A distant, cis-acting enhancer drives induction of Arf by Tgfβ in the developing eye

Yanbin Zheng, Caitlin Devitt, Jing Liu, Jie Mei, Stephen X. Skapek

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The Arf tumor suppressor represents one of several genes encoded at the Cdkn2a and Cdkn2b loci in the mouse. Beyond its role blunting the growth of incipient cancer cells, the Arf gene also plays an essential role in development: its gene product, p19Arf, is induced by Tgfβ2 in the developing eye to dampen proliferative signals from Pdgfr?, which effect ultimately fosters the vascular remodeling required for normal vision in the mouse. Mechanisms underlying Arf induction by Tgfβ2 are not fully understood. Using the chr4Δ70kb/Δ70kb mouse, we now show that deletion of the coronary artery disease (CAD) risk interval lying upstream of the Cdkn2a/b locus represses developmentally-timed induction of Arf resulting in eye disease mimicking the persistent hyperplastic primary vitreous (PHPV) found in Arf-null mice and in children. Using mouse embryo fibroblasts, we demonstrate that Arf induction by Tgfβ is blocked in cis to the 70kb deletion, but Arf induction by activated RAS and cell culture "shock" is not. Finally, we show that Arf induction by Tgfβ is derailed by preventing RNA polymerase II recruitment following Smad 2/3 binding to the promoter. These findings provide the first evidence that the CAD risk interval, located at a distance from Arf, acts as a cis enhancer of Tgfβ2-driven induction of Arf during development.

Original languageEnglish (US)
Pages (from-to)49-57
Number of pages9
JournalDevelopmental Biology
Volume380
Issue number1
DOIs
StatePublished - Aug 1 2013

Fingerprint

Coronary Artery Disease
Persistent Hyperplastic Primary Vitreous
Genes
Eye Diseases
RNA Polymerase II
Shock
Neoplasms
Embryonic Structures
Cell Culture Techniques
Fibroblasts
Drive
Growth
Vascular Remodeling

Keywords

  • 9p21
  • Arf
  • PHPV
  • Tgfβ

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

A distant, cis-acting enhancer drives induction of Arf by Tgfβ in the developing eye. / Zheng, Yanbin; Devitt, Caitlin; Liu, Jing; Mei, Jie; Skapek, Stephen X.

In: Developmental Biology, Vol. 380, No. 1, 01.08.2013, p. 49-57.

Research output: Contribution to journalArticle

@article{6b8a8fd9f6284525b5946ded49477fbc,
title = "A distant, cis-acting enhancer drives induction of Arf by Tgfβ in the developing eye",
abstract = "The Arf tumor suppressor represents one of several genes encoded at the Cdkn2a and Cdkn2b loci in the mouse. Beyond its role blunting the growth of incipient cancer cells, the Arf gene also plays an essential role in development: its gene product, p19Arf, is induced by Tgfβ2 in the developing eye to dampen proliferative signals from Pdgfr?, which effect ultimately fosters the vascular remodeling required for normal vision in the mouse. Mechanisms underlying Arf induction by Tgfβ2 are not fully understood. Using the chr4Δ70kb/Δ70kb mouse, we now show that deletion of the coronary artery disease (CAD) risk interval lying upstream of the Cdkn2a/b locus represses developmentally-timed induction of Arf resulting in eye disease mimicking the persistent hyperplastic primary vitreous (PHPV) found in Arf-null mice and in children. Using mouse embryo fibroblasts, we demonstrate that Arf induction by Tgfβ is blocked in cis to the 70kb deletion, but Arf induction by activated RAS and cell culture {"}shock{"} is not. Finally, we show that Arf induction by Tgfβ is derailed by preventing RNA polymerase II recruitment following Smad 2/3 binding to the promoter. These findings provide the first evidence that the CAD risk interval, located at a distance from Arf, acts as a cis enhancer of Tgfβ2-driven induction of Arf during development.",
keywords = "9p21, Arf, PHPV, Tgfβ",
author = "Yanbin Zheng and Caitlin Devitt and Jing Liu and Jie Mei and Skapek, {Stephen X.}",
year = "2013",
month = "8",
day = "1",
doi = "10.1016/j.ydbio.2013.05.003",
language = "English (US)",
volume = "380",
pages = "49--57",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - A distant, cis-acting enhancer drives induction of Arf by Tgfβ in the developing eye

AU - Zheng, Yanbin

AU - Devitt, Caitlin

AU - Liu, Jing

AU - Mei, Jie

AU - Skapek, Stephen X.

PY - 2013/8/1

Y1 - 2013/8/1

N2 - The Arf tumor suppressor represents one of several genes encoded at the Cdkn2a and Cdkn2b loci in the mouse. Beyond its role blunting the growth of incipient cancer cells, the Arf gene also plays an essential role in development: its gene product, p19Arf, is induced by Tgfβ2 in the developing eye to dampen proliferative signals from Pdgfr?, which effect ultimately fosters the vascular remodeling required for normal vision in the mouse. Mechanisms underlying Arf induction by Tgfβ2 are not fully understood. Using the chr4Δ70kb/Δ70kb mouse, we now show that deletion of the coronary artery disease (CAD) risk interval lying upstream of the Cdkn2a/b locus represses developmentally-timed induction of Arf resulting in eye disease mimicking the persistent hyperplastic primary vitreous (PHPV) found in Arf-null mice and in children. Using mouse embryo fibroblasts, we demonstrate that Arf induction by Tgfβ is blocked in cis to the 70kb deletion, but Arf induction by activated RAS and cell culture "shock" is not. Finally, we show that Arf induction by Tgfβ is derailed by preventing RNA polymerase II recruitment following Smad 2/3 binding to the promoter. These findings provide the first evidence that the CAD risk interval, located at a distance from Arf, acts as a cis enhancer of Tgfβ2-driven induction of Arf during development.

AB - The Arf tumor suppressor represents one of several genes encoded at the Cdkn2a and Cdkn2b loci in the mouse. Beyond its role blunting the growth of incipient cancer cells, the Arf gene also plays an essential role in development: its gene product, p19Arf, is induced by Tgfβ2 in the developing eye to dampen proliferative signals from Pdgfr?, which effect ultimately fosters the vascular remodeling required for normal vision in the mouse. Mechanisms underlying Arf induction by Tgfβ2 are not fully understood. Using the chr4Δ70kb/Δ70kb mouse, we now show that deletion of the coronary artery disease (CAD) risk interval lying upstream of the Cdkn2a/b locus represses developmentally-timed induction of Arf resulting in eye disease mimicking the persistent hyperplastic primary vitreous (PHPV) found in Arf-null mice and in children. Using mouse embryo fibroblasts, we demonstrate that Arf induction by Tgfβ is blocked in cis to the 70kb deletion, but Arf induction by activated RAS and cell culture "shock" is not. Finally, we show that Arf induction by Tgfβ is derailed by preventing RNA polymerase II recruitment following Smad 2/3 binding to the promoter. These findings provide the first evidence that the CAD risk interval, located at a distance from Arf, acts as a cis enhancer of Tgfβ2-driven induction of Arf during development.

KW - 9p21

KW - Arf

KW - PHPV

KW - Tgfβ

UR - http://www.scopus.com/inward/record.url?scp=84881526483&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881526483&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2013.05.003

DO - 10.1016/j.ydbio.2013.05.003

M3 - Article

VL - 380

SP - 49

EP - 57

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -