A detergent-free method for purifying caveolae membrane from tissue culture cells

Eric J. Smart, Yun Shu Ying, Chieko Mineo, Richard G W Anderson

Research output: Contribution to journalArticle

658 Scopus citations

Abstract

Current methods for purifying caveolae from tissue culture cells take advantage of the Triton X-100 insolubility of this membrane domain. To circumvent the use of detergents, we have developed a method that depends upon the unique buoyant density of caveolae membrane. The caveolae fractions that we obtain are highly enriched in caveolin. As a consequence we are able to identify caveolae-associated proteins that had previously gone undetected. Moreover, resident caveolae proteins that are soluble in Triton X-100 are retained during the isolation.

Original languageEnglish (US)
Pages (from-to)10104-10108
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number22
DOIs
StatePublished - Oct 24 1995

Keywords

  • G proteins
  • epidermal growth factor receptor
  • folate receptor

ASJC Scopus subject areas

  • General

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