A direct radioimmunoassay of corticosterone in rat serum

C. Gomez Sanchez, B. A. Murry, D. C. Kem, Norman M Kaplan

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

A direct radioimmunoassay of corticosterone is described requiring as little as 1-2 μl of rat serum. After methanol inactivation of CBG, serum was assayed with a relatively specific antibody raised against corticosterone 21 hemisuccinate. While this antibody has a significant cross reaction with cortisol, prior purification is not necessary since cortisol is not produced in the rat.

Original languageEnglish (US)
Pages (from-to)796-798
Number of pages3
JournalEndocrinology
Volume96
Issue number3
StatePublished - 1975

Fingerprint

Corticosterone
Radioimmunoassay
Hydrocortisone
Calcium Gluconate
Antibodies
Cross Reactions
Serum
Methanol
corticosterone-21-hemisuccinate

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Gomez Sanchez, C., Murry, B. A., Kem, D. C., & Kaplan, N. M. (1975). A direct radioimmunoassay of corticosterone in rat serum. Endocrinology, 96(3), 796-798.

A direct radioimmunoassay of corticosterone in rat serum. / Gomez Sanchez, C.; Murry, B. A.; Kem, D. C.; Kaplan, Norman M.

In: Endocrinology, Vol. 96, No. 3, 1975, p. 796-798.

Research output: Contribution to journalArticle

Gomez Sanchez, C, Murry, BA, Kem, DC & Kaplan, NM 1975, 'A direct radioimmunoassay of corticosterone in rat serum', Endocrinology, vol. 96, no. 3, pp. 796-798.
Gomez Sanchez C, Murry BA, Kem DC, Kaplan NM. A direct radioimmunoassay of corticosterone in rat serum. Endocrinology. 1975;96(3):796-798.
Gomez Sanchez, C. ; Murry, B. A. ; Kem, D. C. ; Kaplan, Norman M. / A direct radioimmunoassay of corticosterone in rat serum. In: Endocrinology. 1975 ; Vol. 96, No. 3. pp. 796-798.
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