A cytolytic human T lymphocyte clone differentially recognizing HLA-B27 subtypes

Yoshiki Miyachi, Siew Lin Wee, Li Kuang Chen, F. Carl Grumet, Robert J. Bowman, Joel D. Taurog

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Abstract

A cytolytic human T cell (CTL) clone, designated F/M-F159, has been produced, the lytic specificity of which distinguishes subtypes of HLA-B27. This was demonstrated in cell-mediated lympholysis (CML) assays of: 1) a panel of target cells from unrelated donors, 75 B27 + and 36 B27-; 2) six families, including 20 B27+ and 14 B27- individuals; and 3) B27+ and B27- variants of a B27+ lymphoblastoid cell line (LCL). Specificity of F/M-F159 for HLA-B27 was confirmed by blocking studies with monoclonal antibodies. Lysis of B27+ targets reactive with the anti-B27 monoclonal antibody B27M2 was 30-104%, while lysis of B27+,B27M2- targets was 4-22%. Lysis of B27- targets expressing HLA-Bw47, known to be cross-reactive with the B27M2 antibody, was 10 to 19%, while lysis of all other B27 - targets was ≤10%. Clone F/M-F159 lysed B27+ targets, and failed to lyse B27-targets, irrespective of the clinical status of the cell donors. It is concluded that F/M-F159 recognizes an epitope present on the majority of serologically identified HLA-B27 molecules and that this epitope is close related to, but not identical with, the epitope recognized by the antibody B27M2. These findings are interpreted as supporting a direct role for HLA-B27 in disease pathogenesis.

Original languageEnglish (US)
Pages (from-to)237-249
Number of pages13
JournalHuman Immunology
Volume10
Issue number4
DOIs
StatePublished - 1984

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HLA-B27 Antigen
Clone Cells
T-Lymphocytes
Epitopes
Monoclonal Antibodies
Unrelated Donors
Antibodies
Tissue Donors
Cell Line

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

A cytolytic human T lymphocyte clone differentially recognizing HLA-B27 subtypes. / Miyachi, Yoshiki; Wee, Siew Lin; Chen, Li Kuang; Grumet, F. Carl; Bowman, Robert J.; Taurog, Joel D.

In: Human Immunology, Vol. 10, No. 4, 1984, p. 237-249.

Research output: Contribution to journalArticle

Miyachi, Yoshiki ; Wee, Siew Lin ; Chen, Li Kuang ; Grumet, F. Carl ; Bowman, Robert J. ; Taurog, Joel D. / A cytolytic human T lymphocyte clone differentially recognizing HLA-B27 subtypes. In: Human Immunology. 1984 ; Vol. 10, No. 4. pp. 237-249.
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abstract = "A cytolytic human T cell (CTL) clone, designated F/M-F159, has been produced, the lytic specificity of which distinguishes subtypes of HLA-B27. This was demonstrated in cell-mediated lympholysis (CML) assays of: 1) a panel of target cells from unrelated donors, 75 B27 + and 36 B27-; 2) six families, including 20 B27+ and 14 B27- individuals; and 3) B27+ and B27- variants of a B27+ lymphoblastoid cell line (LCL). Specificity of F/M-F159 for HLA-B27 was confirmed by blocking studies with monoclonal antibodies. Lysis of B27+ targets reactive with the anti-B27 monoclonal antibody B27M2 was 30-104{\%}, while lysis of B27+,B27M2- targets was 4-22{\%}. Lysis of B27- targets expressing HLA-Bw47, known to be cross-reactive with the B27M2 antibody, was 10 to 19{\%}, while lysis of all other B27 - targets was ≤10{\%}. Clone F/M-F159 lysed B27+ targets, and failed to lyse B27-targets, irrespective of the clinical status of the cell donors. It is concluded that F/M-F159 recognizes an epitope present on the majority of serologically identified HLA-B27 molecules and that this epitope is close related to, but not identical with, the epitope recognized by the antibody B27M2. These findings are interpreted as supporting a direct role for HLA-B27 in disease pathogenesis.",
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N2 - A cytolytic human T cell (CTL) clone, designated F/M-F159, has been produced, the lytic specificity of which distinguishes subtypes of HLA-B27. This was demonstrated in cell-mediated lympholysis (CML) assays of: 1) a panel of target cells from unrelated donors, 75 B27 + and 36 B27-; 2) six families, including 20 B27+ and 14 B27- individuals; and 3) B27+ and B27- variants of a B27+ lymphoblastoid cell line (LCL). Specificity of F/M-F159 for HLA-B27 was confirmed by blocking studies with monoclonal antibodies. Lysis of B27+ targets reactive with the anti-B27 monoclonal antibody B27M2 was 30-104%, while lysis of B27+,B27M2- targets was 4-22%. Lysis of B27- targets expressing HLA-Bw47, known to be cross-reactive with the B27M2 antibody, was 10 to 19%, while lysis of all other B27 - targets was ≤10%. Clone F/M-F159 lysed B27+ targets, and failed to lyse B27-targets, irrespective of the clinical status of the cell donors. It is concluded that F/M-F159 recognizes an epitope present on the majority of serologically identified HLA-B27 molecules and that this epitope is close related to, but not identical with, the epitope recognized by the antibody B27M2. These findings are interpreted as supporting a direct role for HLA-B27 in disease pathogenesis.

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