A computer program for displaying two-dimensional gel electrophoresis data

Albert Alexander, Bryan Cullen, Kirsten Emigholz, Michael V. Norgard, John J. Monahan

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

A computer program is described which facilitates comparison between the pattern of spots seen on different two-dimensional polyacrylamide electrophoresis gels. Essentially, the position of each spot is replotted on a graph by the computer using its molecular weight and isoelectric point as coordinates. An intensity factor is also assigned to each point by the operator which will determine the size and shape of the final plotted spot on the computer drawn figure. The resulting plot makes it more feasible to compare patterns of spots between independently run two-dimensional electrophoresis gels.

Original languageEnglish (US)
Pages (from-to)176-183
Number of pages8
JournalAnalytical biochemistry
Volume103
Issue number1
DOIs
StatePublished - Mar 15 1980

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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