophage alters colonization by an intestinal commensal bacterium

Breck A. Duerkop, Charmaine V. Clements, Darcy Rollins, Jorge L M Rodrigues, Lora V. Hooper

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

The mammalian intestine is home to a dense community of bacteria and its associated bacteriophage (phage). Virtually nothing is known about how phages impact the establishment and maintenance of resident bacterial communities in the intestine. Here, we examine the phages harbored by Enterococcus faecalis, a commensal of the human intestine. We show that E. faecalis strain V583 produces a composite phage (φV1/7) derived from two distinct chromosomally encoded prophage elements. One prophage, prophage 1 (φV1), encodes the structural genes necessary for phage particle production. Another prophage, prophage 7 (φV7), is required for phage infection of susceptible host bacteria. Production of φV1/7 is controlled, in part, by nutrient availability, because φV1/7 particle numbers are elevated by free amino acids in culture and during growth in the mouse intestine. φV1/7 confers an advantage to E. faecalis V583 during competition with other E. faecalis strains in vitro and in vivo. Thus, we propose that E. faecalis V583 uses phage particles to establish and maintain dominance of its intestinal niche in the presence of closely related competing strains. Our findings indicate that bacteriophages can impact the dynamics of bacterial colonization in the mammalian intestinal ecosystem.

Original languageEnglish (US)
Pages (from-to)17621-17626
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number43
DOIs
StatePublished - Oct 23 2012

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Bacteriophages
Prophages
Bacteria
Enterococcus faecalis
Intestines
Ecosystem
Maintenance
Amino Acids
Food
Growth
Infection
Genes

Keywords

  • Commensal bacteria
  • Enterococci
  • Microbiota
  • Phage predation

ASJC Scopus subject areas

  • General

Cite this

A composite bacteriophage alters colonization by an intestinal commensal bacterium. / Duerkop, Breck A.; Clements, Charmaine V.; Rollins, Darcy; Rodrigues, Jorge L M; Hooper, Lora V.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 43, 23.10.2012, p. 17621-17626.

Research output: Contribution to journalArticle

Duerkop, Breck A. ; Clements, Charmaine V. ; Rollins, Darcy ; Rodrigues, Jorge L M ; Hooper, Lora V. / A composite bacteriophage alters colonization by an intestinal commensal bacterium. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 43. pp. 17621-17626.
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