Abstract
Current methods for purifying caveolae from tissue culture cells take advantage of the Triton X-100 insolubility of this membrane domain. To circumvent the use of detergents, we have developed a method that depends upon the unique buoyant density of caveolae membrane. The caveolae fractions that we obtain are highly enriched in caveolin. As a consequence we are able to identify caveolae-associated proteins that had previously gone undetected. Moreover, resident caveolae proteins that are soluble in Triton X-100 are retained during the isolation.
Original language | English (US) |
---|---|
Pages (from-to) | 10104-10108 |
Number of pages | 5 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 92 |
Issue number | 22 |
DOIs | |
State | Published - Oct 24 1995 |
Keywords
- G proteins
- epidermal growth factor receptor
- folate receptor
ASJC Scopus subject areas
- General