A diet-induced animal model of non-alcoholic fatty liver disease and hepatocellular cancer

Amon Asgharpour, Sophie C. Cazanave, Tommy Pacana, Mulugeta Seneshaw, Robert Vincent, Bubu A. Banini, Divya Prasanna Kumar, Kalyani Daita, Hae Ki Min, Faridoddin Mirshahi, Pierre Bedossa, Xiaochen Sun, Yujin Hoshida, Srinivas V. Koduru, Daniel Contaifer, Urszula Osinska Warncke, Dayanjan S. Wijesinghe, Arun J. Sanyal

Research output: Contribution to journalArticle

132 Scopus citations

Abstract

Background & Aims The lack of a preclinical model of progressive non-alcoholic steatohepatitis (NASH) that recapitulates human disease is a barrier to therapeutic development. Methods A stable isogenic cross between C57BL/6J (B6) and 129S1/SvImJ (S129) mice were fed a high fat diet with ad libitum consumption of glucose and fructose in physiologically relevant concentrations and compared to mice fed a chow diet and also to both parent strains. Results Following initiation of the obesogenic diet, B6/129 mice developed obesity, insulin resistance, hypertriglyceridemia and increased LDL-cholesterol. They sequentially also developed steatosis (4–8 weeks), steatohepatitis (16–24 weeks), progressive fibrosis (16 weeks onwards) and spontaneous hepatocellular cancer (HCC). There was a strong concordance between the pattern of pathway activation at a transcriptomic level between humans and mice with similar histological phenotypes (FDR 0.02 for early and 0.08 for late time points). Lipogenic, inflammatory and apoptotic signaling pathways activated in human NASH were also activated in these mice. The HCC gene signature resembled the S1 and S2 human subclasses of HCC (FDR 0.01 for both). Only the B6/129 mouse but not the parent strains recapitulated all of these aspects of human NAFLD. Conclusions We here describe a diet-induced animal model of non-alcoholic fatty liver disease (DIAMOND) that recapitulates the key physiological, metabolic, histologic, transcriptomic and cell-signaling changes seen in humans with progressive NASH. Lay summary We have developed a diet-induced mouse model of non-alcoholic steatohepatitis (NASH) and hepatic cancers in a cross between two mouse strains (129S1/SvImJ and C57Bl/6J). This model mimics all the physiological, metabolic, histological, transcriptomic gene signature and clinical endpoints of human NASH and can facilitate preclinical development of therapeutic targets for NASH.

Original languageEnglish (US)
Pages (from-to)579-588
Number of pages10
JournalJournal of Hepatology
Volume65
Issue number3
DOIs
StatePublished - Sep 1 2016
Externally publishedYes

Keywords

  • Drug therapy
  • Fibrosis
  • Hepatocellular carcinoma
  • Hepatocyte ballooning
  • Steatosis

ASJC Scopus subject areas

  • Hepatology

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    Asgharpour, A., Cazanave, S. C., Pacana, T., Seneshaw, M., Vincent, R., Banini, B. A., Kumar, D. P., Daita, K., Min, H. K., Mirshahi, F., Bedossa, P., Sun, X., Hoshida, Y., Koduru, S. V., Contaifer, D., Warncke, U. O., Wijesinghe, D. S., & Sanyal, A. J. (2016). A diet-induced animal model of non-alcoholic fatty liver disease and hepatocellular cancer. Journal of Hepatology, 65(3), 579-588. https://doi.org/10.1016/j.jhep.2016.05.005